| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,903,549 | T→C | 6.1% | S104P (TCA→CCA) | bax → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,903,549 | 0 | T | C | 6.1% | 229.4 / 5.6 | 82 | S104P (TCA→CCA) | bax | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (37/40); new base C (2/3); total (39/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01 | |||||||||||
TCTCCGGACCGTAATGCCTTACATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGACATCGCCAAACGCTACA > W3110S.gb/3903479‑3903618 | tctcCGGACCGTAATGCCTTACATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCAttt > 1:919335/1‑71 (MQ=255)tctcCGGACCGTAATGCCTTACATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCAttt > 1:1997771/1‑71 (MQ=255)tctcCGGACCGTAATGCCTTACATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCAttt > 1:906040/1‑71 (MQ=255)tctcCGGACCGTAATGCCTTACATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCAttt > 1:670880/1‑71 (MQ=255)tctcCGGACCGTAATGCCTTACATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCAttt > 1:1510511/1‑71 (MQ=255) cGGACCGTAATGCCTTACATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCaa < 1:364595/70‑1 (MQ=255) cGTAATGCCTTACATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGt < 1:2049271/71‑1 (MQ=255) gTAATGCCTTACATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTa < 1:927646/71‑1 (MQ=255) tAATGCCTTACATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTAt > 1:1250926/1‑71 (MQ=255) tAATGCCTTACATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTAt > 1:83376/1‑71 (MQ=255) tAATGCCTTACATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTAt < 1:2383503/71‑1 (MQ=255) tAATGCCTTACATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTAt > 1:1466378/1‑71 (MQ=255) aTGCCTTACATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTAt > 1:4334128/1‑69 (MQ=255) aTGCCTTACATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATc < 1:1514345/70‑1 (MQ=255) tGCCTTACATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAg > 1:2633522/1‑71 (MQ=255) ccTTACATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAggg > 1:3780412/1‑71 (MQ=255) tACATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGcc > 1:2092454/1‑70 (MQ=255) aCATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGcc < 1:2720084/69‑1 (MQ=255) aCATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCaa > 1:3680465/1‑71 (MQ=255) aCATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCaa > 1:2235293/1‑71 (MQ=255) aCATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCaa > 1:2190584/1‑71 (MQ=255) aCATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCa < 1:4216643/70‑1 (MQ=255) aTTACCAGGCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATg > 1:803723/1‑71 (MQ=255) ttACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCaa < 1:1236969/51‑1 (MQ=255) ttACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAg > 1:3293433/1‑56 (MQ=255) ttACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAg > 1:993954/1‑56 (MQ=255) ttACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTa < 1:1718065/58‑1 (MQ=255) ttACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAgg < 1:4424586/63‑1 (MQ=255) tACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGt > 1:2612520/1‑71 (MQ=255) aCCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGt > 1:4427608/1‑70 (MQ=255) ccAGCCAAAATGCGGCCTTTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTc < 1:2868951/70‑1 (MQ=255) ccAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTc < 1:4048839/70‑1 (MQ=255) ccAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTc < 1:182282/70‑1 (MQ=255) ccAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTc < 1:617236/70‑1 (MQ=255) ccAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTCCAAAACAGTATCAGGTCCAATGGTc < 1:1432855/70‑1 (MQ=255) gCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACct > 1:747502/1‑71 (MQ=255) ccAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACctg < 1:2358213/71‑1 (MQ=255) aaaaTGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACa > 1:1138176/1‑46 (MQ=255) aaaaTGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCa < 1:4631220/52‑1 (MQ=255) aaaaTGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACctgct > 1:3253804/1‑71 (MQ=255) aaaaTGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACctgct > 1:1954454/1‑71 (MQ=255) aaaaTGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACctgct < 1:549475/71‑1 (MQ=255) aaaaTGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACctgct > 1:252831/1‑71 (MQ=255) aaaaTGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACctgct > 1:2307343/1‑71 (MQ=255) aTGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAg < 1:205622/71‑1 (MQ=255) ggCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGt < 1:2226023/70‑1 (MQ=255) ggCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTg < 1:3569830/71‑1 (MQ=255) ttACTGCGGAACGTAACTGGCTCATTTCAAAACAGTa < 1:2396542/37‑1 (MQ=255) ttACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAgg < 1:1112645/42‑1 (MQ=255) ttACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGc < 1:2281316/44‑1 (MQ=255) ttACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTc < 1:1584617/52‑1 (MQ=255) ttACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGt < 1:3731667/71‑1 (MQ=255) aCTGCGGAACGTAACTGGCTCATTTCAAAACAGTAt > 1:3376358/1‑36 (MQ=255) aCTGCGGAACGTAACTGGCTCATTTCAAAACAGTATc > 1:4054549/1‑37 (MQ=255) aCTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGa > 1:3680926/1‑59 (MQ=255) aCTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCt > 1:201295/1‑71 (MQ=255) aCTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGG‑CAATGGTCACCTGCTGa > 1:2999342/1‑58 (MQ=255) aCTGCGGAACGTAACTGGCTCATTCCAAAACAGTAt > 1:208398/1‑36 (MQ=255) tGCGGAACGTAACTGGCTCATTTCAAAACAGTATc < 1:1835727/35‑1 (MQ=255) tGCGGAACGTAACTGGCTCATTTCAAAACAGTATc < 1:1264655/35‑1 (MQ=255) tGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAg < 1:709811/58‑1 (MQ=255) tGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCg < 1:1994294/60‑1 (MQ=255) cGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGaaa < 1:474255/71‑1 (MQ=255) cGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGa > 1:1291753/1‑69 (MQ=255) cGGAACGTAACTGGCTCATTCCAAAACAGTATCAGGGCCaa > 1:1261686/1‑41 (MQ=255) cGGAACGTAACTGGCTCATTCCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGaaa < 1:2237541/71‑1 (MQ=255) ggAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAg < 1:2328160/71‑1 (MQ=255) gAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGa > 1:4227988/1‑71 (MQ=255) aaCGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTc < 1:923511/43‑1 (MQ=255) aaCGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGAc < 1:537794/71‑1 (MQ=255) cGTAACTGGCTCATTTCAAAACAGTATCAGGGCCaa > 1:1926152/1‑36 (MQ=255) cGTAACTGGCTCATTTCAAAACAGTATCAGGGCCaa > 1:1082823/1‑36 (MQ=255) cGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTc < 1:3906714/41‑1 (MQ=255) cGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTg < 1:1639595/63‑1 (MQ=255) cGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTg < 1:2045420/63‑1 (MQ=255) cGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGACa > 1:4378963/1‑70 (MQ=255) cGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGACAt > 1:3466983/1‑71 (MQ=255) gTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGa > 1:2819281/1‑49 (MQ=255) gTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGa > 1:3347673/1‑49 (MQ=255) aCTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACctg > 1:2166198/1‑42 (MQ=255) cTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTc < 1:3695020/36‑1 (MQ=255) cTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGACAt > 1:575808/1‑66 (MQ=255) cTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGACATCGCCa < 1:13078/71‑1 (MQ=255) tGGCTCATTCCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGa < 1:3157165/58‑1 (MQ=255) ggCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGACATCGCCaaa > 1:2924157/1‑71 (MQ=255) gCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGACATCGCCaaa < 1:925276/70‑1 (MQ=255) aTTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGACATCGCCAAACGCt < 1:4491989/70‑1 (MQ=255) tttCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAg < 1:2568516/38‑1 (MQ=255) tttCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGa < 1:2033637/51‑1 (MQ=255) tttCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGACATCGCCAAACGCTAc < 1:3825323/71‑1 (MQ=255) ttCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTg > 1:1045541/1‑41 (MQ=255) ttCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCTTCTGAAAGACATCGCCAAACGCt < 1:1820160/68‑1 (MQ=255) ttCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGa > 1:207079/1‑54 (MQ=255) ttCAAAACAGTATCAGGGCCAATGGTCACATGCTGAGCGTg > 1:1403384/1‑41 (MQ=255) tCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGa > 1:3978833/1‑53 (MQ=255) tCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGACATCGCCAAACGCTAc > 1:1453199/1‑69 (MQ=255) tCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGACATCGCCAAACGCTACa < 1:1470515/70‑1 (MQ=255) tCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGACATCGCCAAACGCTACa < 1:2093528/70‑1 (MQ=255) | TCTCCGGACCGTAATGCCTTACATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGACATCGCCAAACGCTACA > W3110S.gb/3903479‑3903618 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |