| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,068,392 | T→C | 29.5% | M493T (ATG→ACG) | glgB → | 1,4‑alpha‑glucan branching enzyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,068,392 | 0 | T | C | 29.5% | 83.3 / 48.9 | 61 | M493T (ATG→ACG) | glgB | 1,4‑alpha‑glucan branching enzyme |
| Reads supporting (aligned to +/- strand): ref base T (20/23); new base C (14/4); total (34/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.63e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01 | |||||||||||
ATATGGGCGGTCTGGGCTTCTGGTACAAGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATTCTCTACAACTACACT > W3110S.gb/4068326‑4068462 | atatGGGCGGTCTGGGCTTCTGGTACAAGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAg > 1:3817594/1‑71 (MQ=255) tatGGGCGGTCTGGGCTTCTGGTACAAGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGc > 1:4551253/1‑71 (MQ=255) tGGGCGGTCTGGGCTTCTGGTACAAGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTc > 1:2386830/1‑71 (MQ=255) ggCGGTCTGGGCTTCTGGTACAAGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCg < 1:2061853/70‑1 (MQ=255) ggCGGTCTGGGCTTCTGGTACAAGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGa < 1:1497472/71‑1 (MQ=255) ggCGGTCTGGGCTTCTGGTACAAGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGa > 1:4306869/1‑71 (MQ=255) tGGGCTTCTGGTACAAGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGtt > 1:4037359/1‑71 (MQ=255) tGGGCTTCTGGTACAAGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACACGAAGCTCGACCCGGtt > 1:3105439/1‑71 (MQ=255) ggCTTCTGGTACAAGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGtt < 1:3410588/69‑1 (MQ=255) gTACAAGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACAt < 1:4437700/45‑1 (MQ=255) gTACAAGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTc < 1:1747625/68‑1 (MQ=255) tACAAGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACACGAAGCTCGACCCGGTTTATCGTCAGTa < 1:4039927/71‑1 (MQ=255) aCAAGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTat > 1:53535/1‑71 (MQ=255) cAAGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTc > 1:3480512/1‑49 (MQ=255) cAAGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTat < 1:3551329/70‑1 (MQ=255) aaGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTatca < 1:2622649/71‑1 (MQ=255) tGGAACCTCGGCTCGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTatcat < 1:3847596/69‑1 (MQ=255) aCCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAg > 1:2807679/1‑59 (MQ=255) ccTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCgg < 1:1227199/47‑1 (MQ=255) ccTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATa < 1:113702/71‑1 (MQ=255) cTCGGCTGGATGCATGACCCCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATaa < 1:966385/71‑1 (MQ=255) cTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTAtcatca > 1:3639782/1‑65 (MQ=255) cTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATa < 1:2183576/70‑1 (MQ=255) cTCGGCTGGATGCATGACACCCTGGACTACACGAAGCTCGACCCGGTTTATCGTCAg > 1:2040620/1‑57 (MQ=255) tCGGCTGGATGCATGACACCCTGGACTACACGAAGCTCGa > 1:1909167/1‑40 (MQ=255) cGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATaaa > 1:4574149/1‑70 (MQ=255) cGGCTGGATGCATGACACCCTGGACTACACGAAGCTCGACCCGGTTTATCGTCAg > 1:1514770/1‑55 (MQ=255) ggCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATa < 1:498406/67‑1 (MQ=255) ggCTGGATGCATGACACCCTGGACTACACGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACt < 1:273629/71‑1 (MQ=255) ggCTGGATGCATGACACCCTGGACTACACGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACt < 1:1745633/71‑1 (MQ=255) gCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATaaa < 1:3973299/68‑1 (MQ=255) gCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTg < 1:4191868/71‑1 (MQ=255) ggATGCATGACACCCTGGACTACACGAAGCTCGACCCGGtt > 1:3440413/1‑41 (MQ=255) gATGCATGACACCCTGGACTACACGAAGCTCGACCCGGTTTATCGTc < 1:2052514/47‑1 (MQ=255) tGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTAt > 1:657947/1‑41 (MQ=255) cATGACACCCTGGACTACACGAAGCTCGACCCGGTTTATCGTCAGTat > 1:2429992/1‑48 (MQ=255) cATGACACCCTGGACTACACGAAGCTCGACCCGGTTTATCGTCAGTat > 1:1415617/1‑48 (MQ=255) cATGACACCCTGGACTACACGAAGCTCGACCCGGTTTATCGTCAGTat > 1:1519456/1‑48 (MQ=255) aTGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCgg < 1:2705180/70‑1 (MQ=255) tGACTCCCTGGACTACATGAAGCTCGACCCTGTTTATCGTCAGTatc < 1:1147207/47‑1 (MQ=39) tGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTc < 1:3304213/41‑1 (MQ=255) tGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGa < 1:1371841/54‑1 (MQ=255) tGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGa < 1:2869008/54‑1 (MQ=255) acacCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCgg > 1:2315396/1‑67 (MQ=255) acacCCTGGACTACATGAAGCTAGACCCGGTTTATCGTCAg > 1:1794392/1‑41 (MQ=255) acacCCTGGACTACACGAAGCTCGACCCGGTTTATCGTCAGTa > 1:3079013/1‑43 (MQ=255) acacCCTGGACTACACGAAGCTCGACCCGGTTTATCGTCAGTa > 1:530513/1‑43 (MQ=255) acacCCTGGACTACACGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATaaa > 1:2084887/1‑56 (MQ=255) cacCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGAc > 1:3061619/1‑60 (MQ=255) tGGACTACATGAAGCTCGACCCGGGTTATCGTCAGTatca > 1:2169104/1‑40 (MQ=255) ggACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGAt < 1:958433/63‑1 (MQ=255) ggACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATTCTCTAc > 1:702432/1‑70 (MQ=255) ggACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGAACTTCGGGa > 1:46425/1‑62 (MQ=255) ggACTACACGAAGCTCGACCCGGTTTATCGTCAGTa > 1:1410595/1‑36 (MQ=255) ggACTACACGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATTCTCTa > 1:3636316/1‑69 (MQ=255) gACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATTCTCTAc < 1:4492034/69‑1 (MQ=255) gACTACATGAAGCTCGACCAGGTTTATCGTCAGTATCATCACGATaaa > 1:3001212/1‑48 (MQ=255) gACTACACGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGAATCTCTACaa > 1:3820898/1‑71 (MQ=255) tACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCAc < 1:4266853/39‑1 (MQ=255) cATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATTCTCTACAACTaca < 1:2715395/71‑1 (MQ=255) aTGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATTCTCTa > 1:1446140/1‑62 (MQ=255) aTGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATTCTCTACAACTacac > 1:1896501/1‑71 (MQ=255) tGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATTCTCTACAACTACACt > 1:1793205/1‑71 (MQ=255) | ATATGGGCGGTCTGGGCTTCTGGTACAAGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATTCTCTACAACTACACT > W3110S.gb/4068326‑4068462 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |