| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,383,928 | C→T | 18.4% | A66T (GCG→ACG) | mdh ← | malate dehydrogenase, NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,383,928 | 0 | C | T | 18.4% | 86.3 / 12.4 | 38 | A66T (GCG→ACG) | mdh | malate dehydrogenase, NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base C (17/14); new base T (3/4); total (20/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
CGATCCATACCCGGTTTACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGC > W3110S.gb/3383864‑3383997 | cGATCCATACCCGGTTTACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGt < 1:2571360/71‑1 (MQ=255) ccATACCCGGTTTACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCa > 1:3595136/1‑71 (MQ=255) aTACCCGGTTTACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATc < 1:2873840/71‑1 (MQ=255) ttACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCg > 1:3659987/1‑52 (MQ=255) tACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGa > 1:657559/1‑70 (MQ=255) aCGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGaa > 1:1864695/1‑70 (MQ=255) gcgcTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGaaaaa < 1:2386136/71‑1 (MQ=255) cGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCttt > 1:3155809/1‑70 (MQ=255) tGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGAttt > 1:3223755/1‑71 (MQ=255) cAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCTTTGAtttt > 1:571146/1‑70 (MQ=255) agagATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCTTTGAtttt < 1:2184136/69‑1 (MQ=255) gagaTAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTcac < 1:2625487/71‑1 (MQ=255) aTAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCAcag > 1:2458057/1‑70 (MQ=255) tAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCTTTGAtttt < 1:3618319/64‑1 (MQ=255) aaGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCAcagcag > 1:3038636/1‑71 (MQ=255) cGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAgg > 1:3164732/1‑70 (MQ=255) gACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGa < 1:4023793/71‑1 (MQ=255) aCATCTGCGCCTTCCAGAGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGa < 1:2195539/70‑1 (MQ=255) cATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGata < 1:2741044/71‑1 (MQ=255) cATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGata < 1:4540734/71‑1 (MQ=255) tCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGAtt < 1:4294002/51‑1 (MQ=255) tCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATg > 1:2853548/1‑71 (MQ=255) cTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATgg > 1:4107376/1‑71 (MQ=255) cTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGata > 1:632401/1‑68 (MQ=255) tGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCttt > 1:3057072/1‑45 (MQ=255) tGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTca > 1:2537265/1‑53 (MQ=255) tGCGCCTTCCAGCGCCGGAGTCGCATCTTCACAAGAAAAAc > 1:335551/1‑41 (MQ=255) cgcCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCt < 1:1742397/70‑1 (MQ=255) gcCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCt > 1:3249189/1‑69 (MQ=255) cTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTc > 1:3816628/1‑47 (MQ=255) ttCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGAtttt < 1:3381170/45‑1 (MQ=255) ttCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTc > 1:1452865/1‑46 (MQ=255) tCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCt < 1:2968524/36‑1 (MQ=255) ccAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATc > 1:4583841/1‑70 (MQ=255) ccAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATc < 1:2905815/70‑1 (MQ=255) ccAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATc < 1:2156887/70‑1 (MQ=255) ccAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATc < 1:1785616/70‑1 (MQ=255) cAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCg < 1:1511369/70‑1 (MQ=255) gcgcCGGAGTCGCATCTTCACCAGAAAAACCTTTGAtttt < 1:3864037/40‑1 (MQ=255) gcgcCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACa < 1:3213627/71‑1 (MQ=255) gcgcCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTAACAGCAGTAggg < 1:763540/53‑1 (MQ=255) cgcCGGAGTCGCATCTTCACCAGAAAAACCTTTGAtt > 1:1566643/1‑37 (MQ=255) gcCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGc < 1:2795204/71‑1 (MQ=255) ccGGAGTCGCATCTTCACCAGAAAAACCTTTGAtttt < 1:3707469/37‑1 (MQ=255) | CGATCCATACCCGGTTTACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGC > W3110S.gb/3383864‑3383997 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |