| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,699,899 | G→A | 21.2% | G169G (GGC→GGT) | rbsB ← | D‑ribose transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,699,899 | 0 | G | A | 21.2% | 67.7 / 13.4 | 33 | G169G (GGC→GGT) | rbsB | D‑ribose transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (12/14); new base A (4/3); total (16/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01 | |||||||||||
ATCAAAATCTGCTGGCTGGCTGGCAAGAACATTAAACTTGTGAGCAGCAACGGCCTGCTGGAAGCCTTCGCCACGTTCACGGGCTGCGGATGTACCAGCAATGCCTTGCAGCTCGATAACTTTGGCACCTTC > W3110S.gb/3699836‑3699967 | aTCAAAATCTGCTGGCTGGCTGGCAAGAACATTAAACTTGTGAGCAGCAACGGCCTGCTGGAAGCCTTCGc < 1:2432585/71‑1 (MQ=255) cAAAATCTGCTGGCTGGCTGGCAAGAACATTAAACTTGTGAGCAGCAACGGCCTGCTGGAAGCCTTCGCCa > 1:4371220/1‑71 (MQ=255) aaaaTCTGCTGGCTGGCTGGCAAGAACATTAAACTTGTGAGCAGCAACGGCCTGCTGGAAACCTTCGCCAc > 1:4022648/1‑71 (MQ=255) aTCTGCTGGCTGGCTGGCAAGAACATTAAACTTGTGAGCAGCAACGGCCTGCTGGAAGCCTTCTCCACGtt < 1:153322/71‑1 (MQ=255) tgctgGCTGGCTGGCAAGAACATTAAACTTGTGAGCAGCAACGGCCTGCTGGAAACCTTCGCCACGTTCAc > 1:1857847/1‑71 (MQ=255) ctggctggcAAGAACATTAAACTTGTGAGCAGCAACGGCCTGCTGGAAGCCTTCGcc < 1:4152719/57‑1 (MQ=255) ctggcAAGAACATTAAACTTGTGAGCAGCAACGGCCTGCTGGAAGCCTTCGc > 1:4179914/1‑52 (MQ=255) ctggcAAGAACATTAAACTTGTGAGCAGCAACGGCCTGCTGGAAGCCTTCGCCACGTTCACGGGCTGCGGa < 1:1410235/71‑1 (MQ=255) aaGAACATTAAACTTGTGAGCAGCAACGGCCTGCTGGAAGCCTTCGcc < 1:4303357/48‑1 (MQ=255) aCATTAAACTTGTGAGCAGCAACGGCCTGCTGGAAGCCTTCGCCACGTTCACGGGCTGCGGATGTACCAGc < 1:2032472/71‑1 (MQ=255) aCATTAAACTTGTGAGCAGCAACGGCCTGCTGGAAACCTTCGCCACGTTCACGGGCTGCGGATGTACCAGc < 1:348206/71‑1 (MQ=255) aCATTAAACTTGTGAGCAGCAACGGCCTGCTGGAAACCTTCGCCACGTTCACGGGCTGCGGATGTACCAGc < 1:2272000/71‑1 (MQ=255) aCATTAAAATTGTGAGCAGCAACGGCCTGCTGGAAGCCTTCGCCACGTTCACggg > 1:1543795/1‑55 (MQ=255) ttAAACTTGTGAGCAGCAACGGCCTGCTGGAAACCTTCGCCACGTTCACGGGCTGCGGATGTACCAGCAAt > 1:4243681/1‑71 (MQ=255) tAAACTTGTGAGCAGCAACGGCCTGCTGGAAGCCTTCGCCACGTTCACGGGCTGCGGATGTACCAGCAATg > 1:814052/1‑71 (MQ=255) aaaCTTGTGAGCAGCAACGGCCTGCTGGAAGCCTTCGCCACGTTCa > 1:3239925/1‑46 (MQ=255) aaaCTTGTGAGCAGCAACGGCCTGCTGGAAACCTTCg > 1:3934400/1‑37 (MQ=255) aaaCTTGTGAGCAGCAACGGCCTGCTGGAAACCTTCGCCACGTTCACGGGCTGCGGATGTACCAGCAATg < 1:787786/70‑1 (MQ=255) cTTGTGAGCAGCAACGGCCTGCTGGAAGCCTTCGCCACGTTCACGGGCt > 1:2021016/1‑49 (MQ=255) ttGTGAGCAGCAACGGCCTGCTGGAAGCCTTCGCCACGTTCACg < 1:3296323/44‑1 (MQ=255) gtgAGCAGCAACGGCCTGCTGGAAGCCTTCGCCACGTTCACGGGCTGCGGATGTACCAGCaa > 1:4472600/1‑62 (MQ=255) gtgAGCAGCAACGGCCTGCTGGAAGCCTTCGCCACGTTCACGGGCTGCGGATGTACCAGCAATGCCTTg < 1:3599948/69‑1 (MQ=255) gtgAGCAGCAACGGCCTGCTGGAAGCCTTCGCCACGTTCACGGGCTGCGGATGTACCAGCAATGCCTTg < 1:3648915/69‑1 (MQ=255) agcagcAACGGACTGCTGGAAGCCTTCGCCACGTTCACGGGCTGCGGATGTACCAGCAATGCCTTGCAGc > 1:1568897/1‑70 (MQ=255) gcagcaACGGCCTGCTGGAAGCCTTCGCCACGTTCACGGGCTGCGGATGTACCAGCaa > 1:4073290/1‑58 (MQ=255) cagcaACGGCCTGCTGGAAGCCTTCGCCACGTTCACGGGCTGCGGATGTACCAGCAATGCCTTGCAGCTc > 1:3297107/1‑70 (MQ=255) caACGGCCTGCTGGAAGCCTTCGCCACGTTCACGGGCTGCGGATGTACCAGCAATGCCTTGCAGCTCGAt < 1:3463977/70‑1 (MQ=255) gCCTGCTGGAAGCCTTCGCCACGTTCACGGGCTGCGGATGTACCAGCAATGCCTTGCAGCTCGATAACttt < 1:2809634/71‑1 (MQ=255) ctgctgGAAGCCTTCGCCACGTTCACGGGCTGCGGATGTACCAGCAATGCCTTGCAGCTCGATAACTTTgg < 1:1661860/71‑1 (MQ=255) ctgGAAGCCTTCGCCACGTTCACGGGCTGCGGATGTAc > 1:1293106/1‑38 (MQ=255) ggAAGCCTTCGCCACGTTCACGGGCTGCGGATGTACCAGCAATGCCTTGCAGCTCGATAACTTTg > 1:1809888/1‑65 (MQ=255) gAAGCCTTCGCCACGTTCACGGGCTGCGGATGTACCAGCAATGCCTTGCAGCTCGATAACTTTGGCAcc < 1:2569300/69‑1 (MQ=255) aaGCCTTCGCCACGTTCACGGGCTGCGGATGTACCAGCAATGCCTTGCAGCTCGATAACTTTGGCACCTTc < 1:2768274/71‑1 (MQ=255) gCCTTCGCCACGTTCACGGGCTGCGGATGTACCAGCAATGCCTTGCAGCTCGATAACTTTGGCACCtt < 1:1071715/68‑1 (MQ=255) | ATCAAAATCTGCTGGCTGGCTGGCAAGAACATTAAACTTGTGAGCAGCAACGGCCTGCTGGAAGCCTTCGCCACGTTCACGGGCTGCGGATGTACCAGCAATGCCTTGCAGCTCGATAACTTTGGCACCTTC > W3110S.gb/3699836‑3699967 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |