| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,867,919 | C→T | 13.3% | G73D (GGC→GAC) | mtlA ← | fused mannitol‑specific PTS enzyme IIABC components |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,867,919 | 0 | C | T | 13.3% | 120.8 / 9.3 | 45 | G73D (GGC→GAC) | mtlA | fused mannitol‑specific PTS enzyme IIABC components |
| Reads supporting (aligned to +/- strand): ref base C (19/20); new base T (3/3); total (22/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.96e-01 | |||||||||||
GGCATGTCTGCGCCGACGATAACGCCCATGGTGGTGATGGCACCGACTACGCCGCCACGTTCGCCGCCTACCAGCTTACCACCGGTATAACCGATCAGCAGCGGCAGGAGATAAGTGATCATCGGCCCGA > W3110S.gb/3867853‑3867982 | ggCATGTCTGCGCCGACGATAACGCCCATGGTGGTGATGGCACCGACTACGCCGCCACGTTCGCCGCCTa > 1:899539/1‑70 (MQ=255)ggCATGTCTGCGCCGACGATAACGCCCATGGTGGTGATGGCACCGACTACGCCGCCACGTTCGCCGCCTAc < 1:4415369/71‑1 (MQ=255)ggCATGTCTGCGCCGACGATAACGCCCATGGTGGTGATGGCACCGACTACGCCGCCACGTTCGCCGCCTAc > 1:3619392/1‑71 (MQ=255) tGTCTGCGCCGACGATAACGCCCATGGTGGTGATGGCACCGACTACGCCGCCACGTTCGCCGCCTACCAGc < 1:1310315/71‑1 (MQ=255) tCTGCGCCGACGATAACGCCCATGGTGGTGATGGCACCGACTACGCCGCCACGTTCGCCGTCTACCAGCt > 1:2935710/1‑70 (MQ=255) tCTGCGCCGACGATAACGCCCATGGTGGTGATGGCACCGACTACGCCGCCACGTTCGCCGCCTACCAGCt > 1:3418846/1‑70 (MQ=255) cgcCGACGATAACGCCCATGGTGGTGATGGCACCGACTACGCCGCCACGTTCGCCGCCTACCAGCTTacca > 1:3261211/1‑71 (MQ=255) gaTAACGCCCATGGTGGTGATGGCACCGACTACGCCGCCACGTTCGCCGCCTACCAGCTTACCACCGGta > 1:488213/1‑70 (MQ=255) gaTAACGCCCATGGTGGTGATGGCACCGACTACGCCGCCACGTTCGCCGCCTAACAGCTTACCACCGGta > 1:1021574/1‑70 (MQ=255) aTAACGCCCATGGTGGTGATGGCACCGACTACGCCGCCACGTTCGCCGCCTACCAGCTTACCACCGGtata < 1:2614376/71‑1 (MQ=255) aCGCCCATGGTGGTGATGGCACCGACTACGCCGCCACGTTCGCCTCCTACCAGCTTACCACCGGtat < 1:2890891/67‑1 (MQ=255) aCGCCCATGGTGGTGATGGCACCGACTACGCCGCCACGTTCGCCGCCTACCAGCTTACCACCGGtat < 1:4153177/67‑1 (MQ=255) aCGCCCATGGTGGTGATGGCACCGACTACGCCGCCACGTTCGCCGCCTACCAGCTTACCACCGGTATAAcc < 1:2217725/71‑1 (MQ=255) aCGCCCATGGTGGTGATGGCACCGACTACGCCGCCACGTTCGCCGCCTACCAGCTTACCACCGGTATAAcc < 1:4511944/71‑1 (MQ=255) aTGGTGGTGATGGCACCGACTACGCCGCCACGTTCGCCGCCTACCAGCTTACCACCGGTATAACCGATcag > 1:4301094/1‑71 (MQ=255) tggtggTGATGGCACCGACTACGCCGCCACGTTCGCCGCCTACCAGCTTACCACCGGTATAACCGATcagc < 1:2381653/71‑1 (MQ=255) gtgATGGCACCGACTACGCCGCCACGTTCGCCGCCTACCa < 1:3981104/40‑1 (MQ=255) gATGGCACCGACTACGCCGCCACGTTCGCCGCCTACCAGCTTACCACCgg > 1:2687956/1‑50 (MQ=255) ggCACCGACTACGCCGCCACGTTCGCCGCCTACCAGCTTACCACCGGTATAACCGATCAGCAGCGGCAg > 1:4344385/1‑69 (MQ=255) ggCACCGACTACGACGCCACGTTCGCCGCCTACCAGCTTACCACCGGTATAACCGATCAGCAGCGGCAg > 1:2925455/1‑69 (MQ=255) gCACCGACTACGCCGCCACGTTCGCCGCCTACCAGCTTACCACCGGta > 1:2039956/1‑48 (MQ=255) gCACCGACTACGCCGCCACGTTCGCCGCCTACCAGCTTACCACCGGTATAACCGATCAGCAGCGGCAg > 1:3138195/1‑68 (MQ=255) gCACCGACTACGCCGCCACGTTCGCCGCCTACCAGCTTACCACCGGTATAACCGATCAGCAGCGGCAg > 1:3668968/1‑68 (MQ=255) cACCGACTACGCCGCCACGTTCGCCGCCTACCAGCTTACCACCGGTATAACCGATCAGCAGCGGCAGgaga < 1:4581809/71‑1 (MQ=255) aCCGACTACGCCGCCACGTTCGCCGCCTACCAGCTTACCACCGGTATAACCGATCAGCAGCGGCAGgaga > 1:625069/1‑70 (MQ=255) aCCGACTACGCCGCCACGTTCGCCGCCTACCAGCTTACCACCGGTATAACCGATCAGCAGCGGCAGGAGAt < 1:3104730/71‑1 (MQ=255) ccGACTACGCCGCCACGTTCGCCGCCTACCAGCTTACCACCGGTATAACCGATCAGCAGC‑‑CAGGAGATAAg > 1:277217/1‑71 (MQ=255) gACTACGCCGCCACGTTCGCCGCCTACCAGCTTACCACCGGTATAACCGATCAGCAGCGGCAGGAGATatg < 1:3946511/71‑3 (MQ=255) gACTACGCCGCCACGTTCGCCGCCTACCAGCTTACCACCGGTATAACCGATCAGCAGCGGCAGGAGATAAg > 1:4318763/1‑71 (MQ=255) aCTACGCCGCCACGTTCGCCGTCTACCAGCTTACCACCGGTATAACCGATCAGCAGCGGCAGGAGATAAGt < 1:2964918/71‑1 (MQ=255) aCTACGCCGCCACGTTCGCCGTCTACCAGCTTACCACCGGTATAACCGATCAGCAGCGGCAGGAGATAAGt < 1:2970178/71‑1 (MQ=255) aCTACGCCGCCACGTTCGCCGCCTACCAGCTTACCACCGGTATAACCGATCAGCAGCGGCAGGAGATAAGt > 1:3157760/1‑71 (MQ=255) cTACGCCGCCACGTTCGCCGCCTACCAGCTTACCACCGGTATAACCGATCAGCAGCGGCAGGAGATAAGt > 1:2759005/1‑70 (MQ=255) cTACGCCGCCACGTTCGCCGCCTACCAGCTTACCACCGGTATAACCGATCAGCAGCGGCAGGAGATAAGTg < 1:1150121/71‑1 (MQ=255) cTACGCCGCCACGTTCGCCGCCTACCAGCTTACCACCGGTATAACCGATCAGCAGCGGCAGGAGATAAGTg < 1:3182901/71‑1 (MQ=255) cTACGCCGCCACGTTCGCCGCCTACCAGCTTACCACCGGTATAACCGATCAGCAGCGGCAGGAGATAAGTg < 1:164526/71‑1 (MQ=255) aCGCCGCCACGTTCGCCGCCTACCAGCTTACCACCGGtata < 1:4256462/41‑1 (MQ=255) cgccgcCACGTTCGCCGCCTACCAGCTTACCACCGGTATAACCGATCAGCAGCGGCAGGAGATAAGTGa < 1:555040/69‑1 (MQ=255) ccgccACGTTCGCCGCCTACCAGCTTACCACCGGTATAACCGATCAGCAGCGGCAGGAGATAAGTGatcat < 1:4114823/71‑1 (MQ=255) ccACGTTCGCCGCCTACCAGCTTACCACCGGTATAACCGATCAGCAGCGGCAGGAGAt < 1:4401253/58‑1 (MQ=255) cACGTTCGCCGCCTACCAGCTTACCACCGGTATAACCGATCAGCAGCGGCAGGAGATAAGTg < 1:954626/62‑1 (MQ=255) aCGTTCGCCGTCTACCAGCTTACCACCGGTATAACCGATCAGCAGCGGCAGGAGATAAGTGATCATCGGcc > 1:3686063/1‑71 (MQ=255) aCGTTCGCCGTCTACCAGCTTACCACCGGTATAACCGATCAGCAGCGGCAGGAGATAAGTGATCATCGGcc > 1:2686166/1‑71 (MQ=255) cGTTCGCCGTCTACCAGCTTACCACCGGTATAACCGATCAGCAGCGGCAGGAGATAAGTGa < 1:3829543/61‑1 (MQ=255) ttCGCCGCCTACCAGCTTACCACCGGTATAACCGATCAGCAGCGGCAGGAGATAAGTGATCATCGGCCCGa > 1:1501989/1‑71 (MQ=255) | GGCATGTCTGCGCCGACGATAACGCCCATGGTGGTGATGGCACCGACTACGCCGCCACGTTCGCCGCCTACCAGCTTACCACCGGTATAACCGATCAGCAGCGGCAGGAGATAAGTGATCATCGGCCCGA > W3110S.gb/3867853‑3867982 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |