| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,916,897 | A→G | 10.6% | N293S (AAC→AGC) | glyS → | glycine tRNA synthetase, beta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,916,897 | 0 | A | G | 10.6% | 119.1 / 6.9 | 47 | N293S (AAC→AGC) | glyS | glycine tRNA synthetase, beta subunit |
| Reads supporting (aligned to +/- strand): ref base A (22/20); new base G (3/2); total (25/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01 | |||||||||||
GGCTGAAGCGCTGGTTTACACCATGAAAGGTGACCAGAAATACTTCCCGGTGTATGCGAACGACGGCAAACTGCTGCCGAACTTTATCTTCGTTGCCAACATCGAATCGAAAGATCCGCAGCAGATTATC > W3110S.gb/3916838‑3916967 | ggCTGAAGCGCTGGTTTACACCATGAAAGGTGACCAGAAATACTTCCCGGTGTATGCGAACGACGGCAAAc < 1:1427340/71‑1 (MQ=255) gCTGAAGCGCTGGTTTACACCATGAAAGGTGACCAGAAATACTTCCCGGTGTATGCGAACGACGGCAAAct > 1:4589604/1‑71 (MQ=255) cgcTGGTTTACACCATGAAAGGTGACCAGAAATACTTCCCGGTGTATGCGAACGACGGCAAACTGCTGCCg < 1:1224836/71‑1 (MQ=255) gTTTACACCATGAAAGGTGACCAGAAATACTTCCCGGTGTATGCGAACGACgg < 1:3039313/53‑1 (MQ=255) gTTTACACCATGAAAGGTGACCAGAAATACTTCCCGGTGTATGCGAACGACGGCa < 1:2105702/55‑1 (MQ=255) cacCATGAAAGGTGACCAGAAATACTTCCCGGTGTATGCGAac < 1:2378437/43‑1 (MQ=255) cacCATGAAAGGTGACCAGAAATACTTCCCGGTGTATGCGAACGACGGCaaa > 1:4093399/1‑52 (MQ=255) cATGAAAGGTGACCAGAAATACTTCCCGGTGTATGCGAGCGACGGCAAACTGCTGCCGAACTTTATCTTCg > 1:355050/1‑71 (MQ=255) aTGAAAGGTGACCAGAAATACTTCCCGGTGTATGCGAACGACGGCAAACTGCTGCCGAACTTTATCTTCGt < 1:2334317/71‑1 (MQ=255) gAAAGGTGACCAGAAATACTTCCCGGTGTATGCGAACGACGGCAAACTGCTGCCGAACt < 1:764811/59‑1 (MQ=255) aaaGGTGACCAGAAATACTTCCCGGTGTATGCGAACGACGGCAAACTGCTGCCGAACTTTATCTTCGTTGc > 1:3385880/1‑71 (MQ=255) aaGGTGACCAGAAATACTTCCCGGTGTATGCGAACGACGGCAAACTGCTGCCGAACTTTATCTTCGTTGcc < 1:894419/71‑1 (MQ=255) aGGTGACCAGAAATACTTCCCGGTGTATGCGAGCGACGGCAAACTGCTGCCGaa > 1:2862486/1‑54 (MQ=255) ggTGACCAGAAATACTTCCCGGTGTATGCGAACGACGGCAAACTGCTGCCGAACTTTATCTTCGTTGCCaa < 1:4498732/71‑1 (MQ=255) tGACCAGAAATACTTCCCGGTGTATGCGAACGACGGCAAACTGCTGCCGAACTTTAt > 1:294560/1‑57 (MQ=255) ccAGAAATACTTCCCGGTGTATGCGAACGACGGCAAACTGCTGCCGAACTTTATCTTCGTTGCCAACatcg < 1:2783459/71‑1 (MQ=255) cAGAAATACTTCCCGGTGTATGCGAGCGACGGCAAACTGCTGCCGAACTTTATCTTCGATGCCAACatcg > 1:3179096/1‑70 (MQ=255) aGAAATACTTCCCGGTGTATGCGAACGACGGCAAACTGCTGCCGAACTTTATCTTCGTTGCCAACatcgaa < 1:886757/71‑1 (MQ=255) gAAATACTTCCCGGTGTATGCGAACGACGGCAAACTGCTGCCGAACttt < 1:885515/49‑1 (MQ=255) aaaTACTTCCCGGTGTATGCGAGCGACGGCAAACTGCTGCCGAACTTTATCTTCGTTGCCAACatcgaatc < 1:1873795/71‑1 (MQ=255) aTACTTCCCGGTGTATGCGAACGACGGCAAACTGCTGCCGAACTTTATc > 1:1881349/1‑49 (MQ=255) aTACTTCCCGGTGTATGCGAACGACGGCAAACTGCTGCCGAACTTTATCTTCGTTg > 1:548243/1‑56 (MQ=255) aTACTTCCCGGTGTATGCGAACGACGGCAAACTGCTGCCGAACTTTATCTTCGTTg > 1:3621000/1‑56 (MQ=255) aTACTTCCCGGTGTATGCGAACGACGGCAAACTGCTGCCGAACTTTATCTTCGTTGCCAACatcgaatcg > 1:3179464/1‑70 (MQ=255) aTACTTCCCGGTGTATGCGAACGACGGCAAACTGCTGCCGAACTTTATCTTCGTTGCCAACatcaaatcg > 1:374760/1‑70 (MQ=255) aCTTCCCGGTGTATGCGAACGACGGCAAACTGCTGCCGAACTTTAt > 1:1870719/1‑46 (MQ=255) aCTTCCCGGTGTATGCGAACGACGGCAAACTGCTGCCGAACTTTATCTTCGTTGCCaa > 1:1131393/1‑58 (MQ=255) cTTCCCGGTGTATGCGAACGACGGCAAACTGCTGCCGAACttt > 1:485402/1‑43 (MQ=255) cTTCCCGGTGTATGCGAACGACGGCAAACTGCTGCCGAACTTTATCTTCGTTg > 1:638392/1‑53 (MQ=255) cTTCCCGGTGTATGCGAACGACGGCAAACTGCTGCCGAACTTTATCTTCGTTGCCAACatc > 1:4598429/1‑61 (MQ=255) cTTCCCGGTGTATGCGAACGACGGCAAACTGCTGCCGAACTTTATCTTCGTTGCCAACATCGAATCGAAAg < 1:861804/71‑1 (MQ=255) ccGGTGTATGCGAACGACGGCAAACTGCTGCCGAACTTTATCTTCGTTGCCAACATCGAATCGAAAGATcc < 1:1333003/71‑1 (MQ=255) cGGTGTATGCGAACGACGGCAAACTGCTGCCGAACTTTATCTTCGTTGcc < 1:4154400/50‑1 (MQ=255) cGGTGTATGCGAACGACGGCAAACTGCTGCCGAACTTTATCTTCGTTGCCAACATCGAATCGAAAGATCCg < 1:3401079/71‑1 (MQ=255) tgtATGCGAGCGACGGCAAACTGCTGCCGAACTTTATc < 1:3037097/38‑1 (MQ=255) gtATGCGAACGACGGCAAACTGCTGCCGAACTTTATCTTCGTT‑CCAACATCGAATCGaaa > 1:4108387/1‑60 (MQ=255) tATGCGAACGACGGCAAACTGCTGCCGAACTTTATCTTCGTTGCCAACatcgaatcga < 1:1255470/58‑1 (MQ=255) tGCGAACGACGGCAAACTGCTGCCGAACTTTATCTTCGTTGCCAACatcgaa < 1:343488/52‑1 (MQ=255) tGCGAACGACGGCAAACTGCTGCCGAACTTTATCTTCGTTGCCAACATCGAATCGaaa > 1:299513/1‑58 (MQ=255) tGCGAACGACGGCAAACTGCTGCCGAACTTTATCTTCGTTGCCAACATCGAATCGAAAGATCCgcag > 1:957418/1‑67 (MQ=255) tGCGAACGACGGCAAACTGCTGCCGAACTTTATCTTCGTTGCCAACATCGAATCGAAAGATCCgcag > 1:2670966/1‑67 (MQ=255) tGCGAACGACGGCAAACTGCTGCCGAACTTTATCTTCGTTGCCAACATCGAATCGAAAGATCCgca > 1:4089226/1‑66 (MQ=255) gCGAACGACGGCAAACTGCTGCCGAACTTTATCTTCGTTGCCAACatcgaa > 1:2205509/1‑51 (MQ=255) cGAACGACGGCAAACTGCTGCCGATCTTTATCTTCGTTGCCAACATCGAATCGAAAGATCCGCAGCAGAtt < 1:2369436/71‑1 (MQ=255) cGAACGACGGCAAACTGCTGCCGAACTTTATCTTCGTTGCCAACATCGAATCGAAAGATcc > 1:1334039/1‑61 (MQ=255) cGAACGACGGCAAACTGCTGCCGAACTTTATCTTCGTTGCCAACATCGAATCGAAAGATCCGCAGCAGAtt < 1:3522389/71‑1 (MQ=255) cGAACGACGGCAAACTGCTGCCGAACTTTATCTTCGTTGCCAACATCGAATCGAAAGATCCGCAGCAGAtt < 1:2137570/71‑1 (MQ=255) cGAACGACGGCAAACTGCTGCCGAACTTTATCTTCGTTGCCAACATCGAATCGAAAGATCCGCAGCAGAt > 1:404096/1‑70 (MQ=255) aaCGACGGCAAACTGCTGCCGAACTTTATCTTCGTTGCCAACATCGAATCGAAAGATCCGCAGCAGATTAt < 1:853113/71‑1 (MQ=255) acgacgGCAAACTGCTGCCGAACTTTATCTTCGTTGCCAACATCGAATCGAAAGATCCGCAGCAGATTAtc > 1:3225766/1‑71 (MQ=255) | GGCTGAAGCGCTGGTTTACACCATGAAAGGTGACCAGAAATACTTCCCGGTGTATGCGAACGACGGCAAACTGCTGCCGAACTTTATCTTCGTTGCCAACATCGAATCGAAAGATCCGCAGCAGATTATC > W3110S.gb/3916838‑3916967 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |