Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 3,383,928 C→T 18.4% A66T (GCG→ACG)  mdh ← malate dehydrogenase, NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,383,9280CT18.4% 86.3 / 12.4 38A66T (GCG→ACG) mdhmalate dehydrogenase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base C (17/14);  new base T (3/4);  total (20/18)
Fisher's exact test for biased strand distribution p-value = 6.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

CGATCCATACCCGGTTTACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGC  >  W3110S.gb/3383864‑3383997
                                                                |                                                                     
cGATCCATACCCGGTTTACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGt                                                                 <  1:2571360/71‑1 (MQ=255)
    ccATACCCGGTTTACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCa                                                             >  1:3595136/1‑71 (MQ=255)
      aTACCCGGTTTACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATc                                                           <  1:2873840/71‑1 (MQ=255)
               ttACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCg                                                                     >  1:3659987/1‑52 (MQ=255)
                tACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGa                                                  >  1:657559/1‑70 (MQ=255)
                 aCGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGaa                                                 >  1:1864695/1‑70 (MQ=255)
                   gcgcTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGaaaaa                                              <  1:2386136/71‑1 (MQ=255)
                         cGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCttt                                         >  1:3155809/1‑70 (MQ=255)
                             tGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGAttt                                    >  1:3223755/1‑71 (MQ=255)
                               cAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCTTTGAtttt                                   >  1:571146/1‑70 (MQ=255)
                                agagATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCTTTGAtttt                                   <  1:2184136/69‑1 (MQ=255)
                                 gagaTAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTcac                                <  1:2625487/71‑1 (MQ=255)
                                    aTAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCAcag                              >  1:2458057/1‑70 (MQ=255)
                                     tAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCTTTGAtttt                                   <  1:3618319/64‑1 (MQ=255)
                                      aaGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCAcagcag                           >  1:3038636/1‑71 (MQ=255)
                                           cGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAgg                       >  1:3164732/1‑70 (MQ=255)
                                            gACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGa                     <  1:4023793/71‑1 (MQ=255)
                                             aCATCTGCGCCTTCCAGAGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGa                     <  1:2195539/70‑1 (MQ=255)
                                              cATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGata                   <  1:2741044/71‑1 (MQ=255)
                                              cATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGata                   <  1:4540734/71‑1 (MQ=255)
                                                tCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGAtt                                     <  1:4294002/51‑1 (MQ=255)
                                                tCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATg                 >  1:2853548/1‑71 (MQ=255)
                                                 cTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATgg                >  1:4107376/1‑71 (MQ=255)
                                                 cTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGata                   >  1:632401/1‑68 (MQ=255)
                                                  tGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCttt                                         >  1:3057072/1‑45 (MQ=255)
                                                  tGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTca                                 >  1:2537265/1‑53 (MQ=255)
                                                  tGCGCCTTCCAGCGCCGGAGTCGCATCTTCACAAGAAAAAc                                             >  1:335551/1‑41 (MQ=255)
                                                    cgcCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCt              <  1:1742397/70‑1 (MQ=255)
                                                     gcCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCt              >  1:3249189/1‑69 (MQ=255)
                                                       cTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTc                                  >  1:3816628/1‑47 (MQ=255)
                                                        ttCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGAtttt                                   <  1:3381170/45‑1 (MQ=255)
                                                        ttCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTc                                  >  1:1452865/1‑46 (MQ=255)
                                                         tCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCt                                           <  1:2968524/36‑1 (MQ=255)
                                                          ccAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATc        >  1:4583841/1‑70 (MQ=255)
                                                          ccAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATc        <  1:2905815/70‑1 (MQ=255)
                                                          ccAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATc        <  1:2156887/70‑1 (MQ=255)
                                                          ccAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATc        <  1:1785616/70‑1 (MQ=255)
                                                           cAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCg       <  1:1511369/70‑1 (MQ=255)
                                                             gcgcCGGAGTCGCATCTTCACCAGAAAAACCTTTGAtttt                                   <  1:3864037/40‑1 (MQ=255)
                                                             gcgcCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACa    <  1:3213627/71‑1 (MQ=255)
                                                             gcgcCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTAACAGCAGTAggg                      <  1:763540/53‑1 (MQ=255)
                                                              cgcCGGAGTCGCATCTTCACCAGAAAAACCTTTGAtt                                     >  1:1566643/1‑37 (MQ=255)
                                                               gcCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGc  <  1:2795204/71‑1 (MQ=255)
                                                                ccGGAGTCGCATCTTCACCAGAAAAACCTTTGAtttt                                   <  1:3707469/37‑1 (MQ=255)
                                                                |                                                                     
CGATCCATACCCGGTTTACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGC  >  W3110S.gb/3383864‑3383997

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: