| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,712,033 | G→A | 25.4% | M328I (ATG→ATA) | gidA → | glucose‑inhibited cell‑division protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,712,033 | 0 | G | A | 25.4% | 97.3 / 33.6 | 55 | M328I (ATG→ATA) | gidA | glucose‑inhibited cell‑division protein |
| Reads supporting (aligned to +/- strand): ref base G (25/16); new base A (9/5); total (34/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.40e-01 | |||||||||||
TTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGATCCT > W3110S.gb/3711969‑3712099 | tttATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAggg > 1:1839438/1‑70 (MQ=255) cGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATACAGGGGATGGaa < 1:3152231/71‑1 (MQ=255) tcCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGaaa > 1:1855730/1‑61 (MQ=255) caccaGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAg < 1:321355/67‑1 (MQ=255) caccaGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATACAGGGGATGGAAAACGCGAAGATc < 1:3892395/70‑1 (MQ=255) ccaGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATACAGGGGATGGAAAACGCGAAGATCGTg > 1:3263205/1‑71 (MQ=255) ccTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGAt < 1:3067756/63‑1 (MQ=255) tGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCg > 1:766080/1‑63 (MQ=255) gCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCgg > 1:2749309/1‑71 (MQ=255) ccGTTCGATGTGCAGATGCAAATCGTCCGCTCTATACAGGGGATGGAAAACGCGAAGATCGTGCGTCCggg > 1:3234494/1‑71 (MQ=255) cGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGt < 1:4345367/71‑1 (MQ=255) cGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGt < 1:944343/71‑1 (MQ=255) cGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCCTCCGGGt < 1:3639237/71‑1 (MQ=255) gTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGt < 1:252274/70‑1 (MQ=255) ttCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGAt < 1:251853/56‑1 (MQ=255) tCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTAt > 1:2994045/1‑71 (MQ=255) cGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTAt > 1:2382374/1‑70 (MQ=255) cGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATg > 1:3762103/1‑71 (MQ=255) cGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATg > 1:1967957/1‑71 (MQ=255) cGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATg > 1:1836558/1‑71 (MQ=255) cGATGTGCAGATGCAAATCGTCCGCTCTATACAGGGGATGGaaa > 1:4411781/1‑44 (MQ=255) cGATGTGCAGATGCAAATCGTCCGCTCTATACAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTAt > 1:3418452/1‑70 (MQ=255) gATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGc < 1:3818358/71‑1 (MQ=255) gATGTGCAGATGCAAATCGTCCGCTCTATACAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGc < 1:1723193/71‑1 (MQ=255) tgtgCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCa > 1:1322646/1‑71 (MQ=255) gtgCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGt > 1:854745/1‑63 (MQ=255) tgCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAg < 1:1939775/47‑1 (MQ=255) tgCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAg < 1:888135/47‑1 (MQ=255) gCAGATGCAAATCGTCCGCTCTATACAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCAtt > 1:2526546/1‑70 (MQ=255) cAGATGCAAATCGTCCGCTCTATGCAGGGGATGGaa > 1:2119089/1‑36 (MQ=255) cAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTg < 1:4155191/70‑1 (MQ=255) gATGCAAATCGTCCGCTCTATACAGGGGATGGAAAAcgcg < 1:3264128/40‑1 (MQ=255) gATGCAAATCGTCCGCTCTATACAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCAtt > 1:135375/1‑67 (MQ=255) tGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGtt < 1:993885/57‑1 (MQ=255) tGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGtt < 1:3050902/57‑1 (MQ=255) tGCAAATCGTCCGCTCTATACAGGGGATGGAAAACGCGaa > 1:4166329/1‑40 (MQ=255) gCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTa > 1:10809/1‑57 (MQ=255) gCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTa > 1:2232132/1‑57 (MQ=255) gCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTa > 1:1352121/1‑57 (MQ=255) gCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTa > 1:4367743/1‑57 (MQ=255) gCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTAt > 1:132193/1‑70 (MQ=255) gCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTAt > 1:40783/1‑70 (MQ=255) aaTCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATc > 1:1183828/1‑40 (MQ=255) aaTCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATc > 1:4008406/1‑40 (MQ=255) aTCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGActt > 1:3370594/1‑71 (MQ=255) aTCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGAct > 1:539140/1‑70 (MQ=255) gTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTc > 1:3286048/1‑43 (MQ=255) gTCCGCTCTATACAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGActtctt < 1:2112133/71‑1 (MQ=255) cGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCg < 1:1503641/70‑1 (MQ=255) cGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGa < 1:4278741/71‑1 (MQ=255) cGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGa > 1:3039460/1‑71 (MQ=255) ctATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGATCCt > 1:2486137/1‑71 (MQ=255) ctATACAGGGGATGGAAAACGCGAAGATCGTGCGTc > 1:3836552/1‑36 (MQ=255) ctATACAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGa > 1:1782797/1‑58 (MQ=255) tATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGATcc < 1:56581/69‑1 (MQ=255) | TTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGATCCT > W3110S.gb/3711969‑3712099 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |