| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,249,681 | A→C | 15.3% | F111V (TTC→GTC) | malE ← | maltose transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,249,681 | 0 | A | C | 15.3% | 77.8 / 17.1 | 46 | F111V (TTC→GTC) | malE | maltose transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (20/19); new base C (2/5); total (22/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.58e-01 | |||||||||||
GGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTCCTGGAACGCTTTGTCCGGGGTGATTTCAGCCAACAGGCCAGATTGAGCGTAGCCACCAAAGCGGTCGTG > W3110S.gb/4249615‑4249744 | ggTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTCCTGGAACGCt < 1:3986661/71‑1 (MQ=255) aaGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTCCTGGACCGCttt < 1:3605787/70‑1 (MQ=255) aaTCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTCCTGGAACGCTTTGTCCg < 1:3365244/71‑1 (MQ=255) aTCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTCCTGGAACGCt < 1:3749334/63‑1 (MQ=255) aTCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTCCTGGAACGCt < 1:2387690/63‑1 (MQ=255) cAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTCCTGGAACGCttt > 1:2442645/1‑63 (MQ=255) aGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTCCTGGACCGCtt < 1:786528/61‑1 (MQ=255) aGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTCCTGGAACGCTTTGTCCgg > 1:2989492/1‑68 (MQ=255) aGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTCCTGGAACGCTTTGTCCGGGGt > 1:3307987/1‑71 (MQ=255) aGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTCCTGGAACGCTTTGTCCGGGGt < 1:3046257/71‑1 (MQ=255) aGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTCCTGGAACGCTTTGTCCGGGGt < 1:283391/71‑1 (MQ=255) aGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTCCTGGAACGCTTTGTCCGGGGt > 1:1043622/1‑71 (MQ=255) aGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTCCTGGAACGATTTGTCCGGGGt > 1:3362649/1‑71 (MQ=255) aGCTTGCCGTTGTAACGTACGGCATCCAAGGTAAACGGATACAGCTTGTCCTGGAACGCTTTGTCCGGGGt > 1:2534876/1‑71 (MQ=255) gCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTCCTGGAACGCTTTGTCCGGGGTGAtt < 1:2086104/70‑1 (MQ=255) ccGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTCCTGGACCGCTTTGTCCGGGGTGAttt < 1:2518369/70‑1 (MQ=255) gTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTCCTGGAACGCTTTGTCCGGGGTGATTTCAg > 1:2507356/1‑71 (MQ=255) ttGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTCCTGGAACGCTTTGTc < 1:1521743/56‑1 (MQ=255) gTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTCCTGGAACGCTTTGTCCGGGGTGATTTCATcc < 1:1576283/70‑1 (MQ=255) gTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTCCTGGAACGCTTTGTCCGGGGTGATTTCAGcc < 1:4244461/70‑1 (MQ=255) tAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTCCTGGAACGCTTTGTCCGGGGTGATTTCAGCCa < 1:476636/70‑1 (MQ=255) aCGTACGGCATCCCAGGTAAACGGATACAGCTTGTCCTGGACCGCTTTGTCCGGGGTGATTTCAGCCAAc < 1:3862153/70‑1 (MQ=255) cGGCATCCCAGGTAAACGGATACAGCTTGTCCTGGAACGCttt > 1:4361963/1‑43 (MQ=255) cGGCATCCCAGGTAAACGGATACAGCTTGTCCTGGAACGCTTTGTcc > 1:3764504/1‑47 (MQ=255) gCATCCCAGGTAAACGGATACAGCTTGTCCTGGAACGCTTTGTCCGGGGTGAt < 1:2629143/53‑1 (MQ=255) cccAGGTAAACGGATACAGCTTGTCCTGGAACGCTTTGTCCgg > 1:3915867/1‑43 (MQ=255) ccAGGTAAACGGATACAGCTTGTCCTGGAACGCTTTGTCCGGGGTGATTTCAGCCAACAGGCCAGa > 1:786125/1‑66 (MQ=255) cAGGTAAACGGATACAGCTTGTCCTGGAACGCTTTGTCCGGGGTGATTTCAGCCAACAGGCCAGATTGAGc > 1:4505284/1‑71 (MQ=255) aGGTAAACGGATACAGCTTGTCCTGGAACGCTTTGTCCGGGGTGAt < 1:3540306/46‑1 (MQ=255) ggTAAACGGATACAGCTTGTCCTGGAACGCTTTGTCCGGGGTGATTTCAGCCAACa < 1:1121212/56‑1 (MQ=255) ggTAAACGGATACAGCTTGTCCTGGAACGCTTTGTCCGGGGTGATTTCAGCCAACAGGCCAGATTGAGCg > 1:595304/1‑70 (MQ=255) ggTAAACGGATACAGCTTGTCCTGGAACGCTTTGTCCGGGGTGATTTCAGCCAACAGGCCAGATTGAGCGt < 1:1590992/71‑1 (MQ=255) tAAACGGATACAGCTTGTCCTGGACCGCTTTGTCCggg > 1:4583625/1‑38 (MQ=255) aaaCGGATACAGCTTGTCCTGGAACGCTTTGTCCGGGGTGATTTc > 1:3041084/1‑45 (MQ=255) ggATACAGCTTGTCCTGGAACGCTTTGTCCGGGGTGATTTCAGCCAACAGGCCAGATTGAGCGTAGccac > 1:1505502/1‑70 (MQ=255) tACAGCTTGTCCTGGAACGCTTTGTCCGGGGTGATTTCAGCCAAc < 1:3915642/45‑1 (MQ=255) tACAGCTTGTCCTGGAACGCTTTGTCCGGGGTGATTTCAGCCAACAGGCCAGATTGAGCGTAGCCACCaaa < 1:4517381/71‑1 (MQ=255) tACAGCTTGTCCTGGAACGCTTTGTCCGGGGTGATTTCAGCCAACAGGCCAGATTGAGCGTAGCCACCaaa < 1:1478433/71‑1 (MQ=255) tACAGCTTGTCCTGGAACGCTTTGTCCGGGGTGATTTCAGCCAACAGGCCAGATTGAGCGTAGCCACCaa > 1:936939/1‑70 (MQ=255) aCAGCTTGTCCTGGAACGCTTTGTCCGGGGTGATTTCAGCCAACAGGCCAGATTGAGCGTa < 1:3921809/61‑1 (MQ=255) cAGCTTGTCCTGGAACGCTTTGTCCGGGGTGATTTCAGCCAAc > 1:2406762/1‑43 (MQ=255) cAGCTTGTCCTGGAACGCTTTGTCCGGGGTGATTTCAGCCAACAg > 1:2853195/1‑45 (MQ=255) gCTTGTCCTGGACCGCTTTGTCCGGGGTGATTTCAGCCAACAGGCCAGATTGAGCGTAGCCACCAAAGCgg > 1:549229/1‑71 (MQ=255) cTTGTCCTGGAACGCTTTGTCCGGGGTGATTTCAGCCAACAGGCCAGATTGAGCGTAGCCACCAAAGCGGt > 1:2443277/1‑71 (MQ=255) tCCTGGACCGCTTTGTCCGGGGTGATTTCAGCCAACAGGCCAGATTGAGCGTAGCCACCAAAGCGGTCgtg < 1:3145597/71‑1 (MQ=255) ggAACGCTTTGTCCGGGGTGATTTCAGCCAACAGGCCAGATTGAGCGTAGCCACCAAAGCg > 1:746100/1‑61 (MQ=255) aCGCTTTGTCCGGGGTGATTTCAGCCAACAGGCCAGATTGAGCg > 1:3812993/1‑44 (MQ=255) | GGTAAGCAATCAGCTTGCCGTTGTAACGTACGGCATCCCAGGTAAACGGATACAGCTTGTCCTGGAACGCTTTGTCCGGGGTGATTTCAGCCAACAGGCCAGATTGAGCGTAGCCACCAAAGCGGTCGTG > W3110S.gb/4249615‑4249744 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |