| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,000,806 | A→T | 10.8% | D37V (GAT→GTT) | yhiO → | predicted universal stress (ethanol tolerance) protein B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,000,806 | 0 | A | T | 10.8% | 193.3 / 15.3 | 74 | D37V (GAT→GTT) | yhiO | predicted universal stress (ethanol tolerance) protein B |
| Reads supporting (aligned to +/- strand): ref base A (29/37); new base T (3/5); total (32/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01 | |||||||||||
GTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGCCAACCCAACAAACAG > W3110S.gb/4000736‑4000873 | gTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGa > 1:1189894/1‑71 (MQ=255) tGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATc < 1:2629820/70‑1 (MQ=255) gCATTGTTAACATGGCACGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATcc < 1:2962847/70‑1 (MQ=255) cATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCa < 1:2583235/70‑1 (MQ=255) aTTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCAtt > 1:3457680/1‑71 (MQ=255) tGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGc > 1:1533269/1‑71 (MQ=255) gTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTg > 1:185037/1‑69 (MQ=255) gTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGct > 1:3431963/1‑71 (MQ=255) tAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGctc > 1:2155492/1‑70 (MQ=255) cATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATc < 1:3674873/71‑1 (MQ=255) aTGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCa < 1:1588999/71‑1 (MQ=255) tGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGTTCCATTGCTCTATCaa < 1:538591/71‑1 (MQ=255) tGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGTTCCATTGCTCTATCaa < 1:475929/71‑1 (MQ=255) tGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCaa < 1:2817700/71‑1 (MQ=255) ggCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATc < 1:1712035/56‑1 (MQ=255) gcgcgcTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAata > 1:133810/1‑71 (MQ=255) gcgcTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATg > 1:3550778/1‑71 (MQ=255) gcTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGtt < 1:900039/71‑1 (MQ=255) gcTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGtt < 1:2280927/71‑1 (MQ=255) tATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATc < 1:916614/61‑1 (MQ=255) aTTTCTCATCACTACGCTCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGtt < 1:2711565/68‑1 (MQ=255) tttCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGTTCCATTGCTCTATCAATATGTTGa < 1:4427742/69‑1 (MQ=255) tttCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATc < 1:2152787/47‑1 (MQ=255) tttCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATc < 1:2323103/47‑1 (MQ=255) tttCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCa > 1:2843102/1‑49 (MQ=255) tttCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGtt < 1:3538932/67‑1 (MQ=255) tctcATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATc < 1:4433324/45‑1 (MQ=255) tctcATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCa > 1:1647834/1‑58 (MQ=255) tctcATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATgg < 1:2663065/70‑1 (MQ=255) ctcATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGTTCCa > 1:3622941/1‑46 (MQ=255) ctcATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATc < 1:3507689/44‑1 (MQ=255) tcatcaCTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAgg > 1:4019583/1‑71 (MQ=255) catcaCTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAggg > 1:3970964/1‑71 (MQ=255) catcaCTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAggg > 1:564290/1‑71 (MQ=255) tcaCTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGTTCCATTGCTCTATCAATATGtt < 1:1780861/60‑1 (MQ=255) caCTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGctct > 1:2636433/1‑48 (MQ=255) caCTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGctct > 1:1332314/1‑48 (MQ=255) caCTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAata > 1:2232111/1‑55 (MQ=255) cgcgcgTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCttttt < 1:503379/71‑1 (MQ=255) gcgcgTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCtcaatcaa < 1:3011908/47‑1 (MQ=255) ttgttAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCa < 1:171562/41‑1 (MQ=255) ttgttAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTg < 1:4260379/49‑1 (MQ=255) ttgttAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGAt < 1:4637766/51‑1 (MQ=255) ttgttAGTGGTACTGCCTAACTGCGATCCATTGCTCTATCAATATGTTg < 1:2173471/49‑1 (MQ=255) gttAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCaa > 1:652448/1‑40 (MQ=255) gttAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTca < 1:1436564/70‑1 (MQ=255) gttAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTca < 1:1100356/70‑1 (MQ=255) ttAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTcaca > 1:3808437/1‑71 (MQ=255) ttAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTcaca < 1:3865952/71‑1 (MQ=255) tAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACAt > 1:911484/1‑71 (MQ=255) aGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATg > 1:783584/1‑71 (MQ=255) tGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGtt < 1:630274/41‑1 (MQ=255) tGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCtttt < 1:870470/57‑1 (MQ=255) gTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGcc > 1:3571853/1‑70 (MQ=255) tACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATg < 1:69300/42‑1 (MQ=255) tACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATg < 1:2842267/42‑1 (MQ=255) tACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGa < 1:3493085/44‑1 (MQ=255) aCTGCGTAACTGCGTTCCATTGCTCTATCAATATGTTGATg < 1:3203083/41‑1 (MQ=255) tGCGTAACTGCGATCCATTGCTCTATCAATATGtt < 1:2044299/35‑1 (MQ=255) gcttAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGccaaccc > 1:3794236/4‑70 (MQ=255) gCGTAACTGCGTTCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACAt > 1:4255135/1‑61 (MQ=255) gCGTAACTGCGTTCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACAt > 1:2556298/1‑61 (MQ=255) gCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTa > 1:3074848/1‑53 (MQ=255) gCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTcaca > 1:3788907/1‑60 (MQ=255) gCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGccaaccc > 1:1790591/1‑70 (MQ=255) tAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGCCAACCcaaca < 1:1636055/71‑1 (MQ=255) tAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGCCAACCcaaca < 1:2361224/71‑1 (MQ=255) aCTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGCCAACCCaacaaa < 1:2147253/71‑1 (MQ=255) aCTGCGATCCATTGCTCTATCAATATGTTGATGGA‑GGGGCTTTTTTACCTCACATGGCCAACCCaacaaa < 1:1596514/70‑1 (MQ=255) tGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTca < 1:3693174/51‑1 (MQ=255) gCGATCCATTGTTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGCCAACCCAACAAACAg > 1:1504994/1‑71 (MQ=255) gCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACAt > 1:1769385/1‑53 (MQ=255) gCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATgg > 1:1522919/1‑55 (MQ=255) gCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGCCAACCCAACAAACAg > 1:2364612/1‑71 (MQ=255) cGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGCCAACCCAACAAACAg > 1:2344378/1‑70 (MQ=255) aTCCATTGCTCTATCAATATGTTGATGGAGGGGGCtttttt < 1:3335448/41‑1 (MQ=255) | GTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGCCAACCCAACAAACAG > W3110S.gb/4000736‑4000873 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |