| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,748,215 | G→A | 20.4% | S256S (AGC→AGT) | tnaB ← | tryptophan transporter of low affinity |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,748,215 | 0 | G | A | 20.4% | 95.0 / 18.0 | 44 | S256S (AGC→AGT) | tnaB | tryptophan transporter of low affinity |
| Reads supporting (aligned to +/- strand): ref base G (12/23); new base A (5/4); total (17/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01 | |||||||||||
CTGTTTGGTGCCGAGGAACGATTTCACCAGCGAATCAACGTTGCCGCCTGAGGAGATAATCGCCTTAAAGCTTTCTCGCGGAATATTCCCCATGGTGCAATAGAGCCAGAAGAGATAAATCACCAGCGCCAGCAGCG > W3110S.gb/3748146‑3748282 | cTGTTTGGTGCCGAGGAACGATTTCACCAGCGAATCAACGTTGCCGCCTGAGGAGATAATCGCCTTAAAGc > 1:3046756/1‑71 (MQ=255)cTGTTTGGTGCCGAGGAACGATTTCACCAGCGAATCAACGTTGCCGCCTGAGGAGATAATCGCCTTAAAGc > 1:4086143/1‑71 (MQ=255) tttGGTGCCGAGGAACGATTTCACCAGCGAATCAACGTTGCCGCCTGAGGAGATAATCGCCTTAAAGCt < 1:1376623/69‑1 (MQ=255) tttGGTGCCGAGGAACGATTTCACCAGCGAATCAACGTTGCCGCCTGAGGAGATAATCGCCTTAAAGCt < 1:547506/69‑1 (MQ=255) tttGGTGCCGAGGAACGATTTCACCAGCGAATCAACGTTGCCGCCTGAGGAGATAATCGCCTTAAAGCt < 1:2343686/69‑1 (MQ=255) tGCCGAGGAACGATTTCACCAGCGAATCAACGTTGCCGCCTGAGGAGATAATCGCCTTAAAGCTTTCTcgc < 1:827818/71‑1 (MQ=255) aTTTCACCAGCGAATCAACGTTGCCGCCTGAGGAGATAATCGCCTTAAAACTTTCTCGCGGaa < 1:1576345/63‑1 (MQ=255) tttCACCAGCGAATCAACGTTGCCGCCTGAGGAGATAATCGCCTTAAAGCTTTCTCGCGGa < 1:3371207/61‑1 (MQ=255) cACCAGCTAATCAACGTTGCCGCCTGAGGAGATAATCGCCTTAAAGCTTtctc < 1:2884630/53‑1 (MQ=255) aGCGAATCAACGTTGCCGCCTGAGGAGATAATCGCCTTAAAACTTTCTCGCGGAATATTCCCCATGGTGCa < 1:584877/71‑1 (MQ=255) gCGAATCAACGTTGCCGCCTGAGGAGATAATCGCCTTAAAGCTTTCTCGCgg < 1:4119567/52‑1 (MQ=255) gCGAATCAACGTTGCCGCCTGAGGAGATAATCGCCTTAAAGCTTTCTCGCGGAATATTCCCCATGGTGc < 1:1533704/69‑1 (MQ=255) cGAATCAACGTTGCCGCCTGAGGAGATAATCGCCTTAAAGCTTTCTcg < 1:2131018/48‑1 (MQ=255) gAATCAACGTTGCCGCCTGACGAGATAATCGCCTTAAAACTTTCTCGCGGAATATTCCCCATGGTGCa > 1:351535/1‑68 (MQ=255) aaTCAACGTTGCCGCCTGAGGAGATAATCGCCTTAAAGCTTTCTCGCGGAATATTCCCCATGGTGCAATag < 1:450649/71‑1 (MQ=255) aTCAACGTTGCCGCCTGAGGAGATAATCGCCTTAAAACTTTCTCGCGGAATATTCCCCATGGTGCAATaga < 1:2114748/71‑1 (MQ=255) aaCGTTGCCGCCTGAGGAGATAATCGCCTTAAAGCTTTCTCGCGGa < 1:4548276/46‑1 (MQ=255) aaCGTTGCCGCCTGAGGAGATAATCGCCTTAAAGCTTTCTCGCGGAata > 1:222797/1‑49 (MQ=255) aaCGTTGCCGCCTGAGGAGATAATCGCCTTAAAACTTTCTCGCGGAata > 1:2845099/1‑49 (MQ=255) aaCGTTGCCGCCTGAGGAGATAATCGCCTTAAAACTTTCTCGCGGAata > 1:1745955/1‑49 (MQ=255) cGTTGCCGCCTGAGGAGATAATCGCCTTAAAGCTTTCTCGCGGAATATTCCCCATGGTGCAATAGAGCCag < 1:1492785/71‑1 (MQ=255) ttGCCGCCTGAGGAGATAATCGCCTTAAAGCTTTCTCGCGGa < 1:520309/42‑1 (MQ=255) cTGAGGAGATAATCGCCTTAAAGCTTTCTCGCGGAATATTCCCCATGGTGCAATAGAGCCAGAAGAGATa > 1:2294745/1‑70 (MQ=255) aggagATAATCGCCTTAAAGCTTTCTCGCGGAATATTCCCCAt < 1:2767902/43‑1 (MQ=255) agaTAATCGCCTTAAAGCTTTCTCGCTGAATATTCCCCATGGTGCAATAGAGCCAGAAGAGATAAATCAcc < 1:2940758/71‑1 (MQ=255) gaTAATCGCCTTAAAGCTTTCTCGCGGAATATTCCCCATgg > 1:1132150/1‑41 (MQ=255) gaTAATCGCCTTAAAGCTTTCTCGCGGAATATTCCCCATGGTGCa < 1:3449172/45‑1 (MQ=255) gaTAATCGCCTTAAAGCTTTCTCGCGGAATATTCCCCATGGTGCa < 1:4411161/45‑1 (MQ=255) aTAATCGCCTTAAAGCTTTCTCGCGGAATATTCCCCATGGTGCAATagag < 1:452357/50‑1 (MQ=255) aTAATCGCCTTAAAGCTTTCTCGCGGAATATTCCCCATGGTGCAATAGAGCCAGAAGAGATAAATCACCAg > 1:1001785/1‑71 (MQ=255) aaTCGCCTTAAAACTTTCTCGCGGAATATTCCCCATGGTGCAATAGAGCCAGAAGAGATAAATCACCAgcg > 1:4341141/1‑71 (MQ=255) aaTCGCCTTAAAACTTTCTCGCGGAATATTCCCCATGGTG‑AATAGAGCCagaa > 1:3134656/1‑53 (MQ=255) tCGCCTTAAAGCTTTCTCGCGGAATATTCCCCATGGTGCAATAGAGCCAGAAGAGATaa < 1:617909/59‑1 (MQ=255) gCCTTAAAGCTTTCTCGCGGAATATTCCCCATGGTGCAATAGAGCCAGAAGAGATAAATCACCAGCGCcag > 1:2170220/1‑71 (MQ=255) gCCTTAAAACTTTCTCGCGGAATATTCCCCATGGTGCAATAGAGCCagaag < 1:975287/51‑1 (MQ=255) cTTAAAGCTTTCTCGCGGAATATTCCCCATGGTGCAATAGAGCCAGAAGAGATAAATc < 1:1870333/58‑1 (MQ=255) ttAAAGCTTTCTCGCGGAATATTCCCCATGGTGCAAt < 1:1541723/37‑1 (MQ=255) ttAAAGCTTTCTCGCGGAATATTCCCCATGGTGCAATaga < 1:1913121/40‑1 (MQ=255) tAAAGCTTTCTCGCGGAATATTCCCCATGGTGCAATa > 1:342982/1‑37 (MQ=255) tAAAGCTTTCTCGCGGAATATTCCCCATGGTGCAATAGAGCCAGAAgaga > 1:1527174/1‑50 (MQ=255) aaaGCTTTCTCGCGGAATATTCCCCATGGTGCAATAGAGCCAGAAGAGATaa > 1:2864468/1‑52 (MQ=255) aaaGCTTTCTCGCGGAATATTCCCCATGGTGCAATAGAGCCAGAAGAGATAAATCACCAGCGCCagcagc > 1:3867001/1‑70 (MQ=255) aaaGCTTTCTCGCGGAATATTCCCCATGGTGCAATAGAGCCAGAAGAGATAAATCACCAGCGCCagcagc > 1:2973687/1‑70 (MQ=255) aaGCTTTCTCGCGGAATATTCCCCATGGTGCAATAGAGCCAGAAGAGATAAATCACCAGCGCCAGCAGCg < 1:3816304/70‑1 (MQ=255) | CTGTTTGGTGCCGAGGAACGATTTCACCAGCGAATCAACGTTGCCGCCTGAGGAGATAATCGCCTTAAAGCTTTCTCGCGGAATATTCCCCATGGTGCAATAGAGCCAGAAGAGATAAATCACCAGCGCCAGCAGCG > W3110S.gb/3748146‑3748282 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |