| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,404,829 | C→G | 18.4% | intergenic (+516/‑462) | yhdH → / → accB | predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding/acetyl CoA carboxylase, BCCP subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,404,829 | 0 | C | G | 18.4% | 125.6 / 22.1 | 60 | intergenic (+516/‑462) | yhdH/accB | predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding/acetyl CoA carboxylase, BCCP subunit |
| Reads supporting (aligned to +/- strand): ref base C (25/23); new base G (4/7); total (29/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.06e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
TTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCGATGATGGCGCTAATTT > W3110S.gb/3404762‑3404898 | ttGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATGTTc > 1:2500991/1‑71 (MQ=255) gTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCg < 1:3993300/70‑1 (MQ=255) tCGCGATCCTTGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCa < 1:2532224/71‑1 (MQ=255) tCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCt < 1:3134286/66‑1 (MQ=255) gcgATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAg < 1:1225222/70‑1 (MQ=255) ccTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATGTTCGCAGggctg > 1:1755356/1‑70 (MQ=255) cTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGggctggc > 1:232711/1‑71 (MQ=255) cTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGggctggc > 1:2284170/1‑71 (MQ=255) ggCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgctt > 1:1057619/1‑71 (MQ=255) ggCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgctt > 1:2660788/1‑71 (MQ=255) gCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgctt < 1:456655/70‑1 (MQ=255) tCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCg < 1:2832553/55‑1 (MQ=255) ccTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGggctgg > 1:1527993/1‑62 (MQ=255) ccTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGggct > 1:1410422/1‑60 (MQ=255) ccTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttcagc > 1:1055616/1‑71 (MQ=255) ccTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttcagc > 1:3903040/1‑71 (MQ=255) tACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTc < 1:1413741/50‑1 (MQ=255) cATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCa > 1:1746200/1‑51 (MQ=255) cATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttcagctttt > 1:3605287/1‑71 (MQ=255) cATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttcagctttt > 1:2532355/1‑71 (MQ=255) attattTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttcagcttttc < 1:314889/71‑1 (MQ=255) ttattTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATGTTCGCAGTGCTGgcttttcagcttttca < 1:621613/71‑1 (MQ=255) ttattTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATGTTCGCAGGGCTGgcttttcagcttttca < 1:270712/71‑1 (MQ=255) ttattTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttc < 1:4359250/62‑1 (MQ=255) tattTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATGTTCGCAGGGCTGGCTTTTCAGCTTTTCAc > 1:3324647/1‑71 (MQ=255) tattTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATGTTCGCAGGGCTGGCTTTTCAGCTTTTCAc > 1:856261/1‑71 (MQ=255) tattTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCAc > 1:2161732/1‑71 (MQ=255) attTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTc > 1:99272/1‑44 (MQ=255) attTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttcag > 1:1587896/1‑62 (MQ=255) tttGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATGTTCGCAGGGCTGGCTTTTCAGCTTTTCACCt < 1:430309/71‑1 (MQ=255) tttGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATGTTCGCAGGGCTGGCTTTTCAGCTTTTCACCt < 1:3765975/71‑1 (MQ=255) tgtgGTCTGTGAAGATTATCTCATTGCAGCCCCTCATGTTCGCAGGGCTGGCTTTTCAGCTTTTCACCtt < 1:1726970/70‑1 (MQ=255) ggTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGc < 1:242233/40‑1 (MQ=255) ggTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGc < 1:3372139/40‑1 (MQ=255) tCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTta < 1:1531834/71‑1 (MQ=255) tgtgAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTta > 1:3595052/1‑69 (MQ=255) gAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTGACCTTACGTTATaag > 1:432656/1‑70 (MQ=255) gAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATaag > 1:2199307/1‑70 (MQ=255) aaGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATaaga < 1:2936107/70‑1 (MQ=255) aaGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATaaga < 1:1722490/70‑1 (MQ=255) aGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATaagaag < 1:1306317/71‑1 (MQ=255) gATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGt < 1:524240/71‑1 (MQ=255) aTTATCTCATTGCAGCCCCTCATGTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTtat < 1:2252226/63‑1 (MQ=255) aTTATCTCATTGCAGCCCCTCATATTCGCAGGGCTGGCTTTTCAGCTTTTCAACTTACGTTATAAGAAGtt < 1:2381939/71‑1 (MQ=38) tATCTCATTGCAGCCCCTCATCTTCGCAGggctgg > 1:1046318/1‑35 (MQ=255) tATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTcc > 1:3581945/1‑71 (MQ=255) aTTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTc < 1:2725095/68‑1 (MQ=255) aTTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTc < 1:1909635/68‑1 (MQ=255) aTTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTc < 1:4597275/68‑1 (MQ=255) aTTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTc < 1:1668229/68‑1 (MQ=255) aTTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTc < 1:3576632/68‑1 (MQ=255) ccccTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATaaga > 1:4203393/1‑52 (MQ=255) cTCATGTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACg < 1:2904677/41‑1 (MQ=255) tCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCgatga > 1:4391525/1‑62 (MQ=255) tCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCgatg > 1:4019015/1‑61 (MQ=255) cATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATaag < 1:2769524/46‑1 (MQ=255) cATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCga > 1:4545412/1‑58 (MQ=255) cATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCGATGATGGCGCTAAt > 1:3455215/1‑71 (MQ=255) tCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATaaga < 1:3462048/45‑1 (MQ=255) tCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCGATGATGGCGCTAAttt > 1:3242236/1‑71 (MQ=255) | TTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCGATGATGGCGCTAATTT > W3110S.gb/3404762‑3404898 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |