| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,730,340 | C→T | 12.9% | T75T (ACC→ACT) | bglG → | transcriptional antiterminator of the bgl operon |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,730,340 | 0 | C | T | 12.9% | 169.8 / 14.1 | 62 | T75T (ACC→ACT) | bglG | transcriptional antiterminator of the bgl operon |
| Reads supporting (aligned to +/- strand): ref base C (21/33); new base T (6/2); total (27/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.89e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01 | |||||||||||
GAGCAGTCATGAACTGAACGGGCGATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGAAAATTACAGGACAGTATTTATATCTCGC > W3110S.gb/3730271‑3730407 | gAGCAGTCATGAACTGAACGGGCGATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCt > 1:819869/1‑71 (MQ=255) aTGAACTGAACGGGCGATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACTTGTGATCg > 1:3245951/1‑70 (MQ=255) aTGAACTGAACGGGCGATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCg > 1:606752/1‑70 (MQ=255) aTGAACTGAACGGGCGATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCg > 1:4573120/1‑70 (MQ=255) gaactgaacGGGCGATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGtat > 1:912507/1‑71 (MQ=255) gaactgaacGGGCGATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGtat > 1:587250/1‑71 (MQ=255) aactgaacGGGCGATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGtatt < 1:30448/71‑1 (MQ=255) aacGGGCGATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGtatta > 1:126559/1‑67 (MQ=255) aacGGGCGATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTAtct > 1:1649558/1‑70 (MQ=255) aacGGGCGATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTAtct > 1:1689451/1‑70 (MQ=255) aacGGGCGATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTAtct > 1:1955049/1‑70 (MQ=255) ggCGATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACTTGTGATCGTATTATCTCTTTa < 1:2808628/71‑1 (MQ=255) gATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTTATGGCAACCTGTGATCGTATTATCTCttt < 1:3533774/67‑1 (MQ=255) gATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGta < 1:1718205/57‑1 (MQ=255) gATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGta < 1:1405156/57‑1 (MQ=255) gATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAgc < 1:2990398/70‑1 (MQ=255) aTTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGtatta < 1:2002674/59‑1 (MQ=255) ttAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACTTGTGATCGTATTATCTCTTTAgcg > 1:1789417/1‑69 (MQ=255) ttAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCg < 1:37837/53‑1 (MQ=255) ttAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCg < 1:2682076/53‑1 (MQ=255) tAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGta > 1:3030619/1‑54 (MQ=255) aGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAgg < 1:1160887/70‑1 (MQ=255) aGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGa < 1:4290961/71‑1 (MQ=255) cGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAgc < 1:1896972/71‑1 (MQ=255) gAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAgcg < 1:4504729/71‑1 (MQ=255) aaCTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAgcgc > 1:4635955/1‑71 (MQ=255) aCTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCt < 1:3089046/71‑1 (MQ=255) tctTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGa < 1:1770382/40‑1 (MQ=255) tctTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGt < 1:2767226/44‑1 (MQ=255) tctTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCa < 1:3536429/61‑1 (MQ=255) ttAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCg < 1:4429698/41‑1 (MQ=255) ttAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCg < 1:2023458/41‑1 (MQ=255) aGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAg > 1:324512/1‑57 (MQ=255) aGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAg > 1:2529611/1‑57 (MQ=255) gTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAgc < 1:4619278/60‑1 (MQ=255) tCATATTCCTCTTGAGGTGATGGCAACTTGTGATCGTATTAtc < 1:4486011/43‑1 (MQ=255) tCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGAAAAt < 1:447604/71‑1 (MQ=255) atatTCCTCTTGAGGTGATGTCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGAAAATTa > 1:1827317/1‑71 (MQ=255) atatTCCTCTTGAGGTGATGGCAACTTGTGATCGTATTAtctc > 1:1489145/1‑43 (MQ=255) atatTCCTCTTGAGGTGATGGCAACTTGTGATCGTATTATCTCTTTAGCGCa > 1:307050/1‑52 (MQ=255) atatTCCTCTTGAGGTGATGGCAACTTGTGATCGTATTATCTCTTTAGCGCa > 1:2477390/1‑52 (MQ=255) atatTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGAAAATTa < 1:284717/71‑1 (MQ=255) atatTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGAAAATTa > 1:3498366/1‑71 (MQ=255) atatTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGAAAATTa < 1:50590/71‑1 (MQ=255) atatTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGAAAATTa < 1:2605880/71‑1 (MQ=255) tatTCCTCTTGAGGTGATGGCAACCTGTGATCTTATTATCTCTTTAGCGCAGGAGCGCTTGGGaa < 1:295484/65‑1 (MQ=255) tatTCCTCTTGAGGTGATGGCAACCTGTGATCGta < 1:4307132/35‑1 (MQ=255) tatTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAgcgc < 1:4331156/50‑1 (MQ=255) tatTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAgcg < 1:620182/57‑1 (MQ=255) ttCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGAAAATTACAg < 1:381914/71‑1 (MQ=255) ccTCTTGAGGTGATGGCAACCTGTGATCGTATTAtc > 1:2609298/1‑36 (MQ=255) ctTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGAAAATTACAGGACAg > 1:2595446/1‑71 (MQ=255) gAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCATGAGCGCTTGGGAAAATTACAGGACAGTa < 1:1006190/70‑1 (MQ=255) gTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGAAAATTa < 1:4480762/57‑1 (MQ=255) tGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGAAAATTACAGGACAGTATTtat < 1:1231718/71‑1 (MQ=255) aTGGCAACCTGTGATCGTATTATCTCTTTAGCGCAgg > 1:1169803/1‑37 (MQ=255) aTGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGaa < 1:4047101/49‑1 (MQ=255) tGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGAAAATTACAGGACAGTAtt < 1:3410516/65‑1 (MQ=255) gCAACTTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTg > 1:3980560/1‑42 (MQ=255) gCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGAAAATTACAGGACAGTATTTATAtctc > 1:4483004/1‑71 (MQ=255) aaCCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGAAAATTACAGGACAGTATTTATATCTCGc > 1:878939/1‑71 (MQ=255) aaCCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGAAAATTACAGGACAGTATTTATATCTCGc > 1:2198386/1‑71 (MQ=255) | GAGCAGTCATGAACTGAACGGGCGATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGAAAATTACAGGACAGTATTTATATCTCGC > W3110S.gb/3730271‑3730407 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |