| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 199,072 | T→C | 29.0% | V382A (GTC→GCC) | yaeT → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 199,072 | 0 | T | C | 29.0% | 86.3 / 47.6 | 62 | V382A (GTC→GCC) | yaeT | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (25/19); new base C (7/11); total (32/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.66e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01 | |||||||||||
ACCTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACG > W3110S.gb/199002‑199138 | aCCTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGt > 1:472975/1‑71 (MQ=255)aCCTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGt > 1:1427068/1‑71 (MQ=255) cTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTc > 1:3156295/1‑70 (MQ=255) aTGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCa < 1:2530454/67‑1 (MQ=255) gCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGt > 1:3614223/1‑69 (MQ=255) cGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGCCGATCAGGGTAAgg < 1:2765118/71‑1 (MQ=255) gTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAg > 1:2005278/1‑69 (MQ=255) cTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCa > 1:890281/1‑59 (MQ=255) cTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCa > 1:2430247/1‑59 (MQ=255) cTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCg > 1:942655/1‑71 (MQ=255) cTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGCCGATCAGGGTAAGGAGc > 1:2464019/1‑70 (MQ=255) cTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTCGGGAGCGATCTGGTCGATCAGGGTAAGGAGCg < 1:2348755/71‑1 (MQ=255) cGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCa < 1:1283941/56‑1 (MQ=255) cGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCt < 1:4214056/71‑1 (MQ=255) cGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCt < 1:3648347/71‑1 (MQ=255) tCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGa < 1:3059517/71‑1 (MQ=255) tCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGa < 1:1388351/71‑1 (MQ=255) gcgAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTc > 1:4333929/1‑66 (MQ=255) gcgAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTc > 1:3636089/1‑66 (MQ=255) aaaTGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAg > 1:3446491/1‑56 (MQ=255) aaaTGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAg > 1:186365/1‑56 (MQ=255) aaTGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTc > 1:2102378/1‑71 (MQ=255) aaTGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGCCGATCAGGGTAAGGAGCGTCTGAATCGTc < 1:2037728/71‑1 (MQ=255) aaTGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGCCGATCAGGGTAAGGAGCGTCTGAATCGTc < 1:2254940/71‑1 (MQ=255) aTGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCt > 1:1008991/1‑71 (MQ=255) aTGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGCCGATCAGGGTAAGGAGCGTCTGAATCGTCt > 1:1531940/1‑71 (MQ=255) tGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTg < 1:590544/71‑1 (MQ=255) tGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGCCGATCAGGGTAAGGAGCGTCTGAATCGTCTg < 1:3235653/71‑1 (MQ=255) cGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCg < 1:4321888/40‑1 (MQ=255) cGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCg < 1:3318163/40‑1 (MQ=255) cGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTggg > 1:1244701/1‑71 (MQ=255) cGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTggg > 1:3403151/1‑71 (MQ=255) gTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGCCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGc < 1:1262700/71‑1 (MQ=255) tgaGATGGAAGGTGCATGGCTGGGGAGCGATCTGGCCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGc < 1:3591036/68‑1 (MQ=255) tCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCg < 1:4407868/38‑1 (MQ=255) tCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGCCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGc < 1:3585675/70‑1 (MQ=255) cAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGctt > 1:1025142/1‑71 (MQ=255) aTGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGAt < 1:3822351/36‑1 (MQ=255) ggAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCa > 1:2022628/1‑36 (MQ=255) ggAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCa > 1:4079567/1‑36 (MQ=255) ggAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCttt > 1:4176555/1‑70 (MQ=255) aGGTGCATGGCTGGGGAGCGATCTGGCCGATCAGGGTAAGGAGCGTCTGa < 1:4459048/50‑1 (MQ=255) aGGTGCATGGCTGGGGAGCGATCTGGCCGATCAGGGTAAGGAGCGTCTGa < 1:183618/50‑1 (MQ=255) aGGTGCATGGCTGGGGAGCGATCTGGCCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGaaa > 1:2783680/1‑71 (MQ=255) ggTGCATGGCTGGGGAGCGATCTGGCCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGa < 1:3461335/68‑1 (MQ=255) gTGCATGGCTGGGGAGCGATCTGGCCGATCAGGGTAAGGAGCGTCTGAAt > 1:2167488/1‑50 (MQ=255) cATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGc < 1:1867036/39‑1 (MQ=255) cATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTc < 1:1621538/71‑1 (MQ=255) cATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTc < 1:2116788/71‑1 (MQ=255) cATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTc < 1:677005/71‑1 (MQ=255) cATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTc < 1:2759689/71‑1 (MQ=255) cATGGCTGGGGAGCGATCTGGCCGATCAGGGTAAg > 1:1242353/1‑35 (MQ=255) cATGGCTGGGGAGCGATCTGGCCGATCAGGGTAAg > 1:4617592/1‑35 (MQ=255) gCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGcttct > 1:1597958/1‑56 (MQ=255) gCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTcgata > 1:746903/1‑71 (MQ=255) gCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTcgata > 1:474534/1‑71 (MQ=255) cTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGt > 1:1111894/1‑45 (MQ=255) gggAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTcgatacc < 1:4489263/69‑1 (MQ=255) gAGCGATCTGGCCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTcgataccgata > 1:2219019/1‑71 (MQ=255) gATCTGGCCGATCAGGGTAAGGAGCGTCTGAATCGTc < 1:840527/37‑1 (MQ=255) aTCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGcttc > 1:1144521/1‑44 (MQ=255) cTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACg > 1:2952066/1‑71 (MQ=255) tGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGcttc > 1:4573196/1‑41 (MQ=255) gTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCa < 1:4587355/65‑1 (MQ=255) | ACCTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACG > W3110S.gb/199002‑199138 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |