| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,560,351 | G→A | 13.8% | P62L (CCC→CTC) | rbn ← | tRNA processing exoribonuclease BN |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,560,351 | 0 | G | A | 13.8% | 236.7 / 24.9 | 87 | P62L (CCC→CTC) | rbn | tRNA processing exoribonuclease BN |
| Reads supporting (aligned to +/- strand): ref base G (36/39); new base A (6/6); total (42/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
ATCGCCAGTAGCAGGCAGAAAGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCAACGACACATAGGCA > W3110S.gb/3560281‑3560419 | aTCGCCAGTAGCAGGCAGAAAGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATgg < 1:1360058/71‑1 (MQ=255) gCCAGTAGCAGGCAGAAAGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGaa < 1:1203778/71‑1 (MQ=255) ccAGTAGCAGGCAGAAAGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGaaa < 1:2088149/71‑1 (MQ=255) gTAGCAGGCAGAAAGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAgc > 1:4078748/1‑71 (MQ=255) tAGCAGGCAGAAAGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAgcg > 1:3024132/1‑71 (MQ=255) aGCAGGCAGAAAGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAgcgg > 1:4296760/1‑71 (MQ=255) gcaggcagAAAGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAgcggc > 1:3149211/1‑71 (MQ=255) gcaggcagAAAGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGAGAAAAgcggc > 1:2359394/1‑71 (MQ=255) caggcagAAAGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGAGAAAAgcggc > 1:3102227/1‑70 (MQ=255) gcagAAAGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGaaa < 1:2891408/71‑1 (MQ=255) gcagAAAGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGaa > 1:1915708/1‑70 (MQ=255) agAAAGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAg < 1:415541/71‑1 (MQ=255) gAAAGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGaaaa < 1:1215783/69‑1 (MQ=255) gAAAGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGaaaa < 1:132981/69‑1 (MQ=255) gAAAGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAgc < 1:46592/71‑1 (MQ=255) aaGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGAGAAAAGCGGCGAAAAgcgc < 1:1842423/71‑1 (MQ=255) aGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAgcgc > 1:2148275/1‑70 (MQ=255) aGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAgcgc > 1:107694/1‑70 (MQ=255) aGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAgcgc > 1:1192809/1‑70 (MQ=255) aGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAgcg > 1:1206680/1‑69 (MQ=255) aGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCa < 1:2587143/71‑1 (MQ=255) gTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGAGAAAAGCGGCGAAAAGCGCa > 1:4213495/1‑70 (MQ=255) tagCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGAGAAAAGCGGCGAAAAGCGCaaa > 1:2031758/3‑70 (MQ=255) ggCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAgc > 1:2597848/1‑64 (MQ=255) gCAAAAATAAAGTGACTTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAaac < 1:1841551/71‑1 (MQ=255) gCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGAGAAAAGCGGCGAAAAGCGCaaaaa > 1:4266277/1‑70 (MQ=255) cAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAaac < 1:2316131/70‑1 (MQ=255) aaaaaTAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAaaca > 1:3288034/1‑70 (MQ=255) aaaaaTAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGCCGAAAAGCGCaa < 1:454232/65‑1 (MQ=255) aaaaaTAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGAGAAAAGCGGCGAAAAGCGCaaa < 1:2209085/66‑1 (MQ=255) aaaaTAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAaacaac > 1:3230344/1‑71 (MQ=255) aaTAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAaacaa > 1:2898763/1‑68 (MQ=255) aaTAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAaacaa > 1:2585452/1‑68 (MQ=255) aTAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGc < 1:993620/71‑1 (MQ=255) tAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCa < 1:2140627/71‑1 (MQ=255) gTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAaac < 1:4149654/60‑1 (MQ=255) gTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAAc > 1:199241/1‑69 (MQ=255) gTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCa < 1:1897750/71‑1 (MQ=255) gTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCa < 1:4522374/71‑1 (MQ=255) gACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAaacaa > 1:147705/1‑60 (MQ=255) cGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCgg < 1:285704/71‑1 (MQ=255) cGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCgg < 1:1340431/71‑1 (MQ=255) gTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCgg > 1:923935/1‑70 (MQ=255) gTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCgg > 1:600965/1‑70 (MQ=255) tAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCgg < 1:4520226/69‑1 (MQ=255) aaCTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCAc > 1:2145732/1‑71 (MQ=255) aaCTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCAc > 1:3590098/1‑71 (MQ=255) aCTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGc > 1:4111605/1‑68 (MQ=255) aCTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCAc > 1:2864967/1‑70 (MQ=255) aCTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACt < 1:243101/71‑1 (MQ=255) aCTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACt < 1:1735397/71‑1 (MQ=255) aCTGAATGCTGACGTCGGAAAACATGAGAAAAGCGGCGAAAAgc > 1:756977/1‑44 (MQ=255) cTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAaac < 1:2704829/51‑1 (MQ=255) gAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCa < 1:1583788/56‑1 (MQ=255) aaTGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGTCACTAAt < 1:4468560/70‑1 (MQ=255) aaTGCTGACGTCGGAAAACATGAGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATg < 1:3645413/71‑1 (MQ=255) aaTGCTGACGTCGGAAAACATCGGAAAAGCTGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAAt > 1:876562/1‑70 (MQ=255) aTGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCaa > 1:1248821/1‑55 (MQ=255) aTGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAAt > 1:686063/1‑69 (MQ=255) aTGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAAt > 1:1934939/1‑69 (MQ=255) tGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCaa < 1:1019566/54‑1 (MQ=255) tGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAAt > 1:647038/1‑68 (MQ=255) ggtGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATga > 1:393725/3‑69 (MQ=255) cTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGagag > 1:2952578/1‑71 (MQ=255) tGACGTAGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGc < 1:2114740/49‑1 (MQ=255) aCGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGc < 1:4337281/69‑1 (MQ=255) aCGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGc < 1:1536593/69‑1 (MQ=255) aCGTCGGAAAACATGAGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCa < 1:581376/70‑1 (MQ=255) aCGTCGGAAAACATGAGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCa < 1:3034653/70‑1 (MQ=255) cGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCAAc < 1:2453132/71‑1 (MQ=255) cGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCAAc < 1:3088045/71‑1 (MQ=255) tCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAaac < 1:682442/38‑1 (MQ=255) tCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAaac < 1:4327433/38‑1 (MQ=255) ggAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATga > 1:3925909/1‑60 (MQ=255) ggAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATg > 1:1804562/1‑59 (MQ=255) ggAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCaa > 1:685232/1‑66 (MQ=255) ggAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCAACGaca > 1:3754626/1‑71 (MQ=255) ggAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCAACGac < 1:130633/70‑1 (MQ=255) ggAAAACATGAGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCAACGaca < 1:2526232/71‑1 (MQ=255) aaaaCATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCAACGAcaca < 1:1891524/71‑1 (MQ=255) aaaaCATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCAACGAcaca < 1:2848794/71‑1 (MQ=255) aaaCATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAAc < 1:3605002/42‑1 (MQ=255) aaaCATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCAACGACACAt < 1:1775403/71‑1 (MQ=255) aaCATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGc < 1:4050954/60‑1 (MQ=255) cATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTTATGAGAGCAACGACACATAgg > 1:3676448/1‑71 (MQ=255) cATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCAACGACACATAgg > 1:3264713/1‑71 (MQ=255) aTGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCa > 1:1131085/1‑46 (MQ=255) tGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCAACGACACATAGGCa < 1:2998344/71‑1 (MQ=255) | ATCGCCAGTAGCAGGCAGAAAGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCAACGACACATAGGCA > W3110S.gb/3560281‑3560419 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |