Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 3,569,811 G→A 5.2% V116I (GTC→ATC)  yihP → predicted transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,569,8110GA 5.2% 243.6 / 3.1 77V116I (GTC→ATC) yihPpredicted transporter
Reads supporting (aligned to +/- strand):  ref base G (47/26);  new base A (2/2);  total (49/28)
Fisher's exact test for biased strand distribution p-value = 6.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

AAAGGTAAGTTCCGTCCTTTTATTCTGTATGCGTCATTCCCGGTCACCTTACTGGCGATCGCCAACTTTGTCGGCACACCGTTTGATGTCACCGGTAAAACGGTGATGGCCACTATTCTGTTTATGCTTTACG  >  W3110S.gb/3569742‑3569874
                                                                     |                                                               
aaaGGTAAGTTCCGTCCTTTTATTCTGTATGCGTCATTCCCGGTCACCTTACTGGCGATCGCCAACTTTGt                                                                >  1:120747/1‑71 (MQ=255)
 aaGGTAAGTTCCGTCCTTTTATTCTGTATGCGTCATTCCCGGTCACCTTACTGGCGATCGCCAACTTTGTc                                                               <  1:4447923/71‑1 (MQ=255)
 aaGGTAAGTTCCGTCCTTTTATTATGTATGCGTCATTCCCGGTCACCTTACTGGCGATCGCCAACTTTGTc                                                               <  1:611919/71‑1 (MQ=255)
  aGGTAAGTTCCGTCCTTTTATTCTGTATGCGTCATTCCCGGTCACCTTACTGGCGATCGCCAACTTTGTCg                                                              >  1:1055715/1‑71 (MQ=255)
   ggTAAGTTCCGTCCTTTTATTCTGTATGCGTCATTCCCGGTCACCTTACTGGCGATCGCCAACTTTGTCgg                                                             <  1:2476634/71‑1 (MQ=255)
   ggTAAGTTCCGTCCTTTTATTCTGTATGCGTCATTCCCGGTCACCTTACTGGCGATCGCCAACTTTGTCgg                                                             >  1:2007287/1‑71 (MQ=255)
   ggTAAGTTCCGTCCTTTTATTCTGTATGCGTCATTCCCGGTCACCTTACTGGCGATCGCCAACTTTGTCgg                                                             <  1:730971/71‑1 (MQ=255)
      aaGTTCCGTCCTTTTATTCTGTATGCGTCATTCCCGGTCACCTTACTGGCGATCGCCAACTTTGTCGGca                                                           >  1:1647346/1‑70 (MQ=255)
      aaGTTCCGTCCTTTTATTCTGTATGCGTCATTCCCGGTCACCTTACTGGCGATCGCCAACTATGTCGGcc                                                           >  1:4015780/1‑69 (MQ=255)
       aGTTCCGTCCTTTTATTCTGTATGCGTCATTCCCGGTCACCTTACTGGCGATCGCCAACTTTGTCGGcac                                                          <  1:3024767/70‑1 (MQ=255)
          tCCGTCCTTTTATTCTGTATGCGTCATTCCCGGTCACCTTACTGGCGATCGCCAACTTTGTCGGCACACCg                                                      >  1:1307441/1‑71 (MQ=255)
            cGTCCTTTTATTCTGTATGCGTCATTCCCGGTCACCTTACTGGCGATCGCCAACTTTGTCGGCACACCGt                                                     <  1:1112043/70‑1 (MQ=255)
             gTCCTTTTATTCTGTATGCGTCATTCCCGGTCACCTTACTGGCGATCGCCAACTTTGTCGGCACACCGttt                                                   <  1:4392860/71‑1 (MQ=255)
                cTTTTATTCTGTATGCGTCATTCCCGGTCACCTTACTGGCGATCGCCAACTTTGTCGGCACACCGttt                                                   <  1:1241737/68‑1 (MQ=255)
                   ttATTCTGTATGCGTCATTCCCGGTCACCTTACTGGCGATCGCCAACTTTGTCGGCACACCGTTTGATGt                                              >  1:16405/1‑70 (MQ=255)
                   ttATTCTGTATGCGTCATTCCCGGTCACCTTACTGGCGATCGCCAACTTTGTCGGCACACCGTTTGATGt                                              >  1:4127184/1‑70 (MQ=255)
                    tATTCTGTATGCGTCATTCCCGGTCACCTTACTGGCGATCGCCAACTTTGTCGGCACACCGTTTGATGTc                                             <  1:3888810/70‑1 (MQ=255)
                    tATTCTGTATGCGTCATTCCCGGTCACCTTACTGGCGATCGCCAACTTTGTCGGCACACCGTTTGATGTCa                                            >  1:737665/1‑71 (MQ=255)
                    tATTCTGTATGCGTCATTCCCGGTCACCTTACTGGCGATCGCCAACTTTGTCGGCACACCGTTTGATGTCa                                            >  1:4552205/1‑71 (MQ=255)
                      ttCTGTATGCGTCATTCCCGGTCACCTTACTGGCGATCGCCAACTTTATCGGCACACCGTTTGATGTCAc                                           <  1:4389588/70‑1 (MQ=255)
                        cTGTATGCGTCATTCCCGGTCACCTTACTGGCGATCGCCAACTTTGTCGGCACACCGTTTGATGTCACCgg                                        >  1:3142060/1‑71 (MQ=255)
                         tGTATGCGTCATTCCCGGTCACCTTACTGGCGATCGCCAACTTTGTCGGCACACCGTTTGATGTCACCgg                                        >  1:311116/1‑70 (MQ=255)
                           tATGCGTCATTCCCGGTCACCTTACTGGCGATCGCCAACTTTGTCGGCACACCGTTTGATGTCACCGGTaa                                     <  1:227480/71‑1 (MQ=255)
                             tGCGTCATTCCCGGTCACCTTACTGGCGATCGCCAACTTTGTCGGCACACCGTTTGAt                                                <  1:769415/58‑1 (MQ=255)
                             tGCGTCATTCCCGGTCACCTTACTGGCGATCGCCAACTTTGTCGGCACACCGTTTGAt                                                <  1:2865275/58‑1 (MQ=255)
                             tGCGTCATTCCCGGTCACCTTACTGGCGATCGCCAACTTTGTCGGCACACCGTTTGAt                                                <  1:3050556/58‑1 (MQ=255)
                              gCGTCATTCCCGGTCACCTTACTGGCGATCGCCATCTTTGTCGGCACACCGTTTGAt                                                <  1:4289741/57‑1 (MQ=255)
                                gTCATTCCCGGTCACCTTACTGGCGATCGCCAACTTTATCGGCACACCGTTTGATGTCACCGGTAAAACgg                                >  1:907813/1‑71 (MQ=255)
                                 tCATTCCCGGTCACCTTACTGGCGATCGCCAACTTTGTCGGCACACCGTTTGATGTCACCGGTAAAACGGt                               <  1:2005139/71‑1 (MQ=255)
                                  cATTCCCGGTCACCTTACTGGCGATCGCCAACTTTGTCGGCACACCGTTTGATGTCACCGGTAAAACGGt                               >  1:4283584/1‑70 (MQ=255)
                                  cATTCCCGGTCACCTTACTGGCGATCGCCAACTTTGTCGGCACACCGTTTGATGTCACCGGTAAAACGGt                               >  1:4056327/1‑70 (MQ=255)
                                  cATTCCCGGGCACCTTACTGGCGATCGCCAACTTTGTCGGcac                                                          <  1:473833/43‑1 (MQ=255)
                                   aTTCCCGGTCACCTTACTGGCGATCGCCAACTTTGTCGGCACACCGTTTGATGTCACCGGTAAAAc                                  >  1:3689746/1‑66 (MQ=255)
                                    ttCCCGGTCACCTTACTGGCGATCGCCAACTTTGTCGGCACACCGTTTGATGTCACCGGTAAAACGGt                               <  1:15247/68‑1 (MQ=255)
                                    ttCCCGGTCACCTTACTGGCGATCGCCAACTTTGTCGGCACACCGTTTGATGTCACCGGTAAAACGGTGAt                            >  1:3192149/1‑71 (MQ=255)
                                     tCCCGGTCACCTTACTGGCGATCGCCAACTTTGTCGGGACACCGTTTGa                                                 >  1:2437903/1‑49 (MQ=255)
                                     tCCCGGTCACCTTACTGGCGATCGCCAACTTTGTCGGCACAccat                                                     <  1:2838474/45‑3 (MQ=255)
                                      cccGGTCACCTTACTGGCGATCTCCAACTTTGTCGGCACACCGTTTGATGTCACCGGTAAAACGGTGa                             >  1:2660050/1‑68 (MQ=255)
                                      cccGGTCACCTTACTGGCGATCGCCAACTTTGTCGGCacac                                                        >  1:1364633/1‑41 (MQ=255)
                                      cccGGTCACCTTACTGGCGATCGCCAACTTTGTCGGCACACCGTTTGa                                                 >  1:4599246/1‑48 (MQ=255)
                                      cccGGTCACCTTACTGGCGATCGCCAACTTTGTCGGCACACCGTTTGATGTCACCGGTAAAACGGTGa                             >  1:897597/1‑68 (MQ=255)
                                      cccGGTCACCTTACTGGCGATCGCCAACTTTGTCGGCACACCGTTTGATGTCACCGGTAAAACGGTGATgg                          >  1:2989438/1‑71 (MQ=255)
                                            cACCTTACTGGCGATCGCCAACTTTGTCGGCACACCGTTTGATGTCACCGGTAAAACGGTg                              >  1:105298/1‑61 (MQ=255)
                                            cACCTTACTGGCGATCGCCAACTTTGTCGGCACACCGTTTGATGTCACCGGTAAAACGGTGa                             >  1:2960385/1‑62 (MQ=255)
                                             aCCTTACTGGCGATCGCCAACTTTGTCGGCACACCGTTTGa                                                 <  1:2810784/41‑1 (MQ=255)
                                             aCCTTACTGGCGATCGCCAACTTTGTCGGCACACCGTTTGATGTCACCGGTaaa                                    <  1:1732460/54‑1 (MQ=255)
                                             aCCTTACTGGCGATCGCCAACTTTGTCGGCACACCGTTTGATGTCACCGGTAAAACGGTGa                             >  1:2193771/1‑61 (MQ=255)
                                              ccTTACTGGCGATCGCCAACTTTGTCGGCACACCGTTTGATGTCACCGGTAAAACGGTGa                             <  1:4007369/60‑1 (MQ=255)
                                               cTTACTGGCGATCGCCAACTTTGTCGGCACACCGTTTGATGTCACCGGTAAAACGGTGATGGCCACTAtt                  >  1:3498093/1‑70 (MQ=255)
                                                ttACTGGCGATCGCCAACTTTGTCGGCACACCGTTTGATGt                                              >  1:3288699/1‑41 (MQ=255)
                                                ttACTGGCGATCGCCAACTTTGTCGGCACACCGTTTGATGt                                              >  1:2435905/1‑41 (MQ=255)
                                                ttACTGGCGATCGCCAACTTTGTCGGCACACCGTTTGATGTCACCGGTAAAACGGTGATGGCCACTATTCt                >  1:4616633/1‑71 (MQ=255)
                                                 tACTGGCGATCGCCAACTTTGTCGGCACACCGTTTGATGTCACCGGTAAAACGGTGATGGCCACTATTCTg               >  1:2889752/1‑71 (MQ=255)
                                                 tACTGGCGATCGCCAACTTTGTCGGCACACCGTTTGATGTCACCGGTAAAACGGTGATGGCCACTATTCTg               >  1:2728210/1‑71 (MQ=255)
                                                  aCTGGCGATCGCCAACTTTGTCGGCACACCGTTTGATGTCACCGGTAAAACGGTGATGGCCACTATTCTGt              >  1:1078780/1‑71 (MQ=255)
                                                  aCTGGCGATCGCCAACTTTGTCGGCACACCGTTTGATGTCACCGGTAAAACGGTGATGGCCACTATTCTGt              >  1:3858914/1‑71 (MQ=255)
                                                  aCTGGCGATCGCCAACTTTATCGGCACACCGTTTGATGTCAc                                           >  1:2615644/1‑42 (MQ=255)
                                                   cTGGCGATCGCCAACTTTGTCGGCACACCGTTTGATGTCACCGGTAAAACGGTGa                             >  1:3595513/1‑55 (MQ=255)
                                                   cTGGCGATCGCCAACTTTGTCGGCACACCGTTTGATGTCACCGGTAAAACGGTGATGGCCACTATTCTGtt             >  1:3061963/1‑71 (MQ=255)
                                                      gCGATCGCCAACTTTGTCGGCACACCGTTTGATGt                                              >  1:336813/1‑35 (MQ=255)
                                                      gCGATCGCCAACTTTGTCGGCACACCGTTTGATGTCACCGGTaaaa                                   >  1:3951634/1‑46 (MQ=255)
                                                      gCGATCGCCAACTTTGTCGGCACACCGTTTGATGTCACCGGTaaaa                                   >  1:4573761/1‑46 (MQ=255)
                                                      gCGATCGCCAACTTTGTCGGCACACCGTTTGATGTCACCGGTAAAACGGTGATGGCCACTATTCTGttt            <  1:3651768/69‑1 (MQ=255)
                                                      gCGATCGCCAACTTTGTCGGCACACCGTTTGATGTCACCGGTAAAACGGTGATGGCCACTATTCTGttt            <  1:1898417/69‑1 (MQ=255)
                                                      gCGATCGCCAACTTTGTCGGCACACCGTTTGATGTCACCGGTAAAACGGTGATGGCCACTATTCTGTTTAt          >  1:2279520/1‑71 (MQ=255)
                                                       cGATCGCCAACTTTGTCGGCACACCGTTTGATGTCACCGGTaaaa                                   >  1:369782/1‑45 (MQ=255)
                                                       cGATCGCCAACTTTGTCGGCACACCGTTTGATGTCACCGGTAAAACGGTGa                             >  1:1293020/1‑51 (MQ=255)
                                                         aTCGCCAACTTTATCGGCACACCGTTTGATGTCACCGGTAAAACGGt                               <  1:4574242/47‑1 (MQ=255)
                                                          tCGCCAACTTTGTCGGCACACCGTTTGATGTCACCGGTAAAACGGTGATGGCCACTATTCTGTTTATGCtt      >  1:2160463/1‑71 (MQ=255)
                                                          tCGCCAACTTTGTCGGCACACCGTTTGATGTCACCGGTAAAACGGTGATGGCCACTATTCTGTTTATGCtt      >  1:3995116/1‑71 (MQ=255)
                                                           cGCCAACTTTGTCGGCACACCGTTTGATGTCACCGGTAAAACGGTGATGGCCACt                     >  1:3040439/1‑55 (MQ=255)
                                                           cGCCAACTTTGTCGGCACACCGTTTGATGTCACCGGTAAAACGGTGATGGCCACTATTCTGTTTa           >  1:1577023/1‑65 (MQ=255)
                                                            gCCAACTTTGTCGGCACACCGTTTGATGTCACCGGTAAAACGGTGATGGCCACTATTCTGTTTATGCttt     <  1:2725770/70‑1 (MQ=255)
                                                             ccAACTTTGTCGGCACACCGTTTGATGTCACCGGTAAAACGGTGATGGCCACTAt                   <  1:1409486/55‑1 (MQ=255)
                                                              cAACTTTGTCGGTACACCGTTTGATGTCACCGGTAAAACGGTGATGGCCACTATTCTGTTTATGCTTTACg  <  1:680002/71‑1 (MQ=255)
                                                               aaCTTTGTCGGCACACCGTTTGATGTCACCGGTAAAACGGTGATGGCCACTATTCTGTTTATGCTTTACg  >  1:2330142/1‑70 (MQ=255)
                                                                aCTTTGTCGGCACACCGTTTGATGTCACCGGTAAAACGGTGATGGCCa                       >  1:1663105/1‑48 (MQ=255)
                                                                     gTCGGCACACCGTTTGATGTCACCGGTAAAACGGt                               >  1:348253/1‑35 (MQ=255)
                                                                     |                                                               
AAAGGTAAGTTCCGTCCTTTTATTCTGTATGCGTCATTCCCGGTCACCTTACTGGCGATCGCCAACTTTGTCGGCACACCGTTTGATGTCACCGGTAAAACGGTGATGGCCACTATTCTGTTTATGCTTTACG  >  W3110S.gb/3569742‑3569874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑