| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,085,470 | G→A | 21.0% | R622C (CGT→TGT) | malT ← | DNA‑binding transcriptional activator, maltotriose‑ATP‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,085,470 | 0 | G | A | 21.0% | 77.4 / 16.1 | 38 | R622C (CGT→TGT) | malT | DNA‑binding transcriptional activator, maltotriose‑ATP‑binding |
| Reads supporting (aligned to +/- strand): ref base G (14/16); new base A (5/3); total (19/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01 | |||||||||||
TTGCCATTCCCCAGCAGGTTTTCCAGACGGTTCAGCTGGCTACGGGCGTTATCTAAATCACCACGGGCCAGCGAGCATTGAATCAACATTGCCAGGCACTGAAGCTGTTGCTGTGGCTGATAAGACGA > W3110S.gb/4085406‑4085533 | ttGCCATTCCCCAGCAGGTTTTCCAGACGGTTCAGCTGGCTACGGGCGTTATCTAAATCACCACGGGCCAg > 1:420417/1‑71 (MQ=255)ttGCCATTCCCCAGCAGGTTTTCCAGACGGTTCAGCTGGCTACGGGCGTTATCTAAATCACCACGGGCCAg > 1:1901708/1‑71 (MQ=255) tGCCATTCCCCAGCAGGTTTTCCAGACGGTTCAGCTGGCTACGGGCGTTATCTAAATCACCACGGGCCAGc > 1:1649531/1‑71 (MQ=255) ccccAGCAGGTTTTCCAGACGGTTCAGCTGGCTACGGGCGTTATCTAAATCACCACGGGCCAGCGAGCAtt > 1:741255/1‑71 (MQ=255) cagcagGTTTTCCAGACGGTTCAGCTGGCTACGGGCGTTATCTAAATCACCACAGGCCAGCGAGCATTGaa > 1:2524538/1‑71 (MQ=255) agGTTTTCCAGACGGTTCAGCTGGCTACGGGCGTTATCTAAATCACCACGGGCCAGCGAGCATTGAATCaa < 1:678942/71‑1 (MQ=255) tttCCAGACGGTTCAGCTGGCTACGGGCGTTATCTAAATCACCACGGGCCAGCGAGCATTGAATCAACAtt > 1:2920596/1‑71 (MQ=255) ttCCAGACGGTTCAGCTGGCTACGGGCGTTATCTAAATCACCACGGGCCAGCGAGCATTGAATCAACAtt < 1:289151/70‑1 (MQ=255) aGACGGTTCAGCTGGCTACGGGCGTTATCTAAATCACCACAGGCCAGCGAGCATTGAATCAACATTg < 1:292329/67‑1 (MQ=255) gACGGTTCAGCTGGCTACGGGCGTTATCTAAATCACCACGGGCCAGCGAGCATTGAATCAACATTGCCAgg < 1:2529391/71‑1 (MQ=255) cGGTTCAGCTGGCTACGGGCGTTATCTAAATCACCACGGGCCAGCGAGCATTGAATCAACATTGCCAGGCa > 1:4044584/1‑71 (MQ=255) ggTTCAGCTGGCTACGGGCGTTATCTAAATCACCACAGGCCAGCGAGCATTGAATCAACATTGCCAGGCAc > 1:4354946/1‑71 (MQ=255) ttCAGCTGGCTACGGGCGTTATCTAAATCACCACGGGCCAGCGAGCATTGAATCAACATTGCCAGGCACt > 1:351499/1‑70 (MQ=255) gCTACGGGCGTTATCTAAATCACCACGGGCCAGCGAGGATTGAATCAACATTG‑CAGGCACTGAAGc > 1:4147662/1‑66 (MQ=255) gCTACGGGCGTTATCTAAATCACCACGGGCCAGCGAGCATTGAATCAACATTGCCAGGCACTGAAGCTGtt < 1:3941653/71‑1 (MQ=255) ttATCTAAATCACCACAGGCCAGCGAGCATTGAATCAACATTGCCAGGCACTGAAGCTGTTGCtgtg < 1:3622267/67‑1 (MQ=255) aTCTAAATCATCACGGGCCAGCGAGCATTGAATCAACATTGc > 1:1176686/1‑42 (MQ=255) aTCTAAATCACCACGGGCCAGCGAGCATTGAATCAACATTGCCAGGCACTGAAGCTGTTGCTGTGGCTGa < 1:2972855/70‑1 (MQ=255) aTCTAAATCACCACGGGCCAGCGAGCATTGAATCAACATTGCCAGGCACTGAAGCTGTTGCTGTGGCTGa < 1:647416/70‑1 (MQ=255) aTCTAAATCACCACGGGCCAGCGAGCATTGAATCAACATTGCCAGGCACTGAAGCTGTTGCTGTGGCTGa > 1:3889072/1‑70 (MQ=255) aTCTAAATCACCACGGGCCAGCGAGCATTGAATCAACATTGCCAGGCACTGAAGCTGTTGCTGTGGCTGa < 1:79527/70‑1 (MQ=255) aTCTAAATCACCACGGGCCAGCGAGCATTGAATCAACATTGCCAGGCACTGAAGCTGTTGCTGTGGCTGa > 1:1465571/1‑70 (MQ=255) tCTAAATCACCACGGGCCAGCGAGCATTGAATCAACATTGc < 1:1722705/41‑1 (MQ=255) cTAAATCACCACGGGCCAGCGAGCATTGAATCAACATTGCCAGGCACTGAAGCt < 1:594645/54‑1 (MQ=255) cTAAATCACCACAGGCCAGCGAGCATTGAATCAACATTGCCAGGCACTGAAGCTGt > 1:857497/1‑56 (MQ=255) tAAATCACCACGGGCCAGCGAGCATTGAATCAACATTGCCAGGCACTGAAGCTGTTGc < 1:4393412/58‑1 (MQ=255) aaaTCACCACGGGCCAGCGAGCATTGAATCAACATTGCCAGGCa > 1:505305/1‑44 (MQ=255) aaTCACCACGGGCCAGCGAGCATTGAATCAACAtt < 1:3997016/35‑1 (MQ=255) aaTCACCACGGGCCAGCGAGCATTGAATCAACAtt < 1:1223132/35‑1 (MQ=255) aaTCACCACGGGCCAGCGAGCATTGAATCAACATTGCCAgg < 1:835274/41‑1 (MQ=255) aaTCACCACGGGCCAGCGAGCATTGAATCAACATTGCCAGGCACTGAAGCAGTTGCTGTGGCTGATAAgac > 1:3340992/1‑71 (MQ=255) aTCACCACGGGCCAGCGAGCATTGAATCAACATTGCCAgg < 1:4261905/40‑1 (MQ=255) tCACCACAGGCCAGCGAGCATTGAATCAACATTGCCAGGCACTGAAGCTGTTGc > 1:1542658/1‑54 (MQ=255) tCACCACAGGCCAGCGAGCATTGAATCAACATTGCCAGGCACTGAAGCTGTTGCTGTGGCTGATAAgacga > 1:1722932/1‑71 (MQ=255) accacAGGCCAGCGAGCATTGAATCAACATTGCCAGGCACTGAAGCTGTTGCtgt < 1:2261374/55‑1 (MQ=255) cacGGGCCAGCGAGCATTGAATCAACATTGCCAGGCACTGAAGCTGTTGc < 1:3823194/50‑1 (MQ=255) cacGGGCCAGCGAGCATTGAATCAACATTGCCAGGCACTGAAGCTGTTGc < 1:2708330/50‑1 (MQ=255) acGGGCCAGCGAGCATTGAATCAACATTGCCAGGCACTGAAGCTGTTGCTGTGGCTGATAAga > 1:2855042/1‑63 (MQ=255) | TTGCCATTCCCCAGCAGGTTTTCCAGACGGTTCAGCTGGCTACGGGCGTTATCTAAATCACCACGGGCCAGCGAGCATTGAATCAACATTGCCAGGCACTGAAGCTGTTGCTGTGGCTGATAAGACGA > W3110S.gb/4085406‑4085533 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |