| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 215,801 | A→G | 16.0% | E178G (GAG→GGG) | nlpE → | lipoprotein involved with copper homeostasis and adhesion |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 215,801 | 0 | A | G | 16.0% | 117.3 / 16.3 | 50 | E178G (GAG→GGG) | nlpE | lipoprotein involved with copper homeostasis and adhesion |
| Reads supporting (aligned to +/- strand): ref base A (23/19); new base G (2/6); total (25/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.88e-01 | |||||||||||
ATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGA > W3110S.gb/215741‑215864 | aTGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGGGCTGGAGc < 1:174519/69‑1 (MQ=255)aTGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGt < 1:2231957/71‑1 (MQ=255) tGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCg > 1:3168733/1‑69 (MQ=255) tGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCg > 1:3785159/1‑69 (MQ=255) tGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCg > 1:4266440/1‑69 (MQ=255) gcggcgACATTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGGGCTGGAGCGTAg > 1:2448796/1‑71 (MQ=255) ggcgACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCt < 1:709965/71‑1 (MQ=255) ggcgACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCt < 1:980507/71‑1 (MQ=255) gcgACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTa > 1:4365212/1‑71 (MQ=255) gcgACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTa > 1:2685187/1‑71 (MQ=255) cgACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGTAGCGTAGCTAc < 1:4273491/71‑1 (MQ=255) gACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGGGCTGGAGCGTAGCTAcc < 1:4079697/71‑1 (MQ=255) ccTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCt > 1:4638515/1‑70 (MQ=255) cTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTgg > 1:4449624/1‑71 (MQ=255) tCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTgg > 1:3473543/1‑69 (MQ=255) tCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCt > 1:588270/1‑71 (MQ=255) tCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCt > 1:2854530/1‑71 (MQ=255) cACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTa > 1:1852018/1‑63 (MQ=255) tGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTAc > 1:1389753/1‑61 (MQ=255) aTTGCGCGACCGGAAAAGGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTAc > 1:2837376/1‑59 (MQ=255) aTTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGGGCTGGAGCGTAGCTACCTGGCTgcgcg > 1:3930923/1‑70 (MQ=255) aTTGCGCGACCGGAAAACGTTTCATGGTAGCCAATAACGCAGAGCTg > 1:2625813/1‑47 (MQ=255) tGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCgg > 1:3577392/1‑71 (MQ=255) cgcgACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGt < 1:2225605/70‑1 (MQ=255) gcgACCGGAACACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTca < 1:138795/71‑1 (MQ=255) gACCTGAAAACGTTTCATGGTAGCGAATAACGCAGGGCTg < 1:1965259/40‑1 (MQ=38) ccGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAg > 1:489659/1‑46 (MQ=255) aaaaCGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGaaa < 1:3077537/71‑1 (MQ=255) aaaaCGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGaaa < 1:3969235/71‑1 (MQ=255) aaaCGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTAc > 1:1955634/1‑45 (MQ=255) aaaCGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTAc > 1:3859598/1‑45 (MQ=255) aaaCGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGaaaa < 1:2114407/71‑1 (MQ=255) aaaCGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGaaa > 1:1239817/1‑70 (MQ=255) aaCGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCt < 1:327090/42‑1 (MQ=255) aCGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAAc < 1:4206069/71‑1 (MQ=255) aCGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAAc > 1:2128004/1‑71 (MQ=255) cGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGc > 1:4431667/1‑39 (MQ=255) cGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAAcc < 1:2090114/71‑1 (MQ=255) tttCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCgg < 1:2195375/71‑1 (MQ=255) aTGGTAGCGAATAACGCAGGGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGtt < 1:3510695/71‑1 (MQ=255) aTGGTAGCGAATAACGCAGGGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGtt < 1:658292/71‑1 (MQ=255) aTGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGtt < 1:2824787/71‑1 (MQ=255) tGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTa < 1:2503295/71‑1 (MQ=255) gTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACt < 1:2467452/71‑1 (MQ=255) tAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTg > 1:3029579/1‑71 (MQ=255) cGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCa < 1:278055/71‑1 (MQ=255) tAACGCAGGGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAg < 1:674006/71‑1 (MQ=255) tAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTca < 1:3434423/42‑1 (MQ=255) aCGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAg > 1:267575/1‑69 (MQ=255) aCGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGa < 1:2811496/70‑1 (MQ=255) | ATGCGGCGACCTTCACTGATTGCGCGACCGGAAAACGTTTCATGGTAGCGAATAACGCAGAGCTGGAGCGTAGCTACCTGGCTGCGCGCGGTCACAGTGAAAAACCGGTGTTACTGTCAGTAGA > W3110S.gb/215741‑215864 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |