| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,229,470 | A→G | 14.3% | Q684R (CAG→CGG) | metH → | homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,229,470 | 0 | A | G | 14.3% | 65.3 / 4.6 | 28 | Q684R (CAG→CGG) | metH | homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent |
| Reads supporting (aligned to +/- strand): ref base A (10/14); new base G (2/2); total (12/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01 | |||||||||||
TGGAATACTCGCTGGTCAAAGGCATTACCGAGTTTATCGAGCAGGATACCGAAGAAGCCCGCCAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTG > W3110S.gb/4229407‑4229540 | tGGAATACTCGCTGGTCAAAGGCATTACCGAGTTTATCGAGCAGGATACCGAAGAAGCCCGCCAGCAGGc > 1:1729533/1‑70 (MQ=255) gAATACTCGCTGGTCAAAGGCATTACCGAGTTTATCGAGCAGGATACCGAAGAAGCCCGCCAGCAGGc < 1:3217941/68‑1 (MQ=255) gAATACTCGCTGGTCAAAGGCATTACCGAGTTTATCGAGCAGGATACCGAAGAAGCCCGCCAGCAGGCt < 1:2892257/69‑1 (MQ=255) gAATACTCGCTGGTCAAAGGCATTACCGAGTTTATCGAGCAGGATACCGAAGAAGCCCGCCAGCAGGCTa > 1:2632766/1‑70 (MQ=255) aaTACTCGCTGGTCAAAGGCATTACCGAGTTTATCGAGCAGGATACCGAAGAAGCCCGCCAGCAGGCTAcg < 1:2159486/71‑1 (MQ=255) gCTGGTCAAAGGCATTACCGAGTTTATCGAGCAGGATACCGAAGAAGCCCGCCAGCAGGCTACGCGCCCg < 1:2082162/70‑1 (MQ=255) tCAAAGGCATTACCGAGTTTATCGAGCAGGATACCGAAGAAGCCCGCCAGCAGGCTACGCGCCCGATTg > 1:1927712/1‑69 (MQ=255) cAAAGGCATTACCGAGTTTATCGAGCAGGATACCGAAGAAGCCCGCCAGCAGGCTACGCGCCCGATTg > 1:4119698/1‑68 (MQ=255) aaGGCATTACCGAGTTTATCGAGCAGGATACCGAAGAAGCCCGCCAGCAGGCTACGCGCCCGATTGAAGTg < 1:1719637/71‑1 (MQ=255) cATTACCGAGTTTATCGAGCAGGATACCGAAGAAGCCCGCCGGCAGGCTACGCGCCCGATTGAAGTGATTg < 1:357997/71‑1 (MQ=255) cATTACCGAGTTTATCGAGCAGGATACCGAAGAAGCCCGCCAGCAGGCTACGCGCCCGATTGAAGTGATTg < 1:311780/71‑1 (MQ=255) aCCGAGTTTATCGAGCAGGATACCGAAGAAGCCCGCCAGCAGGCTACGCGCCCGATTGAAGTGATTGAAgg > 1:2290444/1‑71 (MQ=255) aCCGAGTTTATCGAGCAGGATACCGAAGAAGCCCGCCAGCAGGCTACGCGCCCGATTGAAGTGATTGAAgg > 1:865840/1‑71 (MQ=255) ccGAGTTTATCGAGCAGGATACCGAAGAAGCCCGCCAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGc < 1:3684204/71‑1 (MQ=255) tttATCGAGCAGGATACCGAAGAAGCCCGCCAGCAGGCTACGCGCCCGATTGAAGTGATTg < 1:4223437/61‑1 (MQ=255) tttATCGAGCAGGATACCGAAGAAGCCCGCCAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGtt > 1:2766733/1‑71 (MQ=255) ttATAGAGCAGGATACCGAAGAAGCCCGCCAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGtt < 1:4200430/70‑1 (MQ=255) tATCGAGCAGGATACCGAAGAAGCCCGCCGGCAGGCTACGCGCCCGATTGAAGTGATTGaa > 1:3294542/1‑61 (MQ=255) tATCGAGCAGGATACCGAAGAAGCCCGCCGGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTg < 1:2065994/70‑1 (MQ=255) tATCGAGCAGGATACCGAAGAAGCCCGCCAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGa > 1:751862/1‑71 (MQ=255) gAGCAGGATACCGAAGAAGCCCGCCGGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGa > 1:1261536/1‑71 (MQ=255) tACCGAAGAAGCCCGCCAGCAGGCTACGCGCCCGATTGAAGTGATTGaa > 1:1498222/1‑49 (MQ=255) tACCGAAGAAGCCCGCCAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATg > 1:128152/1‑70 (MQ=255) tACCGAAGAAGCCCGCCAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGa < 1:4440383/71‑1 (MQ=255) ccGAAGAAGCCCGCCAGCAGGCTACGCGCCCGATTGAAGTGATTg < 1:94760/45‑1 (MQ=255) gaagCCCGCCAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGt < 1:4059660/71‑1 (MQ=255) aagCCCGCCAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCa < 1:1342999/60‑1 (MQ=255) agCCCGCCAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCg < 1:1354650/71‑1 (MQ=255) agcagGCTCCGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTg > 1:508350/1‑71 (MQ=255) agcagGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTg > 1:1717128/1‑71 (MQ=255) | TGGAATACTCGCTGGTCAAAGGCATTACCGAGTTTATCGAGCAGGATACCGAAGAAGCCCGCCAGCAGGCTACGCGCCCGATTGAAGTGATTGAAGGCCCGTTGATGGACGGCATGAATGTGGTCGGCGACCTG > W3110S.gb/4229407‑4229540 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |