| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,871,558 | T→C | 12.6% | intergenic (+1384/+221) | yibH → / ← yibG | hypothetical protein/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,871,558 | 0 | T | C | 12.6% | 252.6 / 25.9 | 100 | intergenic (+1384/+221) | yibH/yibG | hypothetical protein/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (30/57); new base C (9/3); total (40/60) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.07e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01 | |||||||||||
Only 100 of 102 total aligned reads displayed.
AGCGACTGGAATACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAGTTATGCTGTATAAACAGGTGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACG > W3110S.gb/3871490‑3871627 | aGCGACTGGAATACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCAt < 1:3369156/69‑1 (MQ=255) aCTGGAATACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTaa < 1:2729565/71‑1 (MQ=255) aCTGGAATACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTa < 1:3380013/70‑1 (MQ=255) aCTGGAATACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTa < 1:4489906/70‑1 (MQ=255) gAATACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAGt < 1:3232027/70‑1 (MQ=255) cTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAGTTATGCt > 1:1739430/1‑71 (MQ=255) cTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAGTTATGCt < 1:361493/71‑1 (MQ=255) cTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAGTTATGCt > 1:3857693/1‑71 (MQ=255) cTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAGTTATGCt > 1:2083586/1‑71 (MQ=255) cTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAGTTATGCt > 1:4322973/1‑71 (MQ=255) cTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAGTTATGCt < 1:1247859/71‑1 (MQ=255) aGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAGTTATGCTGta > 1:1246863/1‑71 (MQ=255) aGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAGTTATGCTGta < 1:4589469/71‑1 (MQ=255) aGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAGTTATGCTGta > 1:1671764/1‑71 (MQ=255) aGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCACACCTAAAGTTATGCTGta > 1:2656392/1‑71 (MQ=255) aGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCACACCTAAAGTTATGCTGta > 1:3657913/1‑71 (MQ=255) acaTCATAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAGTTATGCTGTATaa < 1:1001314/71‑1 (MQ=255) catcatAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAGTTATGCTGTATaaa < 1:4247565/71‑1 (MQ=255) atcatAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAGTTATGCTg < 1:2532583/64‑1 (MQ=255) atcatAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAGTTATGCTg < 1:2634782/64‑1 (MQ=255) atcatAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAGTTATGCTGtata < 1:3050307/68‑1 (MQ=255) atcatAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAGTTATGCTGTATaa < 1:421726/69‑1 (MQ=255) atcatAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAGTTATGCTGTATAAAc < 1:3183516/71‑1 (MQ=255) tcatAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAGTTATGCTGTATAAACa < 1:3363017/71‑1 (MQ=255) tcatAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAGTTATGCTGTATAAACa < 1:4236132/71‑1 (MQ=255) tcatAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAGTTATGCTGTATAAACa < 1:1619970/71‑1 (MQ=255) tcatAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAGTTATGCTGTATAAACa < 1:3099585/71‑1 (MQ=255) tcatAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAGTTATGCTGTATAAACa < 1:936733/71‑1 (MQ=255) atAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAGTTATGCTGTATAAACa > 1:286930/1‑69 (MQ=255) atAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAGTTATGCTGTATAAACAgg > 1:3743246/1‑71 (MQ=255) atAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAGTTATGCTGTATAAACAg < 1:4001751/70‑1 (MQ=255) atAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAGTTATGCTGTATAAACAg < 1:1689664/70‑1 (MQ=255) tAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAGt < 1:2090896/53‑1 (MQ=255) tAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAGTTATGCTGtata < 1:2097744/64‑1 (MQ=255) aaaaaTTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAGTTATGCTGTATAAACAgg > 1:3035041/1‑69 (MQ=255) aaaaTTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAGt < 1:1095385/51‑1 (MQ=255) aaaaTTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAGTTATGCTGTATAAACAGGTgg > 1:1470888/1‑71 (MQ=255) aaaaTTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAGTTATGCTGTATAAACAGGTg < 1:4009885/70‑1 (MQ=255) aTTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAGTTATGCTGTATAAACAGGTGGCt < 1:1895865/70‑1 (MQ=255) aTTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAGTTATGCTGTATAAACAGGTGGCTa < 1:4140518/71‑1 (MQ=255) aTTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAGTTATGCTGTATAAACAGGTGGCTa < 1:4345454/71‑1 (MQ=255) ttATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAGt < 1:2512040/47‑1 (MQ=255) ttATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAGTTATGCTGTATAAACAGGTGGCTaa < 1:3430118/71‑1 (MQ=255) ttATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAGTTATGCTGTATAAACAGGTGGCTa < 1:759212/70‑1 (MQ=255) atcatcACATGGTATATCTGAAACACACGAAAGCATACCTAAAGTTATGCTGtata < 1:842729/56‑1 (MQ=255) atcaCATGGTATATCTGAAACACACGAAAGCATACCTAAAGTTATGCTGTATAAACAGGTGGCTAATCCCa < 1:1766318/71‑1 (MQ=255) tcaCATGGTATATCTGAAACACACGAAAGCATACCTAAAGTTATGCTGTATAAACAGGTGGCTAATccc > 1:2165980/1‑69 (MQ=255) acaTGGTATATCTGAAACACACGAAAGCATACCTAAAGTTATg < 1:4214559/43‑1 (MQ=255) acaTGGTATATCTGAAACACACGAAAGCATACCTAAAGTTATGCTGt > 1:3957004/1‑47 (MQ=255) acaTGGTATATCTGAAACACACGAAAGCATACCTAAAGTTATGCTGt > 1:3962388/1‑47 (MQ=255) acaTGGTATATCTGAAACACACGAAAGCACACCTAAAGTTATGCTGTATAAACAGGTGGCTAATCCCAAgg > 1:3979073/1‑71 (MQ=255) acaTGGTATATCTGAAACACACGAAAGCACACCTAAAGTTATGCTGTATAAACAGGTGGCTAATCCCAAgg > 1:2175511/1‑71 (MQ=255) ggTATATCTGAAACACACGAAAGCATACCTAAAGTTATGCTGTATAAACAGGTGGCTAATCCCAAGGGGAt > 1:2055577/1‑71 (MQ=255) tataTCTGAAACACACGAAAGCATACCTAAAGTTATGCTGTATAAACAGGTGGCTaa < 1:2025532/57‑1 (MQ=255) tatCTGAAACACACGAAAGCATACCTAAAGTTATGCTg < 1:2331928/38‑1 (MQ=255) tatCTGAAACACACGAAAGCATACCTAAAGTTATGCTGtata < 1:2482158/42‑1 (MQ=255) tatCTGAAACACACGAAAGCATACCTAAAGTTATGCTGtata < 1:1472706/42‑1 (MQ=255) tatCTGAAACACACGAAAGCATACCTAAAGTTATGCTGtata < 1:3589486/42‑1 (MQ=255) tatCTGAAACACACGAAAGCATACCTAAAGTTATGCTGTATAAACAGGTGGCTAATCCCAAgggg < 1:3025067/65‑1 (MQ=255) tatCTGAAACACACGAAAGCATACCTAAAGTTATGCTGTATAAACAGGTGGCTAATCCCAAgggg < 1:3640499/65‑1 (MQ=255) tatCTGAAACACACGAAAGCACACCTAAAGTTATGCTGTATAAACAGGTGGCTAATCCCAAGGGGATCGAt < 1:785658/71‑1 (MQ=255) tatCTGAAACACACGAAAGCACACCTAAAGTTATGCTGTATAAACAGGTGGCTAATCCCAAGGGGATCGAt < 1:769363/71‑1 (MQ=255) atCTGAAACACACGAAAGCATAGCTAAAGTTATGCTg < 1:517041/37‑1 (MQ=255) atCTGAAACACACGAAAGCATACCTAAAGTTATGCTGTATaaa < 1:38111/43‑1 (MQ=255) tatGAAACACACGAAAGCATACCTAAAGTTATGCTGTATAAACAGGTGGCTAATCCCAAGGGGATCGATTc > 1:4014651/3‑71 (MQ=255) tCTGAAACACACGAAAGCATACCTAAAGTTATGCTGTATAAACAgg > 1:2961353/1‑46 (MQ=255) tCTGAAACACACGAAAGCATACCTAAAGTTATGCTGTATAAACAg > 1:1583138/1‑45 (MQ=255) tCTGAAACACACGAAAGCATACCTAAAGTTATGCTGTATAAACAGGTg < 1:1148752/48‑1 (MQ=255) tCTGAAACACACGAAAGCATACCTAAAGTTATGCTGTATAAACAGGTg < 1:346616/48‑1 (MQ=255) tCTGAAACACACGAAAGCATACCTAAAGTTATGCTGTATAAACAGGTGGCTAATCCCAAGGGGAt > 1:4284029/1‑65 (MQ=255) tCTGAAACACACGAAAGCATACCTAAAGTTATGCTGTATAAACAGGTGGCTAATCCCAAGGGGATCGa > 1:474111/1‑68 (MQ=255) tCTGAAACACACGAAAGCATACCTAAAGTTATGCTGTATAAACAGGTGGCTAATCCCAAGGGGATCGATTc > 1:4344380/1‑71 (MQ=255) tCTGAAACACACGAAAGCATACCTAAAGTTATGCTGTATAAACAGGTGGCTAATCCCAAGGGGATCGATTc > 1:1939517/1‑71 (MQ=255) tCTGAAACACACGAAAGCATACCTAAAGTTATGCTGTATAAACAGGTGGCTAATCCCAAGGGGATCGATTc > 1:2125867/1‑71 (MQ=255) tCTGAAACACACGAAAGCATACCTAAAGTTATGCTGTATAAACAGGTGGCTAATCCCAAGGGGATCGATTc > 1:4260921/1‑71 (MQ=255) tCTGAAACACACGAAAGCACACCTAAAGTTATGCTGTATAAACAGGTGGCt < 1:1353579/51‑1 (MQ=255) cTGAAACACACGAAAGCATACCTAAAGTTATGCTGTATAAACAGGTGGCTAATCCCAAGGGGATCGATTc > 1:4217529/1‑70 (MQ=255) tGAAACACACGAAAGCACACCTAAAGTTATGCTGTATAAACAGGTGGCTaa > 1:368379/1‑51 (MQ=255) aaaCACACGAAAGCATACCTAAAGTTATGCTGtata < 1:2656880/36‑1 (MQ=255) aaCACACGAAAGCATACCTAAAGTTATGCTGTATAAACAGGTGGCTAATCCCAAGGGGATCGATTCCAGaa < 1:3940311/71‑1 (MQ=255) acacacGAAAGCATACCTAAAGTTATGCTGTATAAACAGGTGGCTAATCCCAAGGGGATCGATTCCAGaaa > 1:2518617/1‑71 (MQ=255) acacacGAAAGCATACCTAAAGTTATGCTGTATAAACAGGTGGCTAATCCCAAGGGGATCGATTCCAGaaa > 1:3388447/1‑71 (MQ=255) acacacGAAAGCATACCTAAAGTTATGCTGTATAAACAGGTGGCTAATCCCAAGGGGATCGATTCCAGaa < 1:1344406/70‑1 (MQ=255) cacGAAAGCATACCTAAAGTTATGCTGTATAAACAGGTGGCTAATCCCAAGGGGATCGATTCCAGAAATCg < 1:1434150/71‑1 (MQ=255) acGAAAGCATACCTAAAGTTATGCTGTATAAACAGGTGGCTAATccc < 1:565123/47‑1 (MQ=255) cGAAAGCATACCTAAAGTTATGCTGTATAAACAGGTGGCTAATCCCAAGGGGATCg > 1:3229284/1‑56 (MQ=255) cGAAAGCACACCTAAAGTTATGCTGTATAAACAgg > 1:2313201/1‑35 (MQ=255) cGAAAGCACACCTAAAGTTATGCTGTATAAACAgg > 1:4357262/1‑35 (MQ=255) cGAAAGCACACCTAAAGTTATGCTGTATAAACAGGTGGCTAATCCCAAGGGGATCGATTcaa > 1:2422127/1‑60 (MQ=255) cGAAAGCAAACCTAAAGTTATGCTGTATAAACAgg > 1:3879443/1‑35 (MQ=255) gAAAGCATACCTAAAGTTATGCTGTATAAACAGGTGGCTAATCCCaa > 1:4320130/1‑47 (MQ=255) gAAAGCATACCTAAAGTTATGCTGTATAAACAGGTGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGt > 1:2425295/1‑71 (MQ=255) aaGCATACCTAAAGTTATGCTGTATAAACAGGTGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTc < 1:1307912/71‑1 (MQ=255) aaGCATACCTAAAGTTATGCTGTATAAACAGGTGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTc < 1:2470639/71‑1 (MQ=255) aaGCATACCTAAAGTTATGCTGTATAAACAGGTGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTc < 1:4360081/71‑1 (MQ=255) aGCATACCTAAAGTTATGCTGTATAAACAGGTGGCTAATCCCAAGGGGATCGATTCCAGaaa > 1:3903060/1‑62 (MQ=255) aGCATACCTAAAGTTATGCTGTATAAACAGGTGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCa < 1:1195849/71‑1 (MQ=255) aTACCTAAAGTTATGCTGTATAAACAGGTGGCTAATCCCAAGGGGATCGATTCCAGAAATCTGGTTCAACg < 1:2771321/71‑1 (MQ=255) aTACCTAAAGTTATGCTGTATAAACAGGTGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACg < 1:3325209/71‑1 (MQ=255) tACCTAAAGTTATGCTGTATAAACAGGTGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTc < 1:1685418/66‑1 (MQ=255) | AGCGACTGGAATACTAAGCACATCATAAAAATTATCATCACATGGTATATCTGAAACACACGAAAGCATACCTAAAGTTATGCTGTATAAACAGGTGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACG > W3110S.gb/3871490‑3871627 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |