| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,243,626 | C→T | 5.5% | intergenic (+304/‑291) | yjbH → / → yjbA | predicted porin/predicted phosphate starvation inducible protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,243,626 | 0 | C | T | 5.5% | 231.0 / 2.7 | 72 | intergenic (+304/‑291) | yjbH/yjbA | predicted porin/predicted phosphate starvation inducible protein |
| Reads supporting (aligned to +/- strand): ref base C (29/39); new base T (2/2); total (31/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01 | |||||||||||
CCAGCATAAGCAGGAAGAACAGAGACTGATAACATCAAAGCAGCAAAATAAGGCTTCATTTTTACCACCTTTATCAGGTTACGTTTCATTTGTTCCAGAGGAACATTGTCGATTTTTCGCGCATTGCTGGT > W3110S.gb/4243559‑4243689 | ccAGCATAAGCAGGAAGAACAGAGACTGATAACATCAAAGCAGCAAAATAAGGCTTCATTTTTACCACCtt > 1:3082943/1‑71 (MQ=255) cAGCATAAGCAGGAAGAACAGAGACTGATAACATCAAAGCAGCAAAATAAGGCTTCATTTTTACCACCtt > 1:2092328/1‑70 (MQ=255) gCATAAGCAGGAAGAACAGAGACTGATAACATCAAAGCAGCAAAATAAGGCTTCATTTTTACCATCTTTAt > 1:3128507/1‑71 (MQ=255) gCATAAGCAGGAAGAACAGAGACTGATAACATCAAAGCAGCAAAATAAGGCTTCATTTTTACCACCTTTa > 1:2806780/1‑70 (MQ=255) cATAAGCAGGAAGAACAGAGACTGATAACATCAAAGCAGCAAAATAAGGCTTCATTTTTACCACCTTTAt > 1:555946/1‑70 (MQ=255) aTAAGCAGGAAGAACAGAGACTGATAACATCAAAGCAGCAAAATAAGGCTTCATTTTTACCACCTTTATCa < 1:4451915/71‑1 (MQ=255) tAAGCAGGAAGAACAGAGACTGATAACATCAAAGCAGCAAAATAAGGCTTCATTTTTACCACCTTTa > 1:2554856/1‑67 (MQ=255) aGCAGGAAGAACAGAGACTGATAACATCAAAGCAGCAAAATAAGGCTTCATTTTTACCACCTTTATCAGGt > 1:866622/1‑71 (MQ=255) aGCAGGAAGAACAGAGACTGATAACATCAAAGCAGCAAAATAAGGCTTCATTTTTACCACCTTTATCAGGt < 1:632678/71‑1 (MQ=255) cAGGAAGAACAGAGACTGATAACATCAAAGCAGCAAAATAAGGCTTCATTTTTACCACCTTTATCAGGTTa < 1:1106581/71‑1 (MQ=255) aGGAAGAACAGAGACTGATAACATCAAAGCAGCAAAATAAGGCTTCATTTTTACCACCTTTAt > 1:4221465/1‑63 (MQ=255) gaagaaCAGAGACTGATAACATCAAAGCAGCAAAATAAGGCTTCATTTTTACCACCTTTATCAGGTTa < 1:357759/68‑1 (MQ=255) aagaaCAGAGACTGATAACATCAAAGCAGCAAAATAAGGCTTCATTTTTACCACCTTTATCAGGTTACGtt < 1:2182484/71‑1 (MQ=255) agaaCAGAGACTGATAACATCAAAGCAGCAAAATAAGGCTTCATTTTTACCACCTTTATCAGGTTACGttt > 1:443571/1‑71 (MQ=255) aaCAGAGACTGATAACATCAAAGCAGCAAAATAAGGCTTCATTTTTACCACCTTTATCAGGTTACGTTTCa < 1:1112256/71‑1 (MQ=255) cAGAGACTGATAACATCAAAGCAGCAAAATAAGGCTTCATTTTTACCACCTTTATCAGGTTACGTTTCAtt > 1:3569628/1‑71 (MQ=255) cAGAGACTGATAACATCAAAGCAGCAAAATAAGGCTTCATTTTTAACATCTTTATCAGGTTACGTTTCAtt < 1:4096838/71‑1 (MQ=255) gagaCTGATAACATCAAAGCAGCAAAATAAGGCTTCATTTTTACCACCTTTATCAGGTTACGTTTCATTTg > 1:1963059/1‑71 (MQ=255) gagaCTGATAACATCAAAGCAGCAAAATAAGGCTTCATTTTTACCACCTTTATCAGGTTACGTTTCATTTg > 1:2781890/1‑71 (MQ=255) cTGATAACATCAAAGCAGCAAAATAAGGCTTCATTTTTACCACCTTTAt < 1:2785710/49‑1 (MQ=255) cTGATAACATCAAAGCAGCAAAATAAGGCTTCATTTTTACCACCTTTAt < 1:1036337/49‑1 (MQ=255) tGATAACATCAAAGCAGCAAAATAAGGCTTCATTTTTACCACCTTTATCAGGTTACGTTTCAtt > 1:2373628/1‑64 (MQ=255) tGATAACATCAAAGCAGCAAAATAAGGCTTCATTTTTACCACCTTTATCAGGTTACGTTTCATTTGt > 1:2757021/1‑67 (MQ=255) aTAACATCAAAGCAGCAAAATAAGGCTTCATTTTTACCATCTTTATCAGGTTACGTTTCATTTGTTCCa < 1:2160894/69‑1 (MQ=255) aTAACATCAAAGCAGCAAAATAAGGCTTCATTTTTACCACCTTTATCAGGTTACGTTTCATTTGTTc < 1:3802126/67‑1 (MQ=255) aTAACATCAAAGCAGCAAAATAAGGCTTCATTTTTACCACCTTTATCAGGTTACGTTTCATTTGTTCCa < 1:747120/69‑1 (MQ=255) aTAACATCAAAGCAGCAAAATAAGGCTTCATTTTTACCACCTTTATCAGGTTACGTTTCATTTGTTCCa < 1:2641672/69‑1 (MQ=255) aTAACATCAAAGCAGCAAAATAAGGCTTCATTTTTACCACCTTTATCAGGTTACGTTTCATTTGTTCCa < 1:2647256/69‑1 (MQ=255) aTAACATCAAAGCAGCAAAATAAGGCTTCATTTTTACCACCTTTATCAGGTTACGTTTCATTTGTTCCa > 1:3928574/1‑69 (MQ=255) aaCATCAAAGCAGCAAAATAAGGCTTCATTTTTACCACCTTTATCAGGTTa > 1:1197304/1‑51 (MQ=255) aCATCAAAGCAGCAAAATAAGGCTTCATTTTTACCACCTTTATCAGGTTACGTTTCATTTGTTCCAGAgg > 1:2612820/1‑70 (MQ=255) aTCAAAGCAGCAAAATAAGGCTTCATTTTTACCACCTTTATCAGGTTACGTTTCa < 1:4096852/55‑1 (MQ=255) aTCAAAGCAGCAAAATAAGGCTTCATTTTTACCACCTTTATCAGGTTACGTTTCATTTGTTCCAGAGGAAc < 1:4109279/71‑1 (MQ=255) tCAAAGCAGCAAAATAAGGCTTCATTTTTACCACCTTTATCAGGTTACGTTTCATTTGTTCCAGAGGaa < 1:2231471/69‑1 (MQ=255) tCAAAGCAGCAAAATAAGGCTTCATTTTTACCACCTTTATCAGGTTACGTTTCATTTGTTCCAGAGGAACa > 1:4544179/1‑71 (MQ=255) tCAAAGCAGCAAAATAAGGCTTCATTTTTAACATCTTTATCAGGTTAc > 1:2283828/1‑48 (MQ=255) aaaGCAGCAAAATAAGGCTTCATTTTTACCACCTTTATCa > 1:824481/1‑40 (MQ=255) aaaGCAGCAAAATAAGGCTTCATTTTTACCACCTTTATCa > 1:4416339/1‑40 (MQ=255) aaaGCAGCAAAATAAGGCTTCATTTTTACCACCTTTATCAGGTTACGTTTCATTTGTTCCAGAGGAACAtt < 1:2322026/71‑1 (MQ=255) aaaGCAGCAAAATAAGGCTTCATTTTTACCACCTTTATCAGGTTACGTTTCATTTGTTCCAGAGGAACAtt < 1:3030038/71‑1 (MQ=255) gcagcaAAATAAGGCTTCATTTTTACCACCTTTATCAGGTTACGTTTCATTTGTTCCAGAGGAACATTGTc < 1:3781253/71‑1 (MQ=255) agcaAAATAAGGCTTCATTTTTACCACCTTTATCAGGTTACGTTTCAtt > 1:1698392/1‑49 (MQ=255) caATATAAGGCTTCATTTTTACCACCTTTATCAGGTTACGTTTCATTTGTTCCAGAGGAACATTGTCGAt < 1:1761228/70‑1 (MQ=255) aaaaTAAGGCTTCATTTTTACCACCTTTATCAGGTTACGTTTCATTTGTTCCAGAGGAACAt > 1:2439893/1‑62 (MQ=255) aaaaTAAGGCTTCATTTTTACCACCTTTATCAGGTTACGTTTCATTTGTTCCAGAGGAACATTGTCGAttt < 1:1051450/71‑1 (MQ=255) aaaTAAGGCTTCATTTTTACCACCTTTATCAGGTTACGTTTCATTTGTTc > 1:2844604/1‑50 (MQ=255) aaaTAAGGCTTCATTTTTACCACCTTTATCAGGTTACGTTTCATTTGTTCCAGAGGAACATTGTCGAtttt < 1:3841709/71‑1 (MQ=255) aaaTAAGGCTTCATTTTTACCACCTTTATCAGGTTACGTTTCATTTGTTCCAGAGGAACATTGTCGAttt > 1:2984214/1‑70 (MQ=255) aaaTAAGGCTTCATATTTACCACCTTTATGAGGTTACGTTTCATTTGTTCCAGAGGAAc < 1:44540/59‑1 (MQ=255) aaTAAGGCTTCATTTTTACCACCTTTATCAGGTTACGttt < 1:2253459/40‑1 (MQ=255) aaTAAGGCTTCATTTTTACCACCTTTATCAGGTTACGTTTCATTTGTTCCAGAGGAACATTGTCGAttttt < 1:3316328/71‑1 (MQ=255) ggCTTCATTTTTACCACCTTTATCAGGTTACGTTTCATTTGTTCCAGAGGAACATTGTCGATTTTTCgcgc < 1:2215393/71‑1 (MQ=255) cTTCATTTTTACCACCTTTATCAGGTTACGTTTCATTTGTTCCAGAGGAACATTGTCGATTTTTCGCGCAt < 1:1123545/71‑1 (MQ=255) tCATTTTTACCACCTTTATCAGGTTACGTTTCATTTGTTCCa < 1:2342477/42‑1 (MQ=255) tCATTTTTACCACCTTTATCAGGTTACGTTTCATTTGTTCCAGAGGAACATTGTCTAt > 1:4460215/1‑58 (MQ=255) cgtttttACCACCTTTATCAGGTTACGTTTCATTTGTTCCAGAGGAACATTGTCGAt < 1:2697331/55‑1 (MQ=255) cATTTTTACCACCTTTATCAGGTTACGTTTCATTTGTTCCAGAGGAACATTGTCGAt < 1:822433/57‑1 (MQ=255) cATTTTTACCACCTTTATCAGGTTACGTTTCATTTGTTCCAGAGGAACATTGTCGATTTTTCGCGCAtt < 1:2518227/69‑1 (MQ=255) cATTTTTACCACCTTTATCAGGTTACGTTTCATTTGTTCCAGAGGAACATTGTCGATTTTTCGCGCATTg < 1:1023885/70‑1 (MQ=255) cATTTTTACCACCTTTATCAGGTTACGTTTCATTTGTTCCAGAGGAACATTGTCGATTTTTCGCGCATTGc > 1:2551299/1‑71 (MQ=255) aTTTTTACCACCTTTATCAGGTTACGTTTCATTTGTTCCAGAGGAACATTGTCGATTTTTCGCGCATTGc < 1:1778852/70‑1 (MQ=255) aTTTTTACCACCTTTATCAGGTTACGTTTCATTTGTTCCAGAGGAACATTGTCGATTTTTCGCGCATTGCt > 1:4055292/1‑71 (MQ=255) aTTTTTACCACCTTTATCAGGTTACGTTTCATTTGTTCCAGAGGAACATTGTCGATTTTTCGCGCATTGCt < 1:3349309/71‑1 (MQ=255) aTTTTTACCACCTTTATCAGGTTACGTTTCATTTGTTCCAGAGGAACATTGTCGATTTTTCGCGCATTGCt > 1:552298/1‑71 (MQ=255) aTTTTTACCACCTTTATCAGGTTACGTTTCATTTGTTCCAGAGGAACATTGTCGATTTTTCGCGCATTGCt < 1:1226585/71‑1 (MQ=255) tttttACCACCTTTATCAGGTTACGTTTCATTTGTTCCAGAGTAAc < 1:586787/46‑1 (MQ=255) tttttACCACCTTTATCAGGTTACGTTTCATTTGTTCCAGAGGAACATTGTc > 1:2105005/1‑52 (MQ=255) tttttACCACCTTTATCAGGTTACGTTTCATTTGTTCCAGAGGAACATTGTCGATTTTTcgcg < 1:163318/63‑1 (MQ=255) tttttACCACCTTTATCAGGTTACGTTTCATTTGTTCCAGAGGAACATTGTCGATTTTTcgcg < 1:2704187/63‑1 (MQ=255) tttACCACCTTTATCAGGTTACGTTTCATTTGTTCCAGAGGAACATTGTCGATTTTTCGCGCATTGCtggt > 1:2223775/1‑71 (MQ=255) tttACCACCTTTATCAGGTTACGTTTCATTTGTTCCAGAGGAACATTGTCGATTTTTCGCGCATTGCtgg < 1:4432236/70‑1 (MQ=255) tttACCACCTTTATCAGGTTACGTTTCATTTGTTCCAGAGGAACATTGTCGATTTTTCGCGCATTGCtgg < 1:647354/70‑1 (MQ=255) ccTTTATCAGGTTACGTTTCATTTGTTCCAGAGGAAc < 1:4597270/37‑1 (MQ=255) ccTTTATCAGGTTACGTTTCATTTGTTCCAGAGGAACATTGTCGAttt < 1:2245888/48‑1 (MQ=255) | CCAGCATAAGCAGGAAGAACAGAGACTGATAACATCAAAGCAGCAAAATAAGGCTTCATTTTTACCACCTTTATCAGGTTACGTTTCATTTGTTCCAGAGGAACATTGTCGATTTTTCGCGCATTGCTGGT > W3110S.gb/4243559‑4243689 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |