| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,608,041 | C→T | 20.0% | L45L (CTG→TTG) | fadA → | 3‑ketoacyl‑CoA thiolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,608,041 | 0 | C | T | 20.0% | 62.3 / 9.8 | 30 | L45L (CTG→TTG) | fadA | 3‑ketoacyl‑CoA thiolase |
| Reads supporting (aligned to +/- strand): ref base C (10/14); new base T (4/2); total (14/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.78e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01 | |||||||||||
TTTCGTAACGTGCGTGCAGAAGATCTCTCCGCTCATTTAATGCGTAGCCTGCTGGCGCGTAACCCGGCGCTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTTGTGTGCAGCAGACGCTGGAGCAGGGTTTT > W3110S.gb/3607972‑3608106 | tttCGTAACGTGCGTGCAGAAGATCTCTCCGCTCATTTAATGCGTAGCCTGCTGGCGCGTAACCCGGCGCt > 1:3015844/1‑71 (MQ=255) tCGTAACGTGCGTGCAGAAGATCTCTCCGCTCATTTAATGCGTAGCCTGCTGGCGCGTAACCCGGCGCTg > 1:2459475/1‑70 (MQ=255) aCGTGCGTGCAGAAGATCTCTCCGCTCATTTAATGCGTAGCCTGCTGGCGCGTAACCCGGCGCTGGAAgc < 1:1061413/70‑1 (MQ=255) gtgcgtgcAGAAGATCTCTCCTCTCATTTAATGCGTAGCCTGCTGGCGCGTAACCCGGCGCTGTAAgcggc < 1:4497519/71‑1 (MQ=255) gcgtgcAGAAGATCTCTCCGCTCATTTAATGCGTAGCCTGCTGGCGCGTAACCCGGCGCTg < 1:2738477/61‑1 (MQ=255) cAGAAGATCTCTCCGCTCATTTAATGCGTAGCCTGCTGGCGCGTAACCCGGCGCTGGAAGCGGCGGCCCTc > 1:283006/1‑71 (MQ=255) agaagaTCTCTCCGCTCATTTAATGCGTAGCCTGCTGGCGCGTAACCCGGCGCt < 1:3561007/54‑1 (MQ=255) agaTCTCTCCGCTCATTTAATGCGTAGCCTGCTGGCGCGTAACCCGGCGCTGGAAGCGGCGGCCCTcgac > 1:2804969/1‑70 (MQ=255) gaTCTCTCCGCTCATTTAATGCGTAGCCTGCTGGCGCGTAACCCGGCGTTGGaa > 1:3868400/1‑54 (MQ=255) gaTCTCTCCGCTCATTTAATGCGTAGCCTGCTGGCGCGTAACCCGGCGCTg > 1:3648978/1‑51 (MQ=255) gaTCTCTCCGCTCATTTAATGCGTAGCCTGCTGGCGCGTAACCCGGCGCTGGAAGCGGCGGCCCt > 1:2494119/1‑65 (MQ=255) tctctcCGCTCATTTAATGCGTAGCCTGCTGGCGCGTAACCCGGCGTTGGa > 1:4011686/1‑51 (MQ=255) ctctcCGCTCATTTAATGCGTAGCCTGCTGGCGCGTAACCCGGCGCTGGAAGCGGCGGCCCTCGACGatat > 1:498301/1‑71 (MQ=255) tcCGCTCATTTAATGCGTAGCCTGCTGGCGCGTAACCCGGCGCTGGAAGCGGCGGCCCTcgac > 1:3893453/1‑63 (MQ=255) tcCGCTCATTTAATGCGTAGCCTGCTGGCGCGTAACCCGGCGCTGGAAGCGGCGGCCCTCGACGata < 1:4245317/67‑1 (MQ=255) tcCGCTCATTTAATGCGTAGCCTGCTGGCGCGTAACCCGGCGCGGGAAGCGGCGGCCCTCGACGat < 1:3116646/66‑1 (MQ=255) cGCTCATTTAATGCGTAGCCTGCTGGCGCGTAACCCGGCGCTGGAAGCGGCGGCCCTCGACGATATTTa < 1:714565/69‑1 (MQ=255) gCTCATTTAATGCGTAGCCTGCTGGCGCGTAACCCGGCGCTGGAAGCGGCGGCCCTCGACGATATTTACTg < 1:4061991/71‑1 (MQ=255) cTCATTTAATGCGTAGCCTGCTGGCGCGTAACCCGGCGTTGGAAGCGGCGGCCCTCGCCGATAATTAc > 1:2331639/1‑68 (MQ=255) tCATTTAATGCGTAGCCTGCTGGCGCGTAACCCGGCGTTGGAAGCGGCGGCCCTCGACGATATTTa > 1:3656793/1‑66 (MQ=255) tttAATGCGTAGCCTGCTGGCGCGTAACCCGGCGCTGGAAGCGGCGGCCCTCGACGata < 1:3564362/59‑1 (MQ=255) ccTGCTGGCGCGTAACCCGGCGTTGGAAGCGGCGGCCCTcgacga < 1:714096/45‑1 (MQ=255) ccTGCTGGCGCGTAACCCGGCGCTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTtg < 1:3260021/60‑1 (MQ=255) ccTGCTGGCGCGTAACCCGGCGCTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTTGTGTgcagca < 1:1955694/69‑1 (MQ=255) ctgGCGCGTAACCCGGCGCTGGAAGCGGCGGCCCt > 1:3635179/1‑35 (MQ=255) ctgGCGCGTAACCCGGCGCTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTTGTGTGCAGCAGACGc < 1:628907/70‑1 (MQ=255) gcgcGTAACCCGGCGTTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTTGTGTGCAGCAGACGCTg < 1:1456796/69‑1 (MQ=255) gcgcGTAACCCGGCGCTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTtg < 1:2422529/53‑1 (MQ=255) aaCCCGGCGCTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTTGTGTGCAGCAGACGCTGGAGCAggg > 1:4158760/1‑71 (MQ=255) cGGCGCTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTTGTGTGCAGCAGACGCTGGAGCAGGGtttt < 1:833593/71‑1 (MQ=255) | TTTCGTAACGTGCGTGCAGAAGATCTCTCCGCTCATTTAATGCGTAGCCTGCTGGCGCGTAACCCGGCGCTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTTGTGTGCAGCAGACGCTGGAGCAGGGTTTT > W3110S.gb/3607972‑3608106 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |