| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,538,437 | G→A | 16.9% | A170V (GCA→GTA) | rhaS ← | DNA‑binding transcriptional activator, L‑rhamnose‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,538,437 | 0 | G | A | 16.9% | 143.9 / 20.5 | 59 | A170V (GCA→GTA) | rhaS | DNA‑binding transcriptional activator, L‑rhamnose‑binding |
| Reads supporting (aligned to +/- strand): ref base G (26/23); new base A (8/2); total (34/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.66e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01 | |||||||||||
GCATCCCAATTCACCTCATCGGCAAAATGGTCCTCCAGCCAGGCCAGAAGCAAGTTGAGACGTGATGCGCTGTTTTCCAGGTTCTCCTGCAAACTGCTTTTACGCAGCAAGAGCAGTAATTGCATAAACAAGATC > W3110S.gb/3538371‑3538505 | gCATCCCAATTCACCTCATCGGCAAAATGGTCCTCCAGCCAGGCCAGAAGCAAGTTGAGACGTGATGCGCt < 1:3201094/71‑1 (MQ=255) cATCCCAATTCACCTCATCGGCAAAATGGTCCTCCAGCCAGGCCAGAAGCAAGTTGAGACGTGATGCGCTg < 1:44603/71‑1 (MQ=255) cATCCCAATTCACCTCATCGGCAAAATGGTCCTCCAGCCAGGCCAGAAGCAAGTTGAGACGTGATGCGCTg < 1:1450558/71‑1 (MQ=255) tCCCAATTCACCTCATCGGCAAAATGGTCCTCCAGCCAGGCCAGAAGCAAGTTGAGACGTGATGCGCTGtt < 1:3211784/71‑1 (MQ=255) aaTTCACCTCATCGGCAAAATGGTCCTCCAGCCAGGCCAGAAGCAAGTTGAGACGTGATGCGCTGTTTTcc < 1:981194/71‑1 (MQ=255) aaTTCACCTCATCGGCAAAATGGTCCTCCAGCCAGGCCAGAAGCAAGTTGAGACGTGATGCGCTGTTTTcc < 1:810372/71‑1 (MQ=255) aTTCACCTCATCGGCAAAATGGTCGTCCAGCCAGGCCAGAAGCAAGTTGAGACGTGATGCGCTGtttt > 1:2535232/1‑68 (MQ=255) ttCACCTCATCGGCAAAATGGTCCTCCAGCCAGGCCAGAAGCAAGTTGAGACGTGATGCGCTGTTTTCCAg > 1:3206824/1‑71 (MQ=255) cTCATCGGCAAAATGGTCCTCCAGCCAGGCCAGAAGCAAGTTGAGACGTGATGCGCTGTTTTCCAGGTtct > 1:3565639/1‑71 (MQ=255) tCATCGGCAAAATGGTCCTCCAGCCAGGCCAGAAGCAAGTTGAGACGTGATGCGCt < 1:2420112/56‑1 (MQ=255) cATCGGCAAAATGGTCCTCCAGCCAGGCCAGAAGCAAGTTGAGACGTGATGCGCTGTTTTCCAGGTTCTcc > 1:1484453/1‑71 (MQ=255) cATCGGCAAAATGGTCCTCCAGCCAGGCCAGAAGCAAGTTGAGACGTGATACGCTGTTTTCCAGGTTCTcc > 1:4536053/1‑71 (MQ=255) ggCAAAATGGTCCTCCAGCCAGGCCAGAAGCAAGTTGAGACGTGATGCGCTGTTTTCCAGGTTCTCCTGc > 1:4485175/1‑70 (MQ=255) ggCAAAATGGTCCTCCAGCCAGGCCAGAAGCAAGTTGAGACGTGATGCGCTGTTTTCCAGGTTCTCCTGc > 1:4206095/1‑70 (MQ=255) ggCAAAATGGTCCTCCAGCCAGGCCAGAAGCAAGTTGAGACGTGATACGCTGTTTTCCAGGTTCTCCt > 1:1120675/1‑68 (MQ=255) ggCAAAATGGTCCTCCAGCCAGGCCAGAAGCAACTTGAGACGTGATGCGCTGt < 1:615585/53‑1 (MQ=255) gCAAAATGGTCCTCCAGCCAGGCCAGAAGCAAGTTGAGACGTGATGCGCTGTTTTCCAGGTTCTCCTGCa < 1:2814191/70‑1 (MQ=255) cAAAATGGTCCTCCAGCCAGGCCAGAAGCAAGTTGAGACGTGATGCGCTGttt > 1:1109377/1‑53 (MQ=255) aaaTGGTCCTCCAGCCAGGCCAGAAGCAAGTTGAGACGTGATGCGCTGTTTTCCAg > 1:924/1‑56 (MQ=255) aaaTGGTCCTCCAGCCAGGCCAGAAGCAAGTTGAGACGTGATGCGCTGTTTTCCAGGTTCTCCTGCAAACt < 1:3648851/71‑1 (MQ=255) aaaTGGTCCTCCAGCCAGGCCAGAAGCAAGTTGAGACGTGATGCGCTGTTTTCCAGGTTCTCCTGCAAACt > 1:4055402/1‑71 (MQ=255) aaTGGTCCTCCAGCCAGGCCAGAAGCAAGTTGAGACGTGATGCGCTGTTTTCCAGGTTCTCCTGCAAAATg > 1:580913/1‑71 (MQ=255) tGGTCCTCCAGCCAGGCCAGAAGCAAGTTGAGACGTGATGCGCTGTTTTCCAGGTtct < 1:4045020/58‑1 (MQ=255) tGGTCCTCCAGCCAGGCCAGAAGCAAGTTGAGACGTGATACGCTGtt > 1:659306/1‑47 (MQ=255) tGGTCCTCCAGCCAGGCCAGAAGCAAGTTGAGACGTGATACGCTGtt > 1:2847610/1‑47 (MQ=255) ggTCCTCCAGCCAGGCCAGAAGCAAGTTGAGACGTGATGCGCTGTTTTcc > 1:1808997/1‑50 (MQ=255) gTCCTCCAGCCAGGCCAGAAGCAAGTTGAGACGTGATGCGCTGTTTTCCa > 1:2485765/1‑50 (MQ=255) gTCCTCCAGCCAGGCCAGAAGCAAGTTGAGACGTGATGCGCTGTTTTCCAGGTTCTCCTGCAAACTGCttt < 1:2872985/71‑1 (MQ=255) tcctccAGCCAGGCCAGAAGCAAGTTGAGACGTGATGCGCTGTTTTCCAGGTTCTCCTGCAAACTGCtttt < 1:428897/71‑1 (MQ=255) ctccAGCCAGGCCAGAAGCAAGTTGAGACGTGATGCGCTg < 1:1568350/40‑1 (MQ=255) ctccAGCCAGGCCAGAAGCAAGTTGAGACGTGATACGCTGTTTTCCAGGTTCTCCTGCAAACTg < 1:68759/64‑1 (MQ=255) tccAGCCAGGCCAGAAGCAAGTTGAGACGTGATGCGCTGTTTTCCAGGTTCTCCTGCa < 1:3645738/58‑1 (MQ=255) ccagccagGCCAGAAGCAAGTTGAGACGTGATGCGCTGTTTTCCAGGTTCTCCTGCAAACTGCTTTTa > 1:1623616/1‑68 (MQ=255) ccagccagGCCAGAAGCAAGTTGAGACGTGATGCGCTGTTTTCCAGGTTCTCCTGCAAACTGCTTTTACg > 1:2277075/1‑70 (MQ=255) ccagccagGCCAGAAGCAAGTTGAGACGTGATACGCTGTTTTCCAGGTTCTCCTGCAAACTGCt > 1:2686230/1‑64 (MQ=255) cagccagGCCAGAAGCAAGTTGAGACGTGATGCGCTGTTTTCCAGGTTCTCCTGCaaa > 1:4031323/1‑58 (MQ=255) gAAGCAAGTTGAGACGTGATGCGCTGTTTT‑CAGGTTCTCCTGCAAACTGCTTTTACGCAGCAAGAGCAGTa < 1:2131123/71‑1 (MQ=255) gAAGCAAGTTGAGACGTGATACGCTGTTTTCCAGGTTCTCCTGCAAACTGCTTTTACGCAGCAagag > 1:1015349/1‑67 (MQ=255) aaGCAAGTTGAGACGTGATGCGCTGTTTTCCAGGTTCTCCTGCAAACTGc < 1:2736049/50‑1 (MQ=255) aaGCAAGTTGAGACGTGATACGCTGTTTTCCAGGTTCTCCTGCAAACTGCTTTTACGCAGCAAGAGCAGt > 1:2084628/1‑70 (MQ=255) aGCAAGTTGAGACGTGATGCGCTGTTTTCCAGGTtctc > 1:2594608/1‑38 (MQ=255) aGCAAGTTGAGACGTGATGCGCTGTTTTCCAGGTTCTCCTGCAAACTGCTTTTACGCAGCAaga > 1:1130789/1‑64 (MQ=255) aGCAAGTTGAGACGTGATGCGCTGTTTTCCAGGTTCTCCTGCAAACTGCTTTTACGCAGCAAGAGCAGTaa > 1:2013397/1‑71 (MQ=255) aGCAAGTTGAGACGTGATGCGCTGTTTTCCAGGTTCTCCTGCAAACTGCTTTTACGCAGCAAGAGCAGTa < 1:663020/70‑1 (MQ=255) gCAAGTTGAGACGTGATACGCTGTTTTCCAGGTTCTCCTGCAAACTGCTTTTACGCAGCAAGAGCAGTAAt > 1:2911338/1‑71 (MQ=255) aaGTTGAGACGTGATGCGCTGTTTTCCAGGTTCTCCTGCa < 1:1291831/40‑1 (MQ=255) gTTGAGACGTGATGCGCTGTTTTCCAGGTTCTCCTGCAAACTGCtttt < 1:4255757/48‑1 (MQ=255) gTTGAGACGTGATGCGCTGTTTTCCAGGTTCTCCTGCAAACTGCtttt < 1:2977815/48‑1 (MQ=255) ttGAGACGTGATGCGCTGTTTTCCAGGTTCTCCTGCAAACTGCTTTTACGCAGCAAGAGCAGTAATTGCa > 1:209872/1‑70 (MQ=255) gagaCGTGATGCGCTGTTTTCCAGGTTCTCCTGCAAACTGCTTTTACGCAGCaa > 1:1242068/1‑54 (MQ=255) gagaCGTGATGCGCTGTTTTCCAGGTTCTCCTGCAAACTGCTTTTACGCAGCAAGAGCAGTaa > 1:3912396/1‑63 (MQ=255) gTGATACGCTGTTTTCCAGGTTCTCCTGCAAACTGCTTTTACGCAGCAAGAGCAGTAATTGCATAAACAAg < 1:1651071/71‑1 (MQ=255) tGATGCGCTGTTTTCCAGGTTCTCCTGCAAACTGCTTTTACGCAGCAAGAGCa > 1:429108/1‑53 (MQ=255) tGATGCGCTGTTTTCCAGGTTCTCCTGCAAACTGCTTTTACGCAGCAAGAGCa > 1:2466585/1‑53 (MQ=255) tGATGCGCTGTTTTCCAGGTTCTCCTGCAAACTGCTTTTACGCAGCAAGAGCAGTAATTGCATAAACAAg < 1:3939980/70‑1 (MQ=255) aTGCGCTGTTTTCCAGGTTCTCCTGCAAACTGCtttt > 1:1784638/1‑37 (MQ=255) aTGCGCTGTTTTCCAGGTTCTCCTGCAAACTGCTTTTACGCAGCAAGAGCAGTAATTGCATAAACAAGAtc > 1:535889/1‑71 (MQ=255) aTGCGCTGTTTTCCAGGTTCTCCTGCAAACTGCTTTTACGCAGCAAGAGCAGTAATTGCATAAACAAGAtc < 1:3461196/71‑1 (MQ=255) tGCGCTGTTTTCCAGGTTCTCCTGCAAACTGCTTTTACGCAGCAAGAGCa > 1:4542387/1‑50 (MQ=255) gcgcTGTTTTCCAGGTTCTCCTGCAAACTGCTTTTACGCAGCAAGAGCAGt < 1:1223242/51‑1 (MQ=255) gcgcTGTTTTCCAGGTTCTCCTGCAAACTGCTTTTACGCAGCAAGAGCAGt < 1:3031990/51‑1 (MQ=255) | GCATCCCAATTCACCTCATCGGCAAAATGGTCCTCCAGCCAGGCCAGAAGCAAGTTGAGACGTGATGCGCTGTTTTCCAGGTTCTCCTGCAAACTGCTTTTACGCAGCAAGAGCAGTAATTGCATAAACAAGATC > W3110S.gb/3538371‑3538505 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |