| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,960,751 | C→T | 33.3% | R83H (CGT→CAT) | kdgK ← | ketodeoxygluconokinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,960,751 | 0 | C | T | 33.3% | 36.6 / 26.7 | 33 | R83H (CGT→CAT) | kdgK | ketodeoxygluconokinase |
| Reads supporting (aligned to +/- strand): ref base C (14/8); new base T (5/6); total (19/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.69e-01 | |||||||||||
CGTTCCGCCAGTAGTAGAACGTACGCTCGCCGGTGCTGTCGGTTTCAATGTAGTAAAGGCCCGGCAGACGGTTTTCCATCCGTTGGGTCAGGGAAGTATCAACGTTCTCGCCGTGCCAGGCGTCCAGCATCTGCTGG > W3110S.gb/3960683‑3960819 | cGTTCCGCCAGTAGTAGAACGTACGCTCGCCGGTGCTGTCGGTTTCAATGTAGTAAAGGCCCGGCAGACg < 1:3329535/70‑1 (MQ=255) ccAGTAGTAGAACGTACGCTCGCCGGTGCTGTCGGTTTCAATGTAGTAAAGGCCCGG‑‑GACGGTTTTCCATc > 1:993765/1‑71 (MQ=255) tagtagAACGTACGCTCGCCGGTGCTGTCGGTTTCAATGTAGTAAAGGCCCGGCAGACGGTTTTCCATCCg > 1:1282926/1‑71 (MQ=255) gtagAACGTACGCTCGCCGGTGCTGTCGGTTTCAATGTAGTAAAGGCCCGGCAGATGGTTTTCCATCCGtt < 1:581509/71‑1 (MQ=255) gtagAACGTACGCTCGCCGGTGCTGTCGGTTTCAATGTAGTAAAGGCCCGGCAGATGGTTTTCCATCCGtt > 1:2970232/1‑71 (MQ=255) tagAACGTACGCTCGCCGGTGCTGTCGGTTTCAATGTAGTAAAGGCCCGGCAGACGGTTTTCCATCCGTTg < 1:1329072/71‑1 (MQ=255) gAACGTACGCTCGCCGGTGCTGTCGGTTTCAATGTAGTAAAGGCCCGGCAGATGGTTTTCCATcc < 1:3708730/65‑1 (MQ=255) cTCGCCGGTGCTGTCGGTTTCAATGTAGTAAAGGCCCGGCAGACGGTTTTCCATCCGTTGGGTCAGGGAAg < 1:3494319/71‑1 (MQ=255) ccGGTGCTGTCGGTTTCAATGTAGTAAAGGCCCGGCAGACGGTTTTCCATCCGTTGGGTCAGGGAAGTATc < 1:734058/71‑1 (MQ=255) ggTGCTGTCGGTTTCAATGTAGTAAAGGCCCGGCAGATGGTTTTCCATCCGTTGGGTCAGGGAAGTATCaa > 1:1096964/1‑71 (MQ=255) gCTGTCGGTTTCAATGTAGTAAAGGCCCGGCAGACGGTTTTCCATCCGTTGGGTCAGGGAAg > 1:3329152/1‑62 (MQ=255) cTGTCGGTTTCAATGTAGTAAAGGCCCGGCAGATGGTTTTCCATCCGTTGGGTCAGGGAAGTATCAACGt < 1:1018481/70‑1 (MQ=255) cTGTCGGTTTCAATGTAGTAAAGGCCCGGCAGACGGTTTTCCATCCGTTGGGTCAGGGAAGTATCAACGtt < 1:4030783/71‑1 (MQ=255) ggTTTCAATGTAGTAAAGGCCCGGCAGATGGTTTTcc > 1:3785188/1‑37 (MQ=255) ggTTTCAATGTAGTAAAGGCCCGGCAGATGGTTTTCCATCCGTTGGGTCAgg > 1:498565/1‑52 (MQ=255) tttCAATGTAGTAAAGGCCCGGCAGACGGTTTTCCATCCGTTGGGTCAggg > 1:3910925/1‑51 (MQ=255) tCAATGTAGTAAAGGCCCGGCAGACGGTTTTCCATCCGTTGGGTCAGGGAAGTATCAACGTTCTCGCCGt > 1:4152162/1‑70 (MQ=255) tCAATGTAGTAAAGGCCCGGCAGACGGTTTTCCATCCGTTGGGTCAGGGAAGTATCAACGTTCTCGCCGTg > 1:498883/1‑71 (MQ=255) tCAATGTAGTAAAGGCCCGGCAGACGGTTTTCCATCCGTTGGGTCAGGGAAGTATCAACGTTCTCGCCGTg > 1:4146846/1‑71 (MQ=255) aaTGTAGTAAAGGCCCGGCAGATGGTTTTCCATCCGTTGGGTCa > 1:897007/1‑44 (MQ=255) tagtaAAGGCCCGGCAGACGGTTTTCCATCCGTTGGGTCAGGGaa < 1:2848816/45‑1 (MQ=255) taAATGCCCGGCAGACGGTTTTCCATCCGTTGGGTc < 1:2867318/36‑1 (MQ=255) taAAGGCCCGGCAGATGGTTTTCCATCCGTTGGGTCAcgg < 1:2381837/40‑1 (MQ=37) aaaGGCCCGGCAGACGGTTTTCCATCCGTTGGGTCAggg < 1:4153124/39‑1 (MQ=255) aaGGCCCGGCAGACGGTTTTCCATCCGTTGGGTCAGGGAAGTATCAACGTTCTCGCCGTGCCAGGCGTc > 1:1944577/1‑69 (MQ=255) gCCCGGCAGACGGTTTTCCATCCGTTGGGTCAGGGAAGTATCAACGTTCTCGCCGTGCCAGGCGTCCAGCa > 1:3776354/1‑71 (MQ=255) cGGCAGATGGTTTTCCATCCGTTGGGTCAGGGAAGt < 1:243649/36‑1 (MQ=255) cGGCAGATGGTTTTCCATCCGTTGGGTCAGGGAAGt < 1:249222/36‑1 (MQ=255) ggCAGACGGTTTTCCATCCGTTGGGTCAGGGAAGTATCAACGTTCTCGCCg > 1:1714166/1‑51 (MQ=255) gCAGACGGTTTTCCATCCGTTGGGTCAGGGAAGTATCAACGTTCTCGCCGTGc > 1:3627810/1‑53 (MQ=255) cAGACGGTTTTCCATCCGTTGGGTCAGGGAAGTATCAACGTTCTCGCCGTGCCAGGCGTCCAGCATctgct > 1:3488060/1‑71 (MQ=255) aGACGGTTTTCCATCCGTTGGGTCAGGGAAGTATCAACGTTCTCGCCGTGCCAGGCGTCCAGCATctgctg > 1:3659152/1‑71 (MQ=255) gACGGTTTTCCATCCGTTGGGTCAGGGAAGTATCAACGTTCTCGCCGTGCCAGGCGTCCAGCATCTGCTgg > 1:798804/1‑71 (MQ=255) | CGTTCCGCCAGTAGTAGAACGTACGCTCGCCGGTGCTGTCGGTTTCAATGTAGTAAAGGCCCGGCAGACGGTTTTCCATCCGTTGGGTCAGGGAAGTATCAACGTTCTCGCCGTGCCAGGCGTCCAGCATCTGCTGG > W3110S.gb/3960683‑3960819 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |