| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,092,715 | G→A | 16.6% | A382V (GCG→GTG) | gntT ← | gluconate transporter, high‑affinity GNT I system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,092,715 | 0 | G | A | 16.6% | 116.9 / 14.5 | 48 | A382V (GCG→GTG) | gntT | gluconate transporter, high‑affinity GNT I system |
| Reads supporting (aligned to +/- strand): ref base G (25/15); new base A (5/3); total (30/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TACTCTTTGAACAGCCAGAAGCCCGGATCGTTCACATGAGAGAAAATCACACTACCGGAACCAACCGCAATAACCATCAGCTCCGGGCTAACACCCGTCGTTGCAATCAGCGGTGCCGCGATACCACCCGCAGT > W3110S.gb/4092649‑4092782 | tACTCTTTGAACAGCCAGAAGCCCGGATCGTTCACATGAGAGAAAATCACACTACCGGAACCAACCGCAAt < 1:2450307/71‑1 (MQ=255) ctTTGAACAGCCAGAAGCCCGGATCGTTCACATGAGAGAAAATCACACTACCGGAACCAACCGCAATAAc > 1:4598899/1‑70 (MQ=255) aaGCCCGGATCGTTCACATGAGAGAAAATCACACTACCGGAACCAACCGCAATAACCATCAGCTCCGGGCt < 1:83543/71‑1 (MQ=255) aaGCCCGGATCGTTCACATGACAGAAAATCACACTACCGGAACCAACCGCAATAACCATCAGCCCCGGGCt < 1:480409/71‑1 (MQ=255) aGCCCGGAGCGTTCACATGAGAGAAAATCACACTACCGGAACCAACCGCAATAACCATCAGCTCCGGGCt < 1:4381131/70‑1 (MQ=255) gCCCGGATCGTTCACATGAGAGAAAATCACACTACCGGAACCAACCGCAATAACCATc > 1:3403290/1‑58 (MQ=255) gCCCGGATCGTTCACATGAGAGAAAATCACACTACCGGAACCAACCGCAATAACCATCAGCTCCGGGCTaa > 1:772743/1‑71 (MQ=255) cccGGATCGTTCACATGAGAGAAAATCACACTACCGGAACCAACCGCAATAACCATCAGCTCCGGGCTaa > 1:1570221/1‑70 (MQ=255) gATCGTTCACATGAGAGAAAATCACACTACCGGAACCAACCGCAATAACCATCAGCTCCGGGCTAACAccc < 1:4446897/71‑1 (MQ=255) aTCGTTCACATGAGAGAAAATCACACTACCGGAACCAACCGCAATAACCATCAGCTCCGGGCTAACACCcg > 1:1515624/1‑71 (MQ=255) aTCGTTCACATGAGAGAAAATCACACTACCGGAACCAACCACAATAACCATCAGCTCCGGGCt < 1:1019472/63‑1 (MQ=255) tCACATGAGAGAAAATCACACTACCGGAACCAACCGCAATAAc > 1:2842269/1‑43 (MQ=255) tCACATGAGAGAAAATCACACTACCGGAACCAACCGCAATAACCATCAGCTCCGGGCTAACACCcttc < 1:1771735/68‑1 (MQ=255) cacaTGAGAGAAAATCACACTACCGGAACCAACCGCAATa > 1:4505048/1‑40 (MQ=255) cacaTGAGAGAAAATCACACTACCGGAACCAACCGCAATAACCATCAGCTCCGGGCTAac > 1:1489922/1‑60 (MQ=255) cacaTGAGAGAAAATCACACTACCGGAACCAACCACAATAACCATCAGCTCCGGGCTAACACCCGTCGTTg > 1:2454837/1‑71 (MQ=255) cacaTGAGAGAAAATCACACTACCGGAAACAACCGCAATAACCATCAGCTCCGGGCTAac > 1:4120384/1‑60 (MQ=255) acaTGAGAGAAAATCACACTACCGGAACCAACCACAATAACCATCAGCTCCGGGCTAACACCCGTCGTTGc < 1:4516385/71‑1 (MQ=255) caTGAGAGAAAATCACACTACCGGAAGCAACCGCAATAACCATCAGCTCCGGGCTAACACCCGTCGTTGc > 1:2351180/1‑70 (MQ=255) aTGAGAGAAAATCACACTACCGGAACCAACCGCAATAACCATCAGCTCCGGGCTAACACCCGTCGTTGCaa < 1:1670272/71‑1 (MQ=255) tGAGAGAAAATCACACTACCGGAACCAACCGCAATAACCATCAGCTCCg < 1:3105036/49‑1 (MQ=255) tGAGAGAAAATCACACTACCGGAACCAACCACAATAACCATCAGCTCCGGGCTAACACCCGTCGTTGCAAt > 1:1733542/1‑71 (MQ=255) agagaAAATCACACTACCGGAACCAACCGCAATaa > 1:3245123/1‑35 (MQ=255) agagaAAATCACACTACCGGAACCAACCGCAATAACCATCAGCTCCGGGCTAACACCCGTCGTTGCAAt > 1:185341/1‑69 (MQ=255) agaAAATCACACTACCGGAACCAACCGCAATAACCATCa > 1:4640102/1‑39 (MQ=255) agaAAATCACACTACCGGAACCAACCGCAATAACCATCAGCTCCGGGCTAACACCCGTCGTTGCAATCAGc > 1:4641203/1‑71 (MQ=255) gaAAATCACACTACCGGAACCAACCGCAATAACCATCAGCTCCGGGCTAACACCCGTCGTTGCAATCAGCg > 1:2396068/1‑71 (MQ=255) gaAAATCACACTACCGGAACCAACCGCAATAACCATCAGCTCCGGGCTAACACCCGTCGTTGCAATCAGCg > 1:3739905/1‑71 (MQ=255) aaaaTCACACTACCGGAACCAACCGCAATAACCATCAGCTCCGGGCTAACACCCGTCGTTGc < 1:4113524/62‑1 (MQ=255) aaaaTCACACTACCGGAACCAACCGCAATAACCATCAGCTCCGGGCTAACACCCGTCGTTGCAATCAGCg > 1:2190235/1‑70 (MQ=255) aaaaTCACACTACCGGAACCAACCACAATAACCAt < 1:1780258/35‑1 (MQ=255) aaaTCACACTACCGGAACCAACCGCAATAACCATCAGCTCCGGGCTAACACCCGTCGTTGCAATCAGCGGt < 1:3928222/71‑1 (MQ=255) aaaTCACACTACCGGAACCAACCGCAATAACCATCAGCTCCGGGCTAACACCCGTCGTTGCAATCAGCGGt > 1:2039534/1‑71 (MQ=255) aaTCACACTACCGGAACCAACCGCAATAACCATCAGCTCCg > 1:3577995/1‑41 (MQ=255) aaTCACACTACCGGAACCAACCGCAATAACCATCAGCTCCGGGCTAACACCCGTCGTTGCAATCAGCGGTg > 1:1399349/1‑71 (MQ=255) acacTACCGGAACCAACCGCAATAACCATCAGCTCCGGGCTAACACCCGTCGTTGCag > 1:4140010/1‑57 (MQ=255) acacTACCGGAACCAACCGCAATAACCATCAGCTCCGGGCTAACACCCGTCGTTGCaa > 1:1104440/1‑58 (MQ=255) acacTACCGGAACCAACCGCAATAACCATCAGCTCCGGGCTAACACCCGTCGTTGCAATCAGCGGTGCcgc < 1:2718698/71‑1 (MQ=255) acacTACCGGAACCAACCACAATAACCATCAGCTCCGGGCTAACACCcgtcg > 1:3947101/1‑52 (MQ=255) acTACCGGAACCAACCGCAATAACCATCAGCTCCGGGCTAACACCCGTCGTTGCAATCAGCGGTGCCGCGa < 1:748446/71‑1 (MQ=255) tACCGGAACCAACCGCAATAACCATCAGCTCCGGGCt < 1:1297375/37‑1 (MQ=255) tACCGGAACCAACCGCAATAACCATCAGCTCCGGGCTAACACCCGTCGTTGCAATCAGCGGTGCCGCGa > 1:3888551/1‑69 (MQ=255) tACCGGAACCAACCGCAATAACCATCAGCTCCGGGCTAACACCCGTCGTTGCAATCAGCGGTGCCGCGa < 1:2995240/69‑1 (MQ=255) ccGGAACCAACCGCAATAACCATCAGCTCCGGGCTaa > 1:1540753/1‑37 (MQ=255) ggAACCAACCACAATAACCATCAGCTCCGGGCTAACACCCGTCGTTGCAATCAGCGGTGCCGCGATaccac > 1:4256758/1‑71 (MQ=255) ggAACCAACCACAATAACCATCAGCTCCGGGCTAACACCCGTCGTTGCAATCAGCGGTGCCGCGATaccac > 1:2761996/1‑71 (MQ=255) caaccGCAATAACCATCAGCTCCGGGCTAACAccc < 1:2664125/35‑1 (MQ=255) accGCAATAACCATCAGCTCCGGGCTAACACCCGTCGTTGCAATCAGCGGTGCCGCGATACCACCCGCAGt > 1:3746117/1‑71 (MQ=255) | TACTCTTTGAACAGCCAGAAGCCCGGATCGTTCACATGAGAGAAAATCACACTACCGGAACCAACCGCAATAACCATCAGCTCCGGGCTAACACCCGTCGTTGCAATCAGCGGTGCCGCGATACCACCCGCAGT > W3110S.gb/4092649‑4092782 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |