| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,488,502 | C→T | 23.6% | L47L (CTG→TTG) | yijO → | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,488,502 | 0 | C | T | 23.6% | 147.8 / 43.8 | 77 | L47L (CTG→TTG) | yijO | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (27/31); new base T (8/10); total (36/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGCAGTAACGGCCCGGTGCCCGATCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGTTAGTTCCCGGCGATGTGCTGTACG > W3110S.gb/3488439‑3488565 | agcagTAACGGCCCCGTGCCCGATCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAgg < 1:4085427/71‑1 (MQ=255) gTAACGGCCCGGTGCCCGATCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGTTGGAAGGTGAg < 1:1755372/71‑1 (MQ=255) tAACGGCCCGGTGCCCGATCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTgg < 1:3400544/62‑1 (MQ=255) tAACGGCCCGGTGCCCGATCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGa < 1:992353/63‑1 (MQ=255) aCGGCCCGGTGCCCGATCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGTTgg > 1:1876105/1‑60 (MQ=255) aCGGCCCGGTGCCCGATCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGTTGGAAGGTGAGttt > 1:3291391/1‑71 (MQ=255) cGGCCCGGTGCCCGATCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTttg > 1:842966/1‑71 (MQ=255) gCCCGGTGCCCGATCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTttgtt < 1:4579294/71‑1 (MQ=255) ggTGCCCGATCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATa > 1:176876/1‑71 (MQ=255) gTGCCCGATCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGTTGGAAGGTGAGTTTGTTGATAc < 1:3993289/71‑1 (MQ=255) cccGATCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTttg < 1:3218716/61‑1 (MQ=255) cccGATCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTttg < 1:2087816/61‑1 (MQ=255) cccGATCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTttg < 1:3899245/61‑1 (MQ=255) cGATCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGgcg > 1:4487190/1‑71 (MQ=255) tCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTg > 1:765432/1‑71 (MQ=255) cTTGGGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTttg < 1:1220314/55‑1 (MQ=255) cTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGTTGGAAGGTGAGTTTGTTGa > 1:1845469/1‑59 (MQ=255) tGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGc < 1:51496/38‑1 (MQ=255) gCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAg < 1:1200825/48‑1 (MQ=255) gCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAg < 1:3347681/48‑1 (MQ=255) gCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAg > 1:374236/1‑70 (MQ=255) gTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGtt > 1:928213/1‑48 (MQ=255) gTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCaa > 1:1656087/1‑71 (MQ=255) gTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCaa < 1:2579202/71‑1 (MQ=255) tATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCaa < 1:3924748/70‑1 (MQ=255) aTCAGGTCGATTTTCCACGGCTGGAAATTGTGTTGGAAGGTGAGTTTGTTGa > 1:4106877/1‑52 (MQ=255) aTCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACg > 1:3925123/1‑71 (MQ=255) aTCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACg > 1:3097174/1‑71 (MQ=255) aTCAGGTCGATTTTCCACGGCTGGAAATTGTGATGGAAGGTGAGttt > 1:4398511/1‑47 (MQ=255) tCAGGTCGATTTTCCACGGCTGGAAATTGTGTTGGAAGGTGAGTTTGTTGATACCGGCGCTgg < 1:4520251/63‑1 (MQ=255) tCAGGTCGATTTTCCACGGCTGGAAATTGTGTTGGAAGGTCAGTTTGTTGAt < 1:2975201/52‑1 (MQ=255) tCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTttgtt < 1:4041060/49‑1 (MQ=255) tCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGa > 1:348082/1‑51 (MQ=255) tCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGa > 1:2806629/1‑51 (MQ=255) tCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCa < 1:4399134/67‑1 (MQ=255) tCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGt > 1:4053459/1‑71 (MQ=255) aGGTCGATTTTCCATGGCTGGAAATTGTGTTGGAAGGTGAGttt > 1:1550314/1‑44 (MQ=38) aGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGttt > 1:3273100/1‑44 (MQ=255) aGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGtt < 1:342289/70‑1 (MQ=255) aGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGtt < 1:4417951/70‑1 (MQ=255) ggTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGTTAg < 1:2093745/71‑1 (MQ=255) gTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGa < 1:1077988/47‑1 (MQ=255) tCGATTTTCCACGGCTGGAAATTGTGTTGGAAGGTTAGTTTGTTGATACCGGCGCTGGAGCAACGtt < 1:2526624/67‑1 (MQ=255) cGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGTTAGTTc > 1:1358373/1‑71 (MQ=255) cGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGTTAGTTc > 1:3490306/1‑71 (MQ=255) cGATTTTACACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGTTAGTTc > 1:959200/1‑71 (MQ=255) gATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGtt < 1:2201089/38‑1 (MQ=255) aTTTTCCACGGCTGGAAATTGTGTTGGAAGGTGAGTttg < 1:1625331/39‑1 (MQ=255) ttttCCACGGCTGGAAATTGTGCTGGAAGGTGAGTttg < 1:1467182/38‑1 (MQ=255) ttttCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGa < 1:2586142/42‑1 (MQ=255) tttCCACGGCTGGAAATTGTGTTGGAAGGTGAGTttgtt > 1:1260547/1‑39 (MQ=255) tttCCACGGCTGGAAATTGTGCTGGAAGGTGAGtt < 1:1873084/35‑1 (MQ=255) tttCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGTTAGtt > 1:4616924/1‑66 (MQ=255) tttCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGTTAGtt > 1:4611652/1‑66 (MQ=255) tttCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGTTAGtt > 1:287365/1‑66 (MQ=255) tttCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGTTAGTTCCCgg > 1:4515304/1‑71 (MQ=255) ttCCACGGCTGGAAATTGTGCTGGAAGGTGAGTttgtt > 1:4421720/1‑38 (MQ=255) ttCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGTTAGTTCCCGGc > 1:1310555/1‑71 (MQ=255) ttCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGTTAGTTCCCGGc > 1:3309978/1‑71 (MQ=255) tCCACGGCTGGAAATTGTGTTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGTTAGTTCCCGGc < 1:2072000/70‑1 (MQ=255) tCCACGGCTGGAAATTGTGCTGGAAGGTGAGTttgtt > 1:1640635/1‑37 (MQ=255) ggCTGGAAATTGTGTTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGTTAGTTCCCGGCGAtgtg > 1:2178958/1‑71 (MQ=255) ggCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGTTAGTTCCCGGCGAtgtg < 1:1012866/71‑1 (MQ=255) gCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGTTAGTTCCCGGCGATGTGc > 1:3808564/1‑71 (MQ=255) tGGAAATTGTGTTGGAAGGTGAGTTTGTTGATACCGGCGCTGGa < 1:2635934/44‑1 (MQ=255) gAAATTGTGTTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGt < 1:3044969/49‑1 (MQ=255) gAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCaa > 1:474098/1‑46 (MQ=255) gAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGtt < 1:49215/50‑1 (MQ=255) gAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGTTAGTTCCCGGCGa < 1:2771454/62‑1 (MQ=255) gAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGTTAGTTCCCGGCGATGTGCTg < 1:4462366/69‑1 (MQ=255) aaaTTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAAc < 1:2076061/46‑1 (MQ=255) aaaTTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGtt < 1:2574192/49‑1 (MQ=255) aaTTGTGTTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGTTa > 1:4635338/1‑49 (MQ=255) aaTTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGtt < 1:1337716/48‑1 (MQ=255) aaTTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGTTAGTTCCCGGCGATGTGCTGTACg > 1:1162586/1‑71 (MQ=255) aTTGTGTTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGtt < 1:2842063/47‑1 (MQ=255) aTTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGtt < 1:2086435/47‑1 (MQ=255) ttGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGt < 1:2307745/45‑1 (MQ=255) cTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGTTAGTTCCCGGCGAtgtg < 1:1880247/57‑1 (MQ=255) | AGCAGTAACGGCCCGGTGCCCGATCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGTTAGTTCCCGGCGATGTGCTGTACG > W3110S.gb/3488439‑3488565 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |