| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,701,075 | T→C | 30.4% | L107L (TTA→TTG) | rbsC ← | D‑ribose transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,701,075 | 0 | T | C | 30.4% | 58.9 / 35.9 | 46 | L107L (TTA→TTG) | rbsC | D‑ribose transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (10/22); new base C (10/4); total (20/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.21e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01 | |||||||||||
GCGATAAACGCCTGGACGCGACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATGCCGACGATAGATGCAGCAACTGCGC > W3110S.gb/3701010‑3701145 | gCGATAAACGCCTGGACGCGACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCCAAcgc > 1:2248864/1‑71 (MQ=255)gCGATAAACGCCTGGACGCGACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCCAAcgc > 1:1945899/1‑71 (MQ=255) cGATAAACGCCTGGGCGCGACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCTAAcgc > 1:4327877/1‑70 (MQ=255) aaaCGCCTGGACGCGACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCTAACGCGagag > 1:1886819/1‑71 (MQ=255) aaaCGCCTGGACGCGACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCCAACGCga > 1:895918/1‑68 (MQ=255) aaaCGCCTGGACGCGACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCCAACGCGagag > 1:2853903/1‑71 (MQ=255) cGCCTGGACGCGACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCTAACGCGAGagcag < 1:307502/71‑1 (MQ=255) cGCCTGGACGCGACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCTAACGCGAGagcag < 1:1761896/71‑1 (MQ=255) gACGCGACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCTAACGCGAGagcagcagc < 1:2438725/69‑1 (MQ=255) gACGCGACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCg > 1:3259539/1‑70 (MQ=255) gACGCGACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGa > 1:2626271/1‑71 (MQ=255) gACGCGACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGa < 1:1355139/71‑1 (MQ=255) gACGCGACCTTTCGCTACAATCACCCCGGTAACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGa > 1:1845479/1‑71 (MQ=255) gACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGACAGc > 1:4500258/1‑70 (MQ=255) cTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCTCCCAACGCGAGAGCAGCAGCGACAGccacc < 1:336625/71‑1 (MQ=255) cTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCTa < 1:2763347/45‑1 (MQ=255) tttCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCCAACGCGAGAGCAGCAGCGa > 1:3051819/1‑62 (MQ=255) ttCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCTAACGCGAGagcagcag > 1:3383618/1‑58 (MQ=255) tACAATCACCCCGGTTACCGCACCAATTGCGGCACCTAACGCga > 1:3593241/1‑44 (MQ=255) cAATCACCCCGGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCAtt < 1:2854155/70‑1 (MQ=255) cAATCACCCCGGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTg < 1:835413/71‑1 (MQ=255) cAATCACCCCGGTTACCGCACCAATTGCGGCACCCAACGCGAGAGCAGCAGCGACAGCCACCAGCGCAtt < 1:4419102/70‑1 (MQ=255) aTCACCCCGGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGAc < 1:4446267/71‑1 (MQ=255) tCACCCCGGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACt < 1:2948581/71‑1 (MQ=255) tCACCCCGGTTACCGCACCAATTGCGGCACCCAACGCGAGAGCAGCAGCGCCAGCCACCAGCGCATTGa > 1:1295865/1‑69 (MQ=255) aCCCCGGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTc < 1:2170428/71‑1 (MQ=255) aCCCCGGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTc < 1:3720405/71‑1 (MQ=255) aCCCCGGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTc < 1:3221744/71‑1 (MQ=255) aCCCCGGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTc < 1:1606238/71‑1 (MQ=255) cGGTTACCGCACCAATTGCGGCACCCAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTc < 1:883910/67‑1 (MQ=255) ttACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTc < 1:758628/64‑1 (MQ=255) aCCGCACCAATTGCGGCACCCAACGCGAGagcagca > 1:2588609/1‑36 (MQ=255) aCCGCACCAATTGCGGCACCCAACGCGAGAGCAGCAGCGACAGc < 1:2749176/44‑1 (MQ=255) aaTTGCGGCACCTAACGCGAGAGCAGCAGCGACAGccacc > 1:4269707/1‑40 (MQ=255) aaTTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACt < 1:580920/52‑1 (MQ=255) ttGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACt < 1:201101/50‑1 (MQ=255) ttGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATGCCGACGATAGATgc < 1:3018083/70‑1 (MQ=255) tGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATGCCGACGATAGATgcag > 1:2580193/1‑71 (MQ=255) gCGGCACCCAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATGCCGACGATAGATgcag > 1:3859240/1‑70 (MQ=255) gCGGCACCCAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATGCCGACGATAGATgcag > 1:2582747/1‑70 (MQ=255) cACCCAACGCGAGAGCAGAAGCGACAGCCACCAGCGCATTGACTTCaa > 1:887317/1‑48 (MQ=38) ccTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATGCCGACGATAGATgcagca < 1:109284/66‑1 (MQ=255) ccTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATGCCGACGATAGATGCAGCAACTgc < 1:1893667/71‑1 (MQ=255) cTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTc < 1:4375767/43‑1 (MQ=255) cTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTc < 1:3600293/43‑1 (MQ=255) cTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTc < 1:1906354/43‑1 (MQ=255) tAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCaa < 1:3981423/44‑1 (MQ=255) tAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATGCCGACGATAGATGCAGCAACTgcgc > 1:1034713/1‑71 (MQ=255) tAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATGACGACGATAGa > 1:3631604/1‑57 (MQ=255) | GCGATAAACGCCTGGACGCGACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATGCCGACGATAGATGCAGCAACTGCGC > W3110S.gb/3701010‑3701145 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |