| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,560,998 | A→G | 29.8% | F44S (TTT→TCT) | yihX ← | predicted hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,560,998 | 0 | A | G | 29.8% | 60.6 / 36.6 | 47 | F44S (TTT→TCT) | yihX | predicted hydrolase |
| Reads supporting (aligned to +/- strand): ref base A (19/14); new base G (7/7); total (26/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.52e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01 | |||||||||||
TAGAGCCATCTCATGACACAGCGCCTCTGCGAACGCTTCGTCGCTAATTTCCCCACGCTCATGCTGATGAAACGCCTCCCCCATATGAAAACTCTTCTTAAGCGATGCCAGCGGAATACGCGTTAAATCGCTCCA > W3110S.gb/3560928‑3561062 | tAGAGCCATCTCATGACACAGCGCCTCTGCGAACGCTTCGTCGCTAATTTCCCCACGCTCATGCTGATGaa < 1:3732193/71‑1 (MQ=255) agagCCATCTCATGACACAGCGCCTCTGCGAACGCTTCGTCGCTAATTTCCCCACGCTCATGCTGATGaaa > 1:3401180/1‑71 (MQ=255) gagCCATCTCATGACACAGCGCCTCTGCGAACGCTTCTTCGCTAATTTCCCCACGCTCATGCTGATGAGAc < 1:1877925/71‑1 (MQ=255) gCCATCTCATGACACAGCGCCTCTGCGAACGCTTCGTCGCTAATTTCCCCACGCTCATGCTGATGAAACGc > 1:1585068/1‑71 (MQ=255) tctcATGACACAGCGCCTCTGCGAACGCTTCGTCGCTAATTTCCCCACGCTCATGCTGATGAAACGCCTcc > 1:2451014/1‑71 (MQ=255) tcATGACACAGCGCCTCTGCGAACGCTTCGTCGCTAATTTCCCCACGCTCATGCTGATGAAACGcc < 1:884666/66‑1 (MQ=255) aTGACACAGCGCCTCTGCGAACGCTTCGTCGCTAATTTCCCCACGCTCATGCTGATGaaa > 1:753050/1‑60 (MQ=255) aTGACACAGCGCCTCTGCGAACGCTTCGTCGCTAATTTCCCCACGCTCATGCTGATGAAACGCCTc > 1:4629261/1‑66 (MQ=255) cacaGCGCCTCTGCGAACGCTTCGTCGCTAATTTCCCCACGCTCATGCTGATGAAACGCCTCCCCCATATg < 1:2154390/71‑1 (MQ=255) acaGCGCCTCTGCGAACGCTTCGTCGCTAATTTCCCCACGCTCATGCTGATGAAACGCCTCCCCCATATg < 1:1847470/70‑1 (MQ=255) caGCGCCTCTGCGAACGCTTCGTCGCTAATTTCCCCACGCTCATGCTGATGAAACGc < 1:2479069/57‑1 (MQ=255) aGCGCCTCTGCGAACGCTTCGTCGCTAATTTCCCCACGCTCATGCTGATGAGACGCCTCCCCCATATGaa > 1:1375062/1‑70 (MQ=255) cgcCTCTGCGAACGCTTCGTCGCTAATTTCCCCACGCTCATGCTGATGAGACGCCTCCCCCATATGaaa > 1:545137/1‑69 (MQ=255) tGCGAACGCTTCGTCGCTAATTTCCCCACGCTCATGCTGATGaaa > 1:910965/1‑45 (MQ=255) tGCGAACGCTTCGTCGCTAATTTCCCCACGCTCATGCTGATGAAACGCCTCCCCCATATGaaaa > 1:3332346/1‑64 (MQ=255) tGCGAACGCTTCGTCGCTAATTTCCCCACGCTCATGCTGATGAAACGCCTCCCCCATATGaa < 1:1694401/62‑1 (MQ=255) gCGAACGCTTCGTCGCTAATTTCCCCACGCTCATGCTGATGAAAc > 1:3705694/1‑45 (MQ=255) gCGAACGCTTCGTCGCTAATTTCCCCACGCTCATGCTGATGAAAc > 1:1514316/1‑45 (MQ=255) cGAACGCTTCGTCGCTAATTTCCCCACGCTCATGCTGATGAGACGCCTCCCCCATATGAAAACTcttctt > 1:3849061/1‑70 (MQ=255) cGCTTCGTCGCTAATTTCCCCACGCTCATGCTGATGAGACGCCTCCCCCATATGAAAACTCTTCTTAAGc > 1:1265027/1‑70 (MQ=255) cGCTTCGTCGCTAATTTCCCCACGCTCATGCTGATGAGACGCCTCCCCCATATGAAAACTCTTCTTAAGc > 1:1090320/1‑70 (MQ=255) gCTTCGTCGCTAATTTCCCCACGCTCATGCTGATGAGAc < 1:4440242/39‑1 (MQ=255) gCTTCGTCGCTAATTTCCCCACCCTCATGCTGATGAAACGCCTCCCCCATATGaa < 1:257919/55‑1 (MQ=255) cTTCGTCGCTAATTTCCCCACGCTCATGCTGATGAAACGCCTCCCCCATATGAAAACTCTTCTTAAGCGAt < 1:1406274/71‑1 (MQ=255) tcgtcgCTAATTTCCCCACGCTCATGCTGATGAAACGCCTCCCCCATATGAAAACTCTTCTTAAGCGATGc > 1:3400036/1‑71 (MQ=255) cgtcgCTAATTTCCCCACGCTCATGCTGATGAGACGCCTCCCCCATATGAAAACTCTTCTTAAGCGATGc > 1:1628048/1‑70 (MQ=255) gtcgCTAATTTCCCCACGCTCATGCTGATGAAACGCCTCCCCCATATGAAAACTCTTCTTAAGCGATGc > 1:1768642/1‑69 (MQ=255) cgCTAATTTCCCCACGCTCATGCTGATGAAACGCCTCCCCCATATGAAAACTCTTCTTAAGCGATGCCAGc > 1:3851937/1‑71 (MQ=255) gCTAATTTCCCCACGCTCATGCTGATGAGACGCCTCCCCCATATGAAAACTCTTCTTAAGCGATGCCAGc < 1:3048111/70‑1 (MQ=255) cTAATTTCCCCACGCTCATGCTGATGAAACGCCTCCCCCATATGAAAACTCTTCTTAAGCGATGCCAGCgg < 1:4147032/71‑1 (MQ=255) cTAATTTCCCCACGCTCATGCTGATGAAACGCCTCCCCCATATGAAAACTCTTCTTAAGCGATGCCAGCgg < 1:884990/71‑1 (MQ=255) aTTTCCCCACGCTCATGCTGATGAAACGCCTCCCCCATATGAAAACTCTTCTTAAGCGATGCCAGCGGaa < 1:916061/70‑1 (MQ=255) aTTTCCCCACGCTCATGCTGATGAAACGCCTCCCCCATATGAAAACTCTTCTTAAGCGATGCCAGCGGa < 1:2016665/69‑1 (MQ=255) tttCCCCACGCTCATGCTGATGAGACGCCTCCCCCATATGAAAACTCTTCTTAAGc < 1:3639909/56‑1 (MQ=255) tttCCCCACGCTCATGCTGATGAAACGCCTCCCCCATATGAAAACTCTTCTTAAGCGATGCCAGCgg < 1:38409/67‑1 (MQ=255) tttCCCCACGCTCATGCTGATGAAACGCCTCCCCCATATGAAAACTCTTCTTAAGCGATGCCAGCgg < 1:2424160/67‑1 (MQ=255) ttCCCCACGCTCATGCTGATGAAACGCCTCCCCCATATGAAAACtcttct > 1:2849044/1‑50 (MQ=255) tCCCCACGCTCATGCTGATGAGACGCCTCCCCCATATGAAAACTCTTCTTAAg > 1:4059446/1‑53 (MQ=255) cccACGCTCATGCTGATGAGACGCCTCCCCCATATGAAAACTCTTCTTAAGCGATGCCAGCGGaa < 1:574140/65‑1 (MQ=255) aCGCTCATGCTGATGAGACGCCTCCCCCATATGAAAACTCTTCTTAAGCGATGCCAGCGGAATACGCGTTa < 1:4514702/71‑1 (MQ=255) aCGCTCATGCTGATGAAACGCCTCCCCCATATGAAAAc > 1:3967362/1‑38 (MQ=255) aCGCTCATGCTGATGAAACGCCTCCCCCATATGAAAACTCTTCTTAAGCGATGCCAGCGGaa > 1:1784545/1‑62 (MQ=255) gCTCATGCTGATGAAACGCCTCCCCCATATGAAAACTCTTCTTAAGCGATGCCAGCGGAATACGCGTTaa < 1:3573876/70‑1 (MQ=255) cTCATGCTGATGAAACGCCTCCCCCATATGAAAACTCTTCTTAAGCGATg > 1:1352104/1‑50 (MQ=255) tCATGCTGATGAAACGCCTCCCCCATATGAAAACTCTTCTTAAGCGATGCCAGCGGAATACGCGTTAAAt > 1:1184854/1‑70 (MQ=255) tCATGCTGATGAAACGCCTCCCCCATATGAAAACTCTTCTTAAGCGATGCCAGCGGAATACGCGTTAAATc > 1:1559610/1‑71 (MQ=255) cATGCTGATGAAACGCCTCCCCCATATGAAAACTCTTCTTAAGCGATGCCAGCGGaa > 1:114740/1‑57 (MQ=255) tGCTGATGAGACGCCTCCCCCATATGAAAACTCTTCTTAAGCGATGCCAGCGGAATACGCGTTAAATCGCt < 1:3554236/71‑1 (MQ=255) gaAACGCCTCCCCCATATGAAAACTCTTCTTAAGCGATGCCAGCGGAATa > 1:1687182/1‑50 (MQ=255) gaAACGCCTCCCCCATATGAAAACTCTTCTTAAGCGATGCCAGCGGAATa > 1:2115446/1‑50 (MQ=255) gaAACGCCTCCCCCATATGAAAACTCTTCTTAAGCGATGCCAGCGGAATACGCGTTAAATCGCTCCa > 1:3034138/1‑67 (MQ=255) aaaCGCCTCCCCCATATGAAAACTCTTCTTAAGCGa > 1:457135/1‑36 (MQ=255) aaaCGCCTCCCCCATATGAAAACTCTTCTTAAGCGATGCCAGCGGAATa > 1:3105830/1‑49 (MQ=255) | TAGAGCCATCTCATGACACAGCGCCTCTGCGAACGCTTCGTCGCTAATTTCCCCACGCTCATGCTGATGAAACGCCTCCCCCATATGAAAACTCTTCTTAAGCGATGCCAGCGGAATACGCGTTAAATCGCTCCA > W3110S.gb/3560928‑3561062 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |