| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,360,083 | T→C | 27.7% | F344S (TTC→TCC) | gltD → | glutamate synthase, 4Fe‑4S protein, small subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,360,083 | 0 | T | C | 27.7% | 69.9 / 32.9 | 47 | F344S (TTC→TCC) | gltD | glutamate synthase, 4Fe‑4S protein, small subunit |
| Reads supporting (aligned to +/- strand): ref base T (20/14); new base C (6/7); total (26/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.20e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01 | |||||||||||
ATGAAGAGAACATGCCGGGTTCCCGCCGCGAAGTGAAAAACGCGCGGGAAGAAGGCGTAGAGTTCAAATTCAACGTCCAGCCGCTGGGTATTGAAGTGAACGGTAACGGCAAAGTCAGCGGCGTAAAAAT > W3110S.gb/3360014‑3360143 | atgaAGAGAACATGCCGGGTTCCCGCCGCGAAGTGAAAAACGCGCGGGAAGAAGGCGTAGAGTTCAAATTc < 1:686703/71‑1 (MQ=255) tgaAGAGAACATGCCGGGTTCCCGCCGCGAAGTGAAAAACGCGCGGGAAGAAGGCGTAGAGTTCAAATTc < 1:453384/70‑1 (MQ=255) gagaACATGCCGGGTTCCCGCCGCGAAGTGAAAAACGCGCGGGAAGAAGGCGTAGAGTTCAAATTCAACGt < 1:835677/71‑1 (MQ=255) gaACATGCCGGGTTCCCGCCGCGAAGTGAAAAACGCGCGGGAAGAAGGCGTAGAGTTCAAATTCAACGTc < 1:3852132/70‑1 (MQ=255) gggTTCCCGCCGCGAAGTGAAAAACGCGCGGGAAGAAGGCGTAGAGTTCAAATTCAACGTCCAGCCGCTg > 1:3098244/1‑70 (MQ=255) ggTTCCCGCCGCGAAGTGAAAAACGCGCGGGAAGAAGGCGTAGAGTTCAAATTCAACGTCCAGCCGCTggg < 1:1252437/71‑1 (MQ=255) ttCCCGCCGCGAAGTGAAAAACGCGCGGGAAGAAGGCGTAGAGTTCAAATCCAACGTc < 1:3002026/58‑1 (MQ=255) ccgccgCGAAGTGAAAAACGCGCGGGAAGAAGGCGTAGAGTTCAAATTc < 1:882197/49‑1 (MQ=255) ccgccgCGAAGTGAAAAACGCGCGGGAAGAAGGCGTAGAGTTCAAATTCAACGTCCAGCCGCTGGGTATTg < 1:1669437/71‑1 (MQ=255) ccgcGAAGTGAAAAACGCGCGGGAAGAAGGCGTAGAGTTCAAATTCAACGTCCATCCg > 1:339044/1‑58 (MQ=255) cgcgAAGTGAAAAACGCGCGGGAAGAAGGCGTAGAGTTCAAATTCAACGTCCAGCCGCTGGGTATTGAAGt > 1:2610306/1‑71 (MQ=255) cgcgAAGTGAAAAACGCGCGGGAAGAAGGCGTAGAGTTCAAATCCAACGTCCAGCCGCTGGGTATTGAAGt < 1:2415270/71‑1 (MQ=255) cgAAGTGAAAAACGCGCGGGAAGAAGGCGTAGAGTTCAAATCCAACGTCCAGCCGCTGGGTATTGAAGTGa < 1:2836503/71‑1 (MQ=255) gTGAAAAACGCGCGGGAAGAAGGCGTAGAGTTCAAATCCa < 1:913156/40‑1 (MQ=255) tGAAAAACGCGCGGGAAGAAGGCGTAGAGTTCAAATTCAACGTCCAGCCGCTGGGTATTGAAGTGAACGGt < 1:1424354/71‑1 (MQ=255) tGAAAAACGCGCGGGAAGAAGGCGTAGAGTTCAAATCCAACGTCCAGCCGCTGGGTATTGAAGTGAACGGt < 1:1753856/71‑1 (MQ=255) aaaaaCTCGCGGGAAGAAGGCGTAGAGTTCAAATTCAACGTCCAGCCGCTGGCTATTGAAGTGAACGGTaa > 1:6294/1‑71 (MQ=255) aaaaaCGCGCGGGAAGAAGGCGTATAGTTCAAATTCAACGTCCAGCCGCTGGGTATTGAAGTGAACGGTaa > 1:3821765/1‑71 (MQ=255) aaaaaCGCGCGGGAAGAAGGCGTAGAGTTCAAATTCAACGTCCAGCCg > 1:4506880/1‑48 (MQ=255) aaaaaCGCGCGGGAAGAAGGCGTAGAGTTCAAATTCAACGTCCAGCCGCTGGGTATTGAAGTGAACGGTaa > 1:4402941/1‑71 (MQ=255) aaaaaCGCGCGGGAAGAAGGCGTAGAGTTCAAATTCAACGTCCAGCCGCTGGGTATTGAAGTGAACGGTaa > 1:2323809/1‑71 (MQ=255) aaaCGCGCGGGAAGAAGGCGTAGAGTTCAAATTCAACGTCCAGCCGCTGGGTATTGAAGTg < 1:959737/61‑1 (MQ=255) aaCGCGCGGGAAGAAGGCGTAGAGTTCAAATTCAACGTCCAGCCGCTGGGTATTGAAGTg < 1:2475609/60‑1 (MQ=255) cgcgcgGGAAGAAGGCGTAGAGTTCAAATCCAACGTCCAGCCGCTGGGTATTGAAGTGAACGGTAACGGc > 1:1079185/1‑70 (MQ=255) cgcgcgGGAAGAAGGCGTAGAGTTCAAATCCAACGTCCAGCCGCTGGGTATTGAAGTGAACGGTAACGGCa < 1:4444843/71‑1 (MQ=255) gggAAGAAGGCGTAGAGTTCAAATTCAACGTCCAGCCGCTGGGTATTGAAGTGAACGGTAACGGCAAAGTc < 1:619876/71‑1 (MQ=255) agaagGCGTAGAGTTCAAATTCAACGTCCAGCCGCTGGGTATTGAAGTGAACGGTAACGGCa < 1:3134597/62‑1 (MQ=255) agaagGCGTAGAGTTCAAATTCAACGTCCAGCCGCTGGGTATTGAAGTGAACGGTAACGGCAAAGTCAgcg > 1:3178033/1‑71 (MQ=255) gaagGCGTAGAGTTCAAATTCAACGTCCAGCCGCTGGGTATTGAAGTGAACGGTAACGGCAAAGTCAgcgg > 1:4476900/1‑71 (MQ=255) aagGCGTAGAGTTCAAATTCAACGTCCAGCCGCTGGGTATTGAAGTGAACGGTAACGGCAAAGTCAgcggc > 1:2325415/1‑71 (MQ=255) ggCGTAGAGTTCAAATTCAACGTCCAGCCGCTGGGTATTGa < 1:2660904/41‑1 (MQ=255) ggCGTAGAGTTCAAATCCAACGTCCAGCCGCTGGGTATTGAAGTGAACGGTAACGGCAAAGTCAgcggcg > 1:3448540/1‑70 (MQ=255) ggCGTAGAGTTCAAATCCAACGTCCAGCCGCTGGGTATTGAAGTGAACGGTAACGGCAAAGTCAgcggcg > 1:192274/1‑70 (MQ=255) ggCGTAGAGTTCAAATCCAACGTCCAGCCGCTGGGTATTGAAGTGAACGGTAACGGCAAAGTCAgcggcg > 1:3591955/1‑70 (MQ=255) cGTAGAGTTCAAATTCAACGTCCAGCCGCTGGGTATTGAAGTGaa > 1:2882987/1‑45 (MQ=255) cGTAGAGTTCAAATTCAACGTCCAGCCGCTGGGTATTGAAGTGAACGGTAACGGCAAAGTCAGCGGCGTa < 1:554496/70‑1 (MQ=255) tAGAGTTCAAATCCAACGTCCAGCCGCTGGGTATTGa < 1:565514/37‑1 (MQ=255) agagTTCAAATTCAACGTCCAGCCGCTGGGTATTGAAGTGAACGGTAACGGCAAAGTCAGCGGCGTaaaaa > 1:802219/1‑71 (MQ=255) gagTTCAAATTCAACGTCCAGCCGCTGGGTATTGAAGTGAACGGTAACGGCAAAGTCAgc > 1:2449265/1‑60 (MQ=255) gagTTCAAATTCAACGTCCAGCCGCTGGGTATTGAAGTGAACGGTAACGGCAAAGTCAGCGGCGTaaaaa > 1:1954456/1‑70 (MQ=255) gagTTCAAATTCAACGTCCAGCCGCTGGGTATTGAAGTGAACGGTAACGGCAAAGTCAGCGGCGTaaaa > 1:2765519/1‑69 (MQ=255) gagTTCAAATTCAACGTCCAGCCGCTGGGTATTGAAGTGAACGGTAACGGCAAAGTCAGCGGCGTAAAAAt > 1:4477996/1‑71 (MQ=255) gagTTCAAATCCAACGTCCAGCCGCTGGGTATTGaa > 1:2993273/1‑36 (MQ=255) cAAATTCCACGTCCAGCCGCTGGGTATTGAAGTGAACGGTAACGGCAAAGTCAgcgg > 1:3622880/1‑57 (MQ=255) cAAATCCAACGTCCAGCCGCTGGGTATTGAAGTGAACGGTAACGGCAAAGTCa > 1:646268/1‑53 (MQ=255) aaaTTCAACGTCCAGCCGCTGGGTATTGAAGTGAACGGTaa > 1:2337992/1‑41 (MQ=255) aaaTTCAACGTCCAGCCGCTGGGTATTGAAGTGAACGGTaa > 1:1031198/1‑41 (MQ=255) | ATGAAGAGAACATGCCGGGTTCCCGCCGCGAAGTGAAAAACGCGCGGGAAGAAGGCGTAGAGTTCAAATTCAACGTCCAGCCGCTGGGTATTGAAGTGAACGGTAACGGCAAAGTCAGCGGCGTAAAAAT > W3110S.gb/3360014‑3360143 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |