| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,515,054 | A→G | 9.1% | N41D (AAC→GAC) | hslU → | molecular chaperone and ATPase component of HslUV protease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,515,054 | 0 | A | G | 9.1% | 113.8 / 4.2 | 44 | N41D (AAC→GAC) | hslU | molecular chaperone and ATPase component of HslUV protease |
| Reads supporting (aligned to +/- strand): ref base A (23/17); new base G (2/2); total (25/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.00e-01 | |||||||||||
ATCGGCCAGGACAACGCCAAGCGTTCTGTGGCGATTGCTCTGCGTAACCGCTGGCGTCGCATGCAGCTCAACGAAGAGCTGCGCCATGAAGTGACCCCGAAAAATATCCTGATGATCGGCCCGACCGGTGTCGGTAAAAC > W3110S.gb/3514985‑3515124 | atcGGCCAGGACAACGCCAAGCGTTCTGTGGCGATTGCTCTGCGTAACCGCTGGCGTCGCATGCAGCTCaa < 1:3360862/71‑1 (MQ=255)atcGGCCAGGACAACGCCAAGCGTTCTGTGGCGATTGCTCTGCGTAACCGCTGGCGTCGCATGCAGCTCaa > 1:953748/1‑71 (MQ=255) ggCCAGGACAACGCCAAGCGTTCTGTGGCGATTGCTCTGCGTAACCGCTGGCGTCGCATGCAGCTCAACg > 1:3473388/1‑70 (MQ=255) ggCCAGGACAACGCCAAGCGTTCTGTGGCGATTGCTCTGCGTAACCGCTGGCGTCGCATGCAGCTCAACg < 1:2302520/70‑1 (MQ=255) gCCAGGACAACGCCAAGCGTTCTGTGGCGATTGCTCTGCGTAACCGCTGGCGTCGCATGCAGCTCAACg < 1:2617679/69‑1 (MQ=255) ccAGGACAACGCCAAGCGTTCTGTGGCGATTGCTCTGCGTAACCGCTGGCGTCGCATGCAGCTCAACGAag < 1:3716935/71‑1 (MQ=255) aaCGCCAAGCGTTCTGTGGCGATTGCTCTGCGTAACCGCTGGCGTCGCATGCAGCTCAACGAaga < 1:48926/65‑1 (MQ=255) cGCCAAGCGTTCTGTGGCGATTGCTCTGCGTAACCGCTGGCGTCGCATGCAGCTCAACGAAGAGc > 1:1473086/1‑65 (MQ=255) gCCAAGCGTTCTGTGGCGATTGCTCTGCGTAACCGCTGGCGTCGCATGCAGCTCAACGAAGAGCTGCGCCa > 1:2615924/1‑71 (MQ=255) cAAGCGTTCTGTGGCGATTGCTCTGCGTAACCGCTGGCGTCGCATGCAGCTCGACGAAGAGCTGCGCCATg < 1:3148397/71‑1 (MQ=255) cAAGCGTTCTGTGGCGATTGCTCTGCGTAACCGCTGGCGTCGCATGCAGCTCGACGAAGAGCTGCGCCATg < 1:4492960/71‑1 (MQ=255) cAAGCGTTCTGTGGCGATTGCTCTGCGTAACCGCTGGCGTCGCATGCAGCTCAACGAAGAGCTGCGCCAt > 1:1291851/1‑70 (MQ=255) aGCGTTCTGTGGCGATTGCTCTGCGTAACCGCTGGCGTCGCATGCAGCTCAACGAAGAGCTgcgc < 1:4439478/65‑1 (MQ=255) cTGTGGCGATTGCTCTGCGTAACCGCTGGCGTCGCATGCAGCTCAACGAagag > 1:1601963/1‑53 (MQ=255) cTGTGGCGATTGCTCTGCGTAACCGCTGGCGTCGCATGCAGCTCAACGAAGAGCTgcg > 1:1441375/1‑58 (MQ=255) cTGTGGCGATTGCTCTGCGTAACCGCTGGCGTCGCATGCAGCTCAACGAAGAGCTGCGCCATGAAGTGAcc > 1:3467744/1‑71 (MQ=255) cTGTGGCGATTGCTCTGCGTAACCGCTGGCGTCGCATGCAGCTCAACGAAGAGCTGCGCCATGAAGTGAcc > 1:1984207/1‑71 (MQ=255) gtgGCGATTGCTCTGCGTAACCGCTGGCGTCGCATGCAGCTCAACGaa > 1:2401495/1‑48 (MQ=255) tGCTCTGCGTAACCGCTGGCGTCGCATGCAGCTCAACGAAGAGCTGCGCCATGa > 1:769613/1‑54 (MQ=255) tGCTCTGCGTAACCGCTGGCGTCGCATGCAGCTCAACGAAGAGCTGCGCCATGa > 1:3319018/1‑54 (MQ=255) gCTCTGCGTAACCGCTGGCGTCGCATGCAGCTCAACGaa > 1:4184079/1‑39 (MQ=255) gCTCTGCGTAACCGCTGGCGTCGCATGCAGCTCAACGaa > 1:1030120/1‑39 (MQ=255) gCTCTGCGTAACCGCTGGCGTCGCATGCAGCTCAACGAAGAGCTGCGCCATGAAGTGACCCCGAAAAatat > 1:2140393/1‑71 (MQ=255) gCTCTGCGTAACCGCTGGCGTCGCATGCAGCTCAACGAAGAGCTGCGCCATGAAGTGACCCCGAAAAatat > 1:3508451/1‑71 (MQ=255) gCTCTGCGTAACCGCTGGCGTCGCATGCAGCTCAACGAAGAGCTGCGCCATGAAGTGACCCCGAAAAatat > 1:3813454/1‑71 (MQ=255) tctGCGTAACCGCTGGCGTCGCATGCAGCTCGACGAAGAGCTGCGCCATGaa > 1:3880025/1‑52 (MQ=255) tctGCGTAACCGCTGGCGTCGCATGCAGCTCGACGAAGAGCTGCGCCATGaa > 1:4172248/1‑52 (MQ=255) aCCGCTGGCGTCGCATGCAGCGCAACGAAGAGCTGCGCCATGAAGTGACCCCGAAAAATATCCTgatgat < 1:3771959/70‑1 (MQ=255) cGCTGGCGTCGCATGCAGCTCAACGAAGAGCTGCGCCATGAAGTGACCCCGaaaaa < 1:981026/56‑1 (MQ=255) cTGGCGTCGCATGCAGCTCAACGAAGAGCTGCGCCATGAAGTGACCCCGAAAAATATCCTGATGATCGGc < 1:2944740/70‑1 (MQ=255) gCGTCGCATGCAGCTCAACGAAGAGCTGCGCCATGAAGTGACCCCGAAAAATATCCTgatgat > 1:2967554/1‑63 (MQ=255) cGCATGCAGCTCAACGAAGAGCTGCGCCATGAAGTGACCCCGAAAAATATCCTGATGATCGGCCCGACCg > 1:3905993/1‑70 (MQ=255) gCATGCAGCTCAACGAAGAGCTGCGCCATGAAGTGACCCCGAAAAATATCCTGATGATCGGCCCGACCgg < 1:2766729/70‑1 (MQ=255) tGCAGCTCAACGAAGAGCTGCGCCATGAAGTGACCCCGAAAAATATCCTGATGATCGGCCCGACCGGTGTc < 1:1869873/71‑1 (MQ=255) cAGCTCAACGAAGAGCTGCGCCATGAAGTGACCCCGAAAAATATCCTGATGATCGGCCCGACCGGTGTCgg > 1:2177749/1‑71 (MQ=255) aGCTCAACGAAGAGCTGCGCCATGAAGTGACCCCGAAAAATATCCTGATGATCGGCCCGACCGGTGTCgg > 1:2876849/1‑70 (MQ=255) aGCTCAACGAAGAGCTGCGCCATGAAGTGACCCCGAAAAATATCCTGATGATCGGCCCGACCGGTGTCg > 1:4072141/1‑69 (MQ=255) gCTCAACGAAGAGCTGCGCCATGAAGTGACCCCGAAAAATATCCTGATGATCGGCCCGAcc < 1:1859729/61‑1 (MQ=255) cTCAACGAAGAGCTGCGCCATGAAGTGACCCCGAAAAATATCCtgatga < 1:2500801/49‑1 (MQ=255) cTCAACGAAGAGCTGCGCCATGAAGTGACCCCGAAAAATATCCTGATGATCGGCCCGACCGgt < 1:3723008/63‑1 (MQ=255) cTCAACGAAGAGCTGCGCCATGAAGTGACCCCGAAAAATATCCTGATGATCGGCCCGACCGGTGTCGGTaa < 1:3825903/71‑1 (MQ=255) cTCAACGAAGAGCTGCGCCATGAAGTGACCCCGAAAAATATCCTGATGATCGGCCCGACCGGTGTCGGTaa < 1:2210445/71‑1 (MQ=255) tCAACGAAGAGCTGCGCCATGAAGTGACCCCGAAAAATATCCtgatg < 1:2983132/47‑1 (MQ=255) tCAACGAAGAGCTGCGCCATGAAGTGACCCCGAAAAATATCCTGATGATCGGCCCGACCGGTGTCGGTaa < 1:2147638/70‑1 (MQ=255) cAACGAAGAGCTGCGCCATGAAGTGACCCCGAAAAATATCCtga > 1:2035704/1‑44 (MQ=255) cAACGAAGAGCTGCGCCATGAAGTGACCCCGAAAAATATCCTGATGATCgt > 1:3558950/1‑50 (MQ=255) aaCGAAGAGCTGCGCCATGAAGTGACCCCGAAAAATATCCTGATGATCGGCCCGACCGGTGTCGGTAAAAc > 1:2590807/1‑71 (MQ=255) | ATCGGCCAGGACAACGCCAAGCGTTCTGTGGCGATTGCTCTGCGTAACCGCTGGCGTCGCATGCAGCTCAACGAAGAGCTGCGCCATGAAGTGACCCCGAAAAATATCCTGATGATCGGCCCGACCGGTGTCGGTAAAAC > W3110S.gb/3514985‑3515124 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |