| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,740,943 | C→T | 31.2% | R290R (CGC→CGT) | yieG → | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,740,943 | 0 | C | T | 31.2% | 63.4 / 37.8 | 48 | R290R (CGC→CGT) | yieG | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (13/20); new base T (9/6); total (22/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.23e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.43e-01 | |||||||||||
CTCCGGTACGCTGATTGGCGTGACCGATAAAGCAGGTCTGGCGGATGAGAAGGGGAAATTCCCGCGCATGAAGCAGGCGCTGTATGTCGACAGTATCTCTTCCGTGACCGGTTCGTTTATCGGTACTTCTTCCGT > W3110S.gb/3740877‑3741011 | ctccGGTACGCTGATTGGCGTGACCGATAAAGCAGGTCTGGCGGATGAGAAGGGGAAATTCCCGCGCATg > 1:560640/1‑70 (MQ=255) cGGTACGCTGATTGGCGTGACCGATAAAGCAGGTCTGGCGGATGAGAAGGGGAAATTCCCGCGCATGAAg > 1:2630411/1‑70 (MQ=255) ggTACGCTGATTGGCGTGACCGATAAAGCAGGTCTGGCGGATGAGAAGGGGAAATTCCCGCGTATGAAGc < 1:2919432/70‑1 (MQ=255) tACGCTGATTGGCGTGACCGATAAAGCAGGTCTGGCGGATGAGAAGGGGAAATTCCCGCGTATGAAGCAgg > 1:3082165/1‑71 (MQ=255) aCGCTGATTGGCGTGACCGATAAAGCAGGTATGGCGGATGAGAAGGGGAAATTCCCGCGCATGAAGCAgg > 1:1585839/1‑70 (MQ=255) cGCTGATTGGCGTGACCGATAAAGCAGGTCTGGCGGATGAGAAGGGGAAATTCCCGCGTATGAAGCAGgc > 1:1926002/1‑70 (MQ=255) cGCTGATTGGCGTGACCGATAAAGCAGGTCTGGCGGATGAGAAGGGGAAATTCCCGCGCATGAAGCAGgc > 1:4015072/1‑70 (MQ=255) cTGATTGGCGTGACCGATAAAGCAGGTCTGGCGGATGAGAAGGGGAAATTCCCGCGTATGAAGCAGGCGCt > 1:1959862/1‑71 (MQ=255) gATTGGCGTGACCGATAAAGCAGGTCTGGCGGATGAGAAGGGGAAATTCCCGCGCATGAAGCAGGCCCt < 1:1985442/69‑1 (MQ=255) aTTGGCGTGACCGATAAAGCAGGTCTGGCGGATGAGAAGGGGAAATTCCCGCGCATGAAGCAGGCGCTg < 1:3503910/69‑1 (MQ=255) aTTGGCGTGACCGATAAAGCAGGTCTGGCGGATGAGAAGGGGAAATTCCCGCGCATGAAGCAGGCGCTg < 1:195541/69‑1 (MQ=255) ttGGCGTGACCGATAAAGCAGCTCTGGCGGATGAGAAGGGGAAATTCCCGCGTATGAAGCAGGCGCTGTAt < 1:4102256/71‑1 (MQ=255) ggCGTGACCGATAAAGCAGGTCTGGCGGATGAGAAGGGGAAATTCCCGCGTATGAAGCAGGCGCTGTa > 1:3574955/1‑68 (MQ=255) tGACCGATAAAGCAGGTCTGGCGGATGAGAAGGGGAAATTCCCGCGCATGAAGCAGGCGCTGTATGTCGa < 1:173954/70‑1 (MQ=255) tGACCGATAAAGCAGGTCTGGCGGATGAGAAGGGGAAATTCCCGCGCATGAAGCAGGCGCTGTATGTCGAc < 1:3025362/71‑1 (MQ=255) tGACCGATAAAGCAGGTCTGGCGGATGAGAAGGGGAAATTCCCGCGCATGAAGCAGGCGCTGTATGTCGAc < 1:3139486/71‑1 (MQ=255) aCCGATAAAGCAGGTCTGGCGGATGAGAAGGGGAAATTCCCGCGTAt > 1:2997193/1‑47 (MQ=255) aCCGATAAAGCAGGTCTGGCGGATGAGAAGGGGAAATTCCCGCGTATGAAGCAGGCGCTGTATGTCGACa < 1:2331126/70‑1 (MQ=255) aCCGATAAAGCAGGTCTGGCGGATGAGAAGGGGAAATTCCCGCGCATGaa < 1:1128346/50‑1 (MQ=255) aCCGATAAAGCAGGTCTGGCGGATGAGAAGGGGAAATTCCCGCGCATGAAGCa > 1:4398625/1‑53 (MQ=255) cGATAAAGCAGGTCTGGCGGATGAGAAGGGGAAATTCCCGCGCATGAAGCAGGCGCTGTATGTCGACAGt > 1:3216819/1‑70 (MQ=255) gATAAAGCAGGTCTGGCGGATGAGAAGGGGAAATTCCCGCGCATGAAGCAGGCGCTGTATGTCGACAGTAt > 1:2495491/1‑71 (MQ=255) cAGGTCTGGCGGATGAGAAGGGGAAATTCCCGCGCATGAAGCAGGCGCTGTATGTCGACAGTATCTCTTcc < 1:1747864/71‑1 (MQ=255) cAGGTCTGGCGGATGAGAAGGGGAAATTCCCGAGCATGAAGCAGGCGCTGTATGTCGACAGTATCTCtt > 1:3300094/1‑69 (MQ=255) cTGGCGGATGAGAAGGGGAAATTCCCGCGCATGAAGCAGGCGCTGTATGTCGACAGTATCTCtt > 1:1416196/1‑64 (MQ=255) gCGGATGAGAAGGGGAAATTCCCGCGTATGAAGCAGGCGCTGTATGTCGACAGTATCTCTTCCGTGACCg < 1:78061/70‑1 (MQ=255) ggATGAGAAGGGGAAATTCCCGCGCATGAAGCAGGCGCTGt < 1:3854239/41‑1 (MQ=255) ggATGAGAAGGGGAAATTCCCGCGCATGAAGCAGGCGCTGt < 1:2328224/41‑1 (MQ=255) gATGAGAAGGGGAAATTCCCGCGCATGAAGCAGGCGCTGTATGTCGACAGt < 1:2613933/51‑1 (MQ=255) gATGAGAAGGGGAAATTCCCGCGCATGAAGCAGGCGCTGTATGTCGACAGTATCTCTTCCGTGACCGGTTc > 1:637501/1‑71 (MQ=255) tGAGAAGGGGAAATTCCCGCGCATGAAGCAGGCGCTGTATGTCGACAGt > 1:4335847/1‑49 (MQ=255) agaAGGGGAAATTCCCGCGCATGAAGCAGGCGCTGTATGTCGACAGTATCTCTTCCGTGACCGGTTCGttt < 1:1256660/71‑1 (MQ=255) gaAGGGGAAATTCCCGCGCATGAAGCAGGCGCTGTATGTCGACAGTATCTCTTCCGTGACCGGTTCGTTTa > 1:239662/1‑71 (MQ=255) aaGGGGAAATTCCCGCGTATGAAGCAGGCGCTGTATGTCGACAGTATCTCTTCCGTGACCGGTTCg > 1:3425778/1‑66 (MQ=255) aaGGGGAAATTCCCGCGTATGAAGCAGGCGCTGTATGTCGACAGTATCTCTTCCGTGACCGGTTCGTTTAt > 1:4447137/1‑71 (MQ=255) gggAAATTCCCGCGCATGAAGCAGGCGCTGTATGTCGAc < 1:340382/39‑1 (MQ=255) gggAAATTCCCGCGCATGAAGCAGGCGCTGTATGTCGACAGTATCTCTTCCGTGACCGGTTCGTTTATCgg < 1:479027/71‑1 (MQ=255) gggAAATTCCCGCGCATGAAGCAGGCGCTGTATGTCGACAGTATCTCTTCCGTGACCGGTTCGTTTATCgg < 1:840623/71‑1 (MQ=255) gggAAATTCCCGCGCATGAAGCAGGCGCTGTATGTCGACAGTATCTCTTCCGTGACCGGTTCGTTTATCgg < 1:3447312/71‑1 (MQ=255) ggAAATTCCCGCGTATGAAGCAGGCGCTGTATGTCGACAGTATCTCTTCCGTGACCGGTTCGTTTATCgg < 1:579314/70‑1 (MQ=255) aaaTTCCCGCGTATGAAGCAGGCGCTGTATGTCGACAGTATCTCTTCCGTGAc < 1:1448883/53‑1 (MQ=255) aaTTCCCGCGCATGAAGCAGGCGCTGTATGTCGACAGTATCTCTTCCGTGACCg < 1:583814/54‑1 (MQ=255) ttCCCGCGCATGAAGCAGGCGCTGTATGTCGACAGt < 1:4605518/36‑1 (MQ=255) tCCCGCGCATGAAGCAGGCGCTGTATGTCGACAGTATCTCTTCCGTGACCGGTTCGTTTATCGGTActtct > 1:3391301/1‑71 (MQ=255) cccGCGTATGAAGCAGGCGCTGTATGTCGACAGTATCTCTTCCGTGACCGGTTCGTTTATCGGTActtctt > 1:1476402/1‑71 (MQ=255) cccGCGCATGAAGCAGGCGCTGTATGTCGACAGTATCTCTTCCGTGACCGGTTCGTTTATCGGTActtct < 1:2401514/70‑1 (MQ=255) cgcgTATGAAGCAGGCGCTGTATGTCGACAGTATCTCTTCCGTGACCGGTTCGTTTAt > 1:1152984/1‑58 (MQ=255) cgcgCATGAAGCAGGCGCTGTATGTCGACAGTATCTCTTCCGTGACCGGTTCGTTTATCGGTACTTCTTcc < 1:1286149/71‑1 (MQ=255) gcgcATGAAGCAGGCGCTGTATGTCGACAGTATCTCTTCCGTGACCGGTTCGTTTATCGGTACTTCTTCCg < 1:2826261/71‑1 (MQ=255) cgcATGAAGCAGGCGCTGTATGTCGACAGTATCTCTTCCGTGACCGGTTCGTTTATCGGTActtctt < 1:3695290/67‑1 (MQ=255) cgcATGAAGCAGGCGCTGTATGTCGACAGTATCTCTTCCGTGACCGGTTCGTTTATCGGTAc > 1:159187/1‑62 (MQ=255) cgcATGAAGCAGGCGCTGTATGTCGACAGTATCTCTTCCGTGACCGGTTCGTTTATCGGTACTTCTTCCGt < 1:2681422/71‑1 (MQ=255) cgcATGAAGCAGGCGCTGTATGTCGACAGTATCTCTTCCGTGACCGGTTCGTTTATCGGTACTTCTTCCGt < 1:227814/71‑1 (MQ=255) cgcATGAAGCAGGCGCTGTATGTCGACAGTATCTCTTCCGTGACCGGTTCGTTTATCGGTACTTCTTCCGt < 1:172845/71‑1 (MQ=255) | CTCCGGTACGCTGATTGGCGTGACCGATAAAGCAGGTCTGGCGGATGAGAAGGGGAAATTCCCGCGCATGAAGCAGGCGCTGTATGTCGACAGTATCTCTTCCGTGACCGGTTCGTTTATCGGTACTTCTTCCGT > W3110S.gb/3740877‑3741011 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |