| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,678,974 | C→A | 17.8% | R38R (CGC→CGA) | ilvY → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,678,974 | 0 | C | A | 17.8% | 77.5 / 4.8 | 28 | R38R (CGC→CGA) | ilvY | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base C (11/12); new base A (2/3); total (13/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
CATTTTGGCCGCAGCGCGCGGGCGATGCACGTTAGCCCATCCACGCTCTCACGGCAGATTCAGCGCCTGGAAGAAGATCTCGGTCAGCCGCTGTTTGTGCGCGATAACCGCACGGTGACGCTGACTGAAGCGGGCG > W3110S.gb/3678909‑3679044 | cATTTTGGCCGCAGCGAGCGGGCGATGCACGTTAGCCCATCCACGCTCTCACGGCAGATTCAGCGCCTgg > 1:2345973/1‑70 (MQ=255) aGCGCGCGGGCGATGCACGTTAGCCCATCCACGCTCTCACGGCAGATTCAGCGCCTGGAAGAAGATCTCgg < 1:4331501/71‑1 (MQ=255) gATGCACGTTAGCCCATCCACGCTCTCACGGCAGATTCAGCGCCTGGAAGAAGATCTCGGTCAGCCGCTGt < 1:1270794/71‑1 (MQ=255) tggaCGTTAGCCCATCCACGCTCTCACGGCAGATTCAGCGCCTGGAAGAAGATCTCGGTCAGCCGc < 1:1847670/63‑1 (MQ=255) tGCACGTTAGCCCATCCACGCTCTCACGGCAGATTCAGCGCCTGGAAGAAGATCTCGGTCAGCCGCTGttt > 1:614499/1‑71 (MQ=255) cGTTAGCCCATCCACGCTCTCACGGCAGATTCAGCGACTgg < 1:3055685/41‑1 (MQ=255) ttAGCCCATCCACGCTCTCACGGCAGATTCAGCGACTGGAAGAAGATCTCGGTCAGCCGCTGTTTGTgcgc > 1:1260772/1‑71 (MQ=255) cccATCCACGCTCTCACGGCAGATTCAGCGCCTGGAAGAAGATCTCGGTCAGCCGCTGTTTGTGCGCGAt < 1:2833974/70‑1 (MQ=255) cATCCACGCTCTCACGGCAGATTCAGCGCCTGGAAGAAGATCTCGGTCAGCCGCTGTTTGTgcgc < 1:41940/65‑1 (MQ=255) aTCCACGCTCTCACGGCAGATTCAGCGCCTGGAAGAAGATCTCGGTCAg < 1:218662/49‑1 (MQ=255) tCCACGCTCTCACGGCAGATTCAGCGCCTGGAAGAAGAt < 1:2889497/39‑1 (MQ=255) tCCACGCTCTCACGGCAGATTCAGCGCCTGGAAGAAGATCTCGGTCAGCCGCt < 1:585217/53‑1 (MQ=255) cACGCTCTCACGGCAGATTCAGCGCCTGGAAGAAGAt > 1:1937372/1‑37 (MQ=255) cACGCTCTCACGGCAGATTCAGCGCCTGGAAGAAGATCTCGGTCAGCCGCTGTTtgtg > 1:1085217/1‑58 (MQ=255) cACGCTCTCACGGCAGATTCAGCGCCTGGAAGAAGATCTCGGTCAGCCGCTGTTTGTGCGCGATAACCGCa > 1:1145701/1‑71 (MQ=255) cACGCTCTCACGGCAGATTCAGCGCCTGGAAGAAGATCTCGGTCAGCCGCTGTTTGTGCGCGATAACCGCa > 1:3624607/1‑71 (MQ=255) cACGCTCTCACGGCAGATTCAGCGCCTGGAAGAAGATCTCGGTCAGCCGCTGTTTGTGCGCGATAACCGCa > 1:1913951/1‑71 (MQ=255) cACGCTCTCACGGCAGATTCAGCGACTGGAAGAAGATCTCGGTCAGCCGCTGTTTGTGCGCGATAACCGc < 1:4202721/70‑1 (MQ=255) cACGCTCTCACGGCAGATTCAGCGACTGGAAGAAGATCTCGGTCAGCCGCTGTTTGTGCGCGATAACCGCa > 1:3216770/1‑71 (MQ=255) aCGCTCTCACGGCAGATTCAGCGCCTGGAAGAAGATCTCGGTCAGCCGCTGTTTGTGCGCGATAACCGCAc < 1:230704/71‑1 (MQ=255) ctctCACGGCAGATTCAGCGCCTGGAAGAAGATCTCGGTCAGCCGCTGTTTGTGCGCGATAACCGCACGGt < 1:846818/71‑1 (MQ=255) ctctCACGGCAGATTCAGCGCCTGGAAGAAGATCTCGGTCAGCCGCTGTTTGTGCGCGATAACCGCACGGt < 1:4437508/71‑1 (MQ=255) tctcACGGCAGATTCAGCGCCTGGAAGAAGATCTCGGTCAGCCGCTGTTTGTGCGCGATAACCGCACGGt < 1:1396759/70‑1 (MQ=255) ctcACGGCAGATTCAGCGACTGGAAGAAGATCTCGGTCAGCCGCTGTTTGTGCGCGATAACCGCACGGt < 1:2949550/69‑1 (MQ=255) aGATTCAGCGCCTGGAAGAAGATCTCGGTCAGCCGCTGTTTGTGCGCGATAACCGCACGGTGACGctgact > 1:3684178/1‑71 (MQ=255) tCAGCGCCTGGAAGAAGATCTCGGTCAGCCGCTGTTTGTGCGCGATAACCGCACGGTGa > 1:1488669/1‑59 (MQ=255) tCAGCGCCTGGAAGAAGATCTCGGTCAGCCGCTGTTTGTGAGCGATAACCGCACGGTGACGCTGACTGAAg > 1:3096985/1‑71 (MQ=255) cAGCGCCTGGAAGAAGATCTCGGTCAGCCGCTGTTTGTGCGCGATAACCGCACGGTGACGCTGACTGAAGc > 1:766752/1‑71 (MQ=255) gcgcCTGGAAGAAGATCTCGGTCAGCCGCTGTTTGTGCGCGATa < 1:4011548/44‑1 (MQ=255) cgcCTGGAAGAAGATCTCGGTCAGCCGCTGTTTGTGCGCGATAACCGCACGGTGACGCTGACTGAAGCggg > 1:3920268/1‑71 (MQ=255) gcCTGGAAGAAGATCTCGGTCAGCCGCTGTTTGTGCGCGATa < 1:82027/42‑1 (MQ=255) gcCTGGAAGAAGATCTCGGTCAGCCGCTGTTTGTGCGCGATa < 1:87430/42‑1 (MQ=255) ccTGGAAGAAGATCTCGGTCAGCCGCTGTTTGTGCGCGATAACCGCACGGTGACGCTGACTGAAGCGGGCg < 1:3616308/71‑1 (MQ=255) | CATTTTGGCCGCAGCGCGCGGGCGATGCACGTTAGCCCATCCACGCTCTCACGGCAGATTCAGCGCCTGGAAGAAGATCTCGGTCAGCCGCTGTTTGTGCGCGATAACCGCACGGTGACGCTGACTGAAGCGGGCG > W3110S.gb/3678909‑3679044 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |