| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,633,778 | T→C | 14.1% | C97C (TGT→TGC) | yigG → | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,633,778 | 0 | T | C | 14.1% | 181.0 / 20.8 | 78 | C97C (TGT→TGC) | yigG | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (29/37); new base C (3/7); total (32/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.42e-01 | |||||||||||
CTTATGTTTTTGGTACATTAGCAGTATATATCATCTCTATCATCACAATGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAGTATGCTAATGCTTG > W3110S.gb/3633708‑3633842 | cTTATGTTTTTGGTACATTAGCAGTATATATCATCTCTATCATCACAATGATAGCCGAAGATTTTGCCTGt < 1:1213955/71‑1 (MQ=255) tGTTTTTGGTACATTAGCAGTATATATCATCTCTATCATCACAATGATAGCCGAAGATTTTGCCTGTTCa < 1:4289566/70‑1 (MQ=255) tttGGTACATTAGCAGTATATATCATCTCTATCATCACAATGATAGCCGAAGATTTTGCCTGAACAGGCa < 1:1479457/70‑1 (MQ=255) ttGGTACATTAGCAGTATATATCATCTCTATCATCACAATGATAGCCGAAGATTTTGCCTGAACAGGCa < 1:3387666/69‑1 (MQ=255) gTACATTAGCAGTATATATCATCTCTATCATCACAATGATAGCCGAAGATTTTGCCTGCTCAGGCAATgg < 1:1625362/70‑1 (MQ=255) ttAGCAGTATATATCATCTCTATCATCACAATGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCgat > 1:4319657/1‑71 (MQ=255) ttAGCAGTATATATCATCTCTATCATCACAATGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCgat > 1:26631/1‑71 (MQ=255) gTATATATCATCTCTATCATCACAATGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGAtttt < 1:4301344/71‑1 (MQ=255) tatataTCATCTCTATCATCACAATGATAGCCGAAGATTTTGCCTGtt < 1:2850330/48‑1 (MQ=255) tatataTCATCTCTATCATCACAATGATAGCCGAAGATTTTGCCTGtt < 1:963255/48‑1 (MQ=255) tatataTCATCTCTATCATCACAATGATAGCCGAAGATTTTGCCTGTTCAGGc < 1:3361942/53‑1 (MQ=255) tatatCATCTCTATCATCACAATGATAGCCGAAGATTTTGCCTGTTCAGGCaa > 1:1882327/1‑53 (MQ=255) tatatCATCTCTATCATCACAATGATAGCCGAAGATTTTGCCTGCTCAGGCaa > 1:2903749/1‑53 (MQ=255) tatCATCTCTATCATCACAATGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTg < 1:312639/70‑1 (MQ=255) tatCATCTCTATCATCACAATGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTg < 1:1942136/70‑1 (MQ=255) tatCATCTCTATCATCACAATGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGa > 1:1845766/1‑71 (MQ=255) tatCATCTCTATCATCACAATGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGa > 1:2129701/1‑71 (MQ=255) tcatcTCTATCATCACAATGATAGCCGAAGATTTTGCCTGTTc > 1:4074848/1‑43 (MQ=255) tcatcTCTATCATCACAATGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCgatgat > 1:2880750/1‑61 (MQ=255) atcTCTATCATCACAATGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGAttt < 1:2404385/70‑1 (MQ=255) atcTCTATCATCACAATGATAGCCGAAGATTTTGCCTGCTCAGGCAATGGTTCGATGATTTTTTTGATTTg < 1:4085395/71‑1 (MQ=255) tctcTATCATCACAATGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCg < 1:3057836/53‑1 (MQ=255) tctATCATCACAATGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGa < 1:1448568/64‑1 (MQ=255) tctATCATCACAATGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGAt < 1:995413/65‑1 (MQ=255) tctATCATCACAATGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTa > 1:3840859/1‑71 (MQ=255) tATCATCACAATGATAGCCGAAGATTTTGCCTGTTc < 1:1842592/36‑1 (MQ=255) tATCATCACAATGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTAtt > 1:2217980/1‑71 (MQ=255) tATCATCACAATGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTAtt > 1:1210945/1‑71 (MQ=255) atcatcACAATGATAGACGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGa > 1:2625874/1‑61 (MQ=255) tcatcaCAATGATAGCCGAAGATTTTGCCTGTTCAGGc < 1:2049267/38‑1 (MQ=255) tcatcaCAATGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTAtttt < 1:2380903/71‑1 (MQ=255) tcatcaCAATGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTAtttt < 1:376075/71‑1 (MQ=255) tcatcaCAATGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTAttt < 1:4304651/70‑1 (MQ=255) tcatcaCAATGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTAt < 1:3523883/68‑1 (MQ=255) catcaCAATGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTAttttt > 1:3497886/1‑71 (MQ=255) atcaCAATGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGtt > 1:1728318/1‑43 (MQ=255) atcaCAATGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTc > 1:783351/1‑71 (MQ=255) atcaCAATGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTc > 1:4340371/1‑71 (MQ=255) atcaCAATGATAGCCGAAGATTTTGCCTGCTCAGGCAATTGTTCGATGATTTTTTTGATTTGTTATTTTTc < 1:3543396/71‑1 (MQ=255) atcaCAATGATAGCCGAAGATTTTGCCTGCTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTc > 1:2694936/1‑71 (MQ=255) atcaCAATGATAGCCGAAGATTTTGCCTGCTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTc < 1:2988882/71‑1 (MQ=255) tcaCAATGATAGCCGAAGATTTTGCCTGTTCAGGCAATgg < 1:3573354/40‑1 (MQ=255) tcaCAATGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGt < 1:3900633/62‑1 (MQ=255) tcaCAATGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGt < 1:4438745/62‑1 (MQ=255) cacaATGATAGCCGAAGATTTTGCCTGTTCAGGCAATgg < 1:4173859/39‑1 (MQ=255) acaATGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCa < 1:2233636/70‑1 (MQ=255) caATGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTAttt < 1:522859/64‑1 (MQ=255) aaTGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACtt < 1:353620/71‑1 (MQ=255) aTGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACttt > 1:4278694/1‑71 (MQ=255) aTGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTT‑CACTTTc < 1:4369292/71‑1 (MQ=255) tGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACttt > 1:3013202/1‑70 (MQ=255) tGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTc < 1:2906631/71‑1 (MQ=255) tGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTc < 1:3887595/71‑1 (MQ=255) aTAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGAt < 1:2725145/50‑1 (MQ=255) aTAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCa < 1:3256140/70‑1 (MQ=255) aTAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAg < 1:4502805/71‑1 (MQ=255) aTAGCCGAAGATTTTGCCTGCTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCa < 1:2280202/70‑1 (MQ=255) tAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCgatgat > 1:1835110/1‑40 (MQ=255) aGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAGTa > 1:3695064/1‑71 (MQ=255) ccGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGAtttt < 1:1449156/40‑1 (MQ=255) cGAAGATTTTGCCTGCTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAGTa > 1:1531829/1‑68 (MQ=255) gAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAGTATGCt < 1:4333111/71‑1 (MQ=255) gAAGATTTTGCCTGCTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAGTATGCt < 1:163593/71‑1 (MQ=255) aaGATTTTGCCTGTTCAGGCAATGGTTCTATGATTTTTTTg < 1:2341314/41‑1 (MQ=255) aaGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTc < 1:1989430/55‑1 (MQ=255) gATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACt < 1:1991837/57‑1 (MQ=255) gATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAGTATGCTAAt > 1:4591750/1‑71 (MQ=255) gATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAGTATGCTAAt > 1:3977956/1‑71 (MQ=255) gATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAGTATGCTAAt > 1:2815151/1‑71 (MQ=255) aTTTTGCCTGCTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAGTATGCTAATg < 1:2502560/71‑1 (MQ=255) ttttGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAGTATGCTAATGc < 1:691209/71‑1 (MQ=255) tttGCCTGTTCAGGCAATGGTTCGATGAttttttt > 1:1054601/1‑35 (MQ=255) tttGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTa > 1:1086352/1‑44 (MQ=255) tttGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTa > 1:2264950/1‑44 (MQ=255) tttGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTa > 1:358994/1‑44 (MQ=255) ttGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCAc > 1:1501347/1‑52 (MQ=255) ttGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCAc > 1:731728/1‑52 (MQ=255) tGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAGTATGCTa > 1:2381479/1‑64 (MQ=255) gCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAGTATGCTAATGCTTg < 1:1238462/70‑1 (MQ=255) cTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCa < 1:2365014/53‑1 (MQ=255) gTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAGTATGCTAAt < 1:4177070/61‑1 (MQ=255) ttCAGGCAATGGTTCGATGATTTTTTTGATTTGTTAttttt > 1:4379221/1‑41 (MQ=255) ttCAGGCAATGGTTCGATGATTTTTTTGATTTGTTAttttt > 1:863393/1‑41 (MQ=255) ttCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAGTATGCt > 1:4316382/1‑57 (MQ=255) ttCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAGTATGCTa > 1:480356/1‑58 (MQ=255) | CTTATGTTTTTGGTACATTAGCAGTATATATCATCTCTATCATCACAATGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAGTATGCTAATGCTTG > W3110S.gb/3633708‑3633842 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |