| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,208,832 | A→G | 26.7% | H126R (CAC→CGC) | yrdD → | predicted DNA topoisomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,208,832 | 0 | A | G | 26.7% | 92.9 / 40.6 | 60 | H126R (CAC→CGC) | yrdD | predicted DNA topoisomerase |
| Reads supporting (aligned to +/- strand): ref base A (19/25); new base G (6/10); total (25/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.39e-01 | |||||||||||
GCAATTACATGCCCCCAATGTCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAACTTCAAACCCATAGCTGGAGAATGCCCT > W3110S.gb/4208762‑4208899 | gCAATTACATGCCCCCAATGTCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCa > 1:861525/1‑71 (MQ=255) ttACATGCCCCCAATGTCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCActct > 1:4531173/1‑71 (MQ=255) tACATGCCCCCAATGTCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCtt > 1:119675/1‑71 (MQ=255) aCATGCCCCCAATGTCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTtg > 1:99479/1‑71 (MQ=255) aTGCCCCCAATGTCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTtgtg < 1:365968/71‑1 (MQ=255) aTGCCCCCAATGTCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTtgtg < 1:2859070/71‑1 (MQ=255) tGCCCCCAATGTCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTtgtg < 1:185138/70‑1 (MQ=255) cccccAATGTCGGACGGGCCATCTGGTCCAGCGCCGGTCCCGTTATGGCAAAACATTTCGCTCTTGTGa < 1:4366635/69‑1 (MQ=255) ccAATGTCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTTGTGATCGCt < 1:4474369/71‑1 (MQ=255) cAATGTCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTTGTGATCg > 1:4573490/1‑68 (MQ=255) cAATGTCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTTGTGATCGCTa < 1:3612325/71‑1 (MQ=255) aTGTCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCGCTCtt < 1:3999077/59‑1 (MQ=255) aTGTCGGACGGGCCATCTGGTCCAGCGCAGCTCCCGTTATGGGAAAACATTTCa > 1:2353509/1‑54 (MQ=255) tCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTTGTGATCGCTAcc > 1:4623708/1‑68 (MQ=255) gCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCGCTCTTGTGATCGCTACCCGGAGTGTCaa < 1:3780247/71‑1 (MQ=255) gCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCaa < 1:512465/71‑1 (MQ=255) ccATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCGCTCTTGTGATCGCTACCCGGAGTGTCAAt > 1:3110082/1‑71 (MQ=255) ccATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTTGTGATCGCTACCCgg < 1:4038234/62‑1 (MQ=255) ccATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTTGTGATCGCTACCCgg < 1:3226550/62‑1 (MQ=255) aTCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTTGTGATCGCTACCCGGAg > 1:3424425/1‑62 (MQ=255) tCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCGCTCTTGTGATCGCTa < 1:707906/54‑1 (MQ=255) cTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCGCTCTTGTGATCGCTACCCGGAGTGTCAATTTGc > 1:285331/1‑71 (MQ=255) cTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCGCTCTTGTGATCGCTACCCGGAGTGTCAATTTGc > 1:4341309/1‑71 (MQ=255) tGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCa > 1:1581754/1‑37 (MQ=255) tGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCGCTCTTGTGATCGCTACCCGGAGTGTCAATTTGc > 1:2017769/1‑70 (MQ=255) tGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTTGTGAt > 1:2546343/1‑47 (MQ=255) tGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGc > 1:2304039/1‑70 (MQ=255) gTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGc < 1:701663/68‑1 (MQ=255) tCCAGCGCCGCTCCCGTTATGGCAAAACATTTCGCTCTtg < 1:409608/40‑1 (MQ=255) tCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCAtt < 1:294726/71‑1 (MQ=255) tCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCAtt < 1:3868828/71‑1 (MQ=255) cAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTaa > 1:2134684/1‑71 (MQ=255) aGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAAc < 1:54247/71‑1 (MQ=255) gcgcCGCTCCCGTTATGGCAAAACATTTCGCTCTtgtg < 1:2025157/38‑1 (MQ=255) cgccgcTCCCGTTATGGCAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCa < 1:1036516/56‑1 (MQ=255) gccgcTCCCGTTATGGCAAAACATTTCACTCTtgt > 1:4508182/1‑35 (MQ=255) gccgcTCCCGTTATGGCAAAACATTTCACTCTTGTGATCGCTACCCgg < 1:3877803/48‑1 (MQ=255) cgcTCCCGTTATGGCAAAACATTTCACTCTTGTGATCGCTCCCCGGAGTGTCAATTTGCCATTAACTTCa > 1:3315109/1‑70 (MQ=255) gcTCCCGTTATGGCAAAACATTTCACTCTTGTGATc > 1:3595690/1‑36 (MQ=255) cTCCCGTTATGGCAAAACATTTCACTCTTGTGATCGCTACCCgg < 1:550146/44‑1 (MQ=255) cccGTTATGGCAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCAAtt > 1:3743082/1‑52 (MQ=255) ccGTTATGGCAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCaa < 1:647907/49‑1 (MQ=255) ccGTTATGGCAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAACTTCAAAccc < 1:645850/70‑1 (MQ=255) ccGTTATGGCAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAACTTCAAAccc < 1:2513653/70‑1 (MQ=255) gTTATGGCAAAACATTTCGCTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCa > 1:2890494/1‑54 (MQ=255) ttATGGCAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCAAt < 1:2770659/47‑1 (MQ=255) ttATGGCAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAACTTCa < 1:1117655/62‑1 (MQ=255) ttATGGCAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAACTTCa < 1:285490/62‑1 (MQ=255) tATGGCAAAACATTTCGCTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAACTTCAAACCCATAGc > 1:3993211/1‑71 (MQ=255) tATGGCAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAACTTCAAACCCATAGc > 1:982961/1‑71 (MQ=255) gCAAAACATTTCGCTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAACTTCAAACCCATAGCTGga < 1:652945/71‑1 (MQ=255) gCAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAACTTCAAACCCATAGCTGga < 1:1240990/71‑1 (MQ=255) cAAAACATTTCGCTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAACTTCAAACCCATAGCTGgag < 1:60206/71‑1 (MQ=255) aaaaCATTTCACTCTTGTGATCGCTACCCGGAGTGTCaa > 1:785972/1‑39 (MQ=255) aaaCATTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAACTTCAAACCCATAGCTGGAGaa < 1:844622/71‑1 (MQ=255) aaCATTTCGCTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAACTTCAAACCCATAGCTGgag < 1:1138807/68‑1 (MQ=255) aaCATTTCGCTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAACTTCAAACCCATAGCTGGAGAAt < 1:3146497/71‑1 (MQ=255) aCATTTCACTCTTGTGATCGCTACCCGGAGTGTCAAtt > 1:647389/1‑38 (MQ=255) cATTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAACTTCAAACCCATAGCTGGAGAATGc > 1:2422615/1‑71 (MQ=255) aTTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCa > 1:3997331/1‑41 (MQ=255) aTTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAACTTCAAACCCATAGCTGGAGAATGcc < 1:4472834/71‑1 (MQ=255) tttCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAACTTCAAACCCATAGCTGGAGAATGccc < 1:2918562/71‑1 (MQ=255) tCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAACTTCAAACCCATAGCTGGAGAATGCCCt > 1:7925/1‑70 (MQ=255) | GCAATTACATGCCCCCAATGTCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAACTTCAAACCCATAGCTGGAGAATGCCCT > W3110S.gb/4208762‑4208899 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |