| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,125,813 | C→T | 22.4% | P152L (CCT→CTT) | rpe → | D‑ribulose‑5‑phosphate 3‑epimerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,125,813 | 0 | C | T | 22.4% | 136.5 / 35.3 | 67 | P152L (CCT→CTT) | rpe | D‑ribulose‑5‑phosphate 3‑epimerase |
| Reads supporting (aligned to +/- strand): ref base C (28/24); new base T (8/7); total (36/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01 | |||||||||||
TGATGGATAAGCTGGATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAGA > W3110S.gb/4125744‑4125882 | tGATGGATAAGCTGGATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCt > 1:2164892/1‑71 (MQ=255) aTGGATAAGCTGGATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTc < 1:2047813/70‑1 (MQ=255) aTGGATAAGCTGGATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCa < 1:469203/71‑1 (MQ=255) aTGGATAAGCTGGATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCa < 1:4588347/71‑1 (MQ=255) tGGATAAGCTGGATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCa > 1:1740291/1‑70 (MQ=255) ggATAAGCTGGATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCaaa > 1:3455987/1‑71 (MQ=255) gATAAGCTGGATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCaaa < 1:4439088/70‑1 (MQ=255) aTAAGCTGGATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAaca > 1:2565559/1‑71 (MQ=255) aTAAGCTGGATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAaca < 1:3496162/71‑1 (MQ=255) tGGATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGa > 1:3448811/1‑70 (MQ=255) ggATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGAt > 1:945471/1‑70 (MQ=255) aTCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCTTCAAACACTGGATa < 1:736268/64‑1 (MQ=255) aTCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCTTCAAACACTGGATAAACTgcg > 1:1407248/1‑71 (MQ=255) ccTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTgcgc > 1:124484/1‑70 (MQ=255) ctgctgATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGcgcg < 1:3853965/70‑1 (MQ=255) gctgATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAg > 1:3288731/1‑71 (MQ=255) aTGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCTTCAAACACTGGATAAACTGCGCGAAGTAc < 1:825688/70‑1 (MQ=255) tGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCaaa < 1:3837104/45‑1 (MQ=255) ccGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCTTCa < 1:1317778/40‑1 (MQ=255) gTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCTTCaaa > 1:1159043/1‑40 (MQ=255) tCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCTTCAAACACTGGATAAAc < 1:264121/51‑1 (MQ=255) tCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCTTCAAACACTGGATAAAc < 1:1471552/51‑1 (MQ=255) tCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAg > 1:3625114/1‑60 (MQ=255) tCAACCCTGGTTTCGGCGGTCAGTCTTTCATTACTCAAACACTGGATAAACTGcgcg < 1:1716061/57‑1 (MQ=255) cAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTa > 1:1621784/1‑71 (MQ=255) aaCCCTGGTTTCGGCGGTCAGTCTTTCATTCTTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTAt > 1:2203428/1‑71 (MQ=255) aaCCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTa > 1:3305553/1‑70 (MQ=255) cccTGGTTTCGGCTGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGcgcg < 1:999435/53‑1 (MQ=255) ccTGGTTTCGTCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATcga > 1:344909/1‑71 (MQ=255) cTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATcgac < 1:3560148/71‑1 (MQ=255) tGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTa > 1:2901659/1‑55 (MQ=255) tGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATcgac > 1:3051251/1‑70 (MQ=255) tGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATcgac > 1:469905/1‑70 (MQ=255) ggTTTCGGCGGTCAGTCTTTCATTCTTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATcgac > 1:3565810/1‑69 (MQ=255) gTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTg > 1:725884/1‑44 (MQ=255) ttCGGCGGTCAGTCTTTCATTCTTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTc > 1:3516190/1‑71 (MQ=255) ttCGGCGGTCAGTCTTTCATTCTTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTc > 1:1877696/1‑71 (MQ=255) tCGGCGGTCAGTCTTTCATTCTTCAAACACTGGATAAAc > 1:2624732/1‑39 (MQ=255) tCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCt > 1:898616/1‑71 (MQ=255) gcggTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAg < 1:3442118/45‑1 (MQ=255) gTCAGTCTTTCATTCCTCAAACACTGGATAAACTGcgcg < 1:2972847/39‑1 (MQ=255) gTCAGTCTTTCATTCCTCAAACACTGGATAAACTGcgcg < 1:2707590/39‑1 (MQ=255) gTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATcga > 1:1174775/1‑58 (MQ=255) gTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCttt < 1:2856406/71‑1 (MQ=255) gTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCttt < 1:3908355/71‑1 (MQ=255) tCAGTCTTTCATTCCTCAAACACTGGATAAACTGcgcg < 1:519976/38‑1 (MQ=255) tCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTg < 1:3971204/71‑1 (MQ=255) cAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGt > 1:2990005/1‑45 (MQ=255) cAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGa > 1:768063/1‑71 (MQ=255) aGTCTTTCATTCTTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGa > 1:693349/1‑70 (MQ=255) aGTCTTTCATTCTTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGAc < 1:345005/71‑1 (MQ=255) aGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTAc < 1:1648539/42‑1 (MQ=255) aGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGa > 1:2416528/1‑70 (MQ=255) aGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGa < 1:4350823/70‑1 (MQ=255) gTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAg < 1:4060074/38‑1 (MQ=255) gTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACa < 1:2769719/71‑1 (MQ=255) tttCATTCTTCAAACACTGGATAAACTGCGCGAAGTAc < 1:3422406/38‑1 (MQ=255) tttCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATcga > 1:3544888/1‑51 (MQ=255) tttCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTc > 1:2416914/1‑71 (MQ=255) tttCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTc > 1:1141756/1‑71 (MQ=255) tttCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTc > 1:1555702/1‑71 (MQ=255) tCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTAt > 1:4629609/1‑46 (MQ=255) tCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGa > 1:3357877/1‑71 (MQ=255) tCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGa > 1:83728/1‑71 (MQ=255) aTTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGAc < 1:2213906/70‑1 (MQ=255) ttcttcAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGAc > 1:1760407/1‑69 (MQ=255) ttCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATcgacga < 1:544886/49‑1 (MQ=255) ttCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAg < 1:914307/50‑1 (MQ=255) ccTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTATGAc > 1:4157636/1‑60 (MQ=255) cTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATc < 1:424816/41‑1 (MQ=255) cTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTc < 1:3642581/63‑1 (MQ=255) cTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAGa < 1:1329069/70‑1 (MQ=255) | TGATGGATAAGCTGGATGTGATCCTGCTGATGTCCGTCAACCCTGGTTTCGGCGGTCAGTCTTTCATTCCTCAAACACTGGATAAACTGCGCGAAGTACGTCGCCGTATCGACGAGTCTGGCTTTGACATTCGACTAGA > W3110S.gb/4125744‑4125882 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |