| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,671,661 | G→A | 29.2% | R204Q (CGG→CAG) | rhlB → | ATP‑dependent RNA helicase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,671,661 | 0 | G | A | 29.2% | 79.6 / 40.4 | 55 | R204Q (CGG→CAG) | rhlB | ATP‑dependent RNA helicase |
| Reads supporting (aligned to +/- strand): ref base G (16/22); new base A (7/9); total (23/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.69e-01 | |||||||||||
TTCCGCCGTATGCCGCCTGCAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCGAATATATTGAAGTGGAACCGGA > W3110S.gb/3671597‑3671721 | ttCCGCCGTATGCCGCCTGCAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCg < 1:1121278/65‑1 (MQ=255) ccgccgTATGCCGCCTGCAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCAGGTACGt > 1:244755/1‑70 (MQ=255) cgccgTATGCCGCCTGCAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTg < 1:3030077/70‑1 (MQ=255) cgccgTATGCCGCCTGCAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTg < 1:2157654/70‑1 (MQ=255) gccgTATGCCGCCTGCAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCTGGTACGTg < 1:2311666/69‑1 (MQ=255) gccgTATGCCGCCTGCAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGaa < 1:4315532/71‑1 (MQ=255) ccgTATGCCGCCTGCAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCAGGTACGTGAAc < 1:1163992/71‑1 (MQ=255) tGCCGCCTGCAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCg > 1:3076658/1‑71 (MQ=255) tGCCGCCTGCAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCg > 1:2267598/1‑71 (MQ=255) tGCCGCCTGCAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCAGGTACGTGAACTGGCg > 1:2462812/1‑71 (MQ=255) cTGCAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGa < 1:2018832/58‑1 (MQ=255) tGCAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACg > 1:3310439/1‑54 (MQ=255) cAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAg > 1:4175115/1‑68 (MQ=255) cAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAg > 1:763092/1‑68 (MQ=255) cAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCa < 1:3025088/70‑1 (MQ=255) cAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCa < 1:146860/70‑1 (MQ=255) cAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCAGGTACGTGAACTGGCGTTCGAGc < 1:1937902/69‑1 (MQ=255) cAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCAGGTACGTGAACTGGCGTTCGAGc < 1:224891/69‑1 (MQ=255) cAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCAGGTACGTGAACTGGCGTTCGAGc < 1:1294114/69‑1 (MQ=255) aaaCCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGaa > 1:2204182/1‑55 (MQ=255) aaaCCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCa < 1:4610903/69‑1 (MQ=255) aaaCCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGa < 1:4313378/71‑1 (MQ=255) aaaCCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGa < 1:3482841/71‑1 (MQ=255) aaCCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAAc > 1:4620052/1‑55 (MQ=255) aaCCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGAt > 1:2976961/1‑71 (MQ=255) cAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGaa < 1:1994348/71‑1 (MQ=255) aGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGAAc < 1:4190319/71‑1 (MQ=255) gcgcCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCa < 1:3035332/63‑1 (MQ=255) gcCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCAGGTACGTGAACTGGCGTTCGAGCAGATGAAc < 1:3193312/68‑1 (MQ=255) cTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCg < 1:3303692/55‑1 (MQ=255) cTCAACATGCTGTTCTCCGCCACGCTTTCGTACCAGGTACGTGAACTGGCGTTCGAGCAGATGAACaa > 1:945581/1‑68 (MQ=255) tCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGAAc < 1:2611231/65‑1 (MQ=255) tCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGAACaa > 1:4141877/1‑67 (MQ=255) aaCATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCg < 1:861380/70‑1 (MQ=255) aaCATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCg > 1:1818504/1‑70 (MQ=255) aaCATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCg > 1:4443547/1‑70 (MQ=255) aaCATGCTGTTCTCCGCCACGCTTTCGTACCAGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCg > 1:1483438/1‑70 (MQ=255) aaCATGCTGTTCTCCGCCACGCTTTCGTACCAGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCg > 1:1211538/1‑70 (MQ=255) aCATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCGaa < 1:1959441/71‑1 (MQ=255) aCATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCGaa > 1:4568385/1‑71 (MQ=255) aTGCTGTTCTCCGCCACGCTTTCGTACCAGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCGAata > 1:2883804/1‑71 (MQ=255) tGCTGTTCTCCGCCACGCTTTCGTACCAGGTACGTg < 1:3115038/36‑1 (MQ=255) gCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCGAatat > 1:1618580/1‑70 (MQ=255) cTGTTCTCCGCCACTCATTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCg < 1:4593028/64‑1 (MQ=255) ttCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCGAATATATTg > 1:2826228/1‑70 (MQ=255) gctcCGCCACGCTTTCGTACCAGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCGAATATATTGaa < 1:2837735/70‑1 (MQ=255) ctcCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGAt < 1:471907/48‑1 (MQ=255) cGCCACGCTTTCGTACCAGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCGAATATATTGAAGTg > 1:4102145/1‑70 (MQ=255) ccACGCTTTCGTACCGGGTACGTGAACTGGCGTTCg < 1:1989309/36‑1 (MQ=255) ccACGCTTTCGTACCAGGTACGTGAACTGGCGTTCGAGCAGATGa < 1:3844257/45‑1 (MQ=255) gCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCGAATATATTGAAGTGGAACCgg < 1:1879369/71‑1 (MQ=255) cTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGAACaa > 1:183189/1‑44 (MQ=255) cTTTCGTACCAGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCGAATATATTGAAGTGGAACCGGa < 1:4173578/71‑1 (MQ=255) ttCGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCGAATATATTGa < 1:240348/57‑1 (MQ=255) cGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGAACaa > 1:4627422/1‑40 (MQ=255) tACCGGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCGaa < 1:253841/45‑1 (MQ=255) | TTCCGCCGTATGCCGCCTGCAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCGAATATATTGAAGTGGAACCGGA > W3110S.gb/3671597‑3671721 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |