| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,325,315 | T→A | 28.0% | K10M (AAG→ATG) | phnI ← | carbon‑phosphorus lyase complex subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,325,315 | 0 | T | A | 28.0% | 61.9 / 24.1 | 39 | K10M (AAG→ATG) | phnI | carbon‑phosphorus lyase complex subunit |
| Reads supporting (aligned to +/- strand): ref base T (14/14); new base A (8/3); total (22/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
AGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGG > W3110S.gb/4325249‑4325385 | aGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTtc < 1:3095600/69‑1 (MQ=255) cGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGccc > 1:2799534/1‑71 (MQ=255) cGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGccc > 1:2047447/1‑71 (MQ=255) aaaTCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCttt < 1:4066922/71‑1 (MQ=255) gCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACg < 1:1441130/70‑1 (MQ=255) gCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACg > 1:1610134/1‑70 (MQ=255) gCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACg < 1:2327798/70‑1 (MQ=255) gCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCATCTCGCCCCCTTTCACGGCAACg > 1:918411/1‑70 (MQ=255) gCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCATCTCGCCCCCTTTCACGGCAACg > 1:3311513/1‑70 (MQ=255) gCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCATCTCGCCCCCTTTCACGGCAACg > 1:3729334/1‑70 (MQ=255) gCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCATCTCGCCCCCTTTCACGGCAACg > 1:454136/1‑70 (MQ=255) gtcGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCATCTCGCCCCCTTTCACGGCAACGTACATc < 1:1723946/69‑1 (MQ=255) ggCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCaa > 1:2878693/1‑63 (MQ=255) ggCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACa < 1:2063943/69‑1 (MQ=255) ggCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACAt > 1:719392/1‑70 (MQ=255) gCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTtc < 1:2295842/43‑1 (MQ=255) gCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCATCTCGCCCCCTTTCACGGCAACg < 1:789512/64‑1 (MQ=255) ggCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCATCTCGCCCCCTTTCa > 1:2767341/1‑51 (MQ=255) ctctTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTc > 1:3872363/1‑71 (MQ=255) ctctTGCAGGGCGTGGGCGGCGTCGATCGCCATCTCGCCCCCTTTCACGGCAACGTACATCAGCacac > 1:1720023/1‑68 (MQ=255) tctTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACAt < 1:488318/59‑1 (MQ=255) tGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCcaca > 1:194357/1‑71 (MQ=255) gCAGGGCGT‑GGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACAt < 1:13305/70‑1 (MQ=255) cAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACAt < 1:1156602/70‑1 (MQ=255) cAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACAt < 1:3331064/70‑1 (MQ=255) cGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTgg > 1:2619957/1‑71 (MQ=255) tGGGCGGCGTCGATCGCCATCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGa > 1:336271/1‑65 (MQ=255) gcggcgTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTcgc > 1:3863592/1‑71 (MQ=255) gcgTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACa < 1:1859509/40‑1 (MQ=255) gcgTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGa < 1:4272843/71‑1 (MQ=255) gTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACa < 1:3018253/38‑1 (MQ=255) gTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGaa > 1:2485667/1‑70 (MQ=255) gTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATAAGCACACCTCcaca > 1:3343624/1‑54 (MQ=255) tCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAAt < 1:2967684/70‑1 (MQ=255) tCGATCGCCATCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATa < 1:2874653/71‑1 (MQ=255) gATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCacac > 1:3988494/1‑44 (MQ=255) gATCGCCTTCTCGCCCCCTTTCAAGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGc > 1:1861687/1‑71 (MQ=255) gATCGCCATCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGc > 1:1882686/1‑71 (MQ=255) cGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCcag > 1:272140/1‑71 (MQ=255) ttCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAgg > 1:2568424/1‑71 (MQ=255) | AGTTCGGGCAAATCGGTATCGCCTCGGCGTCGGCTCTCTTGCAGGGCGTGGGCGGCGTCGATCGCCTTCTCGCCCCCTTTCACGGCAACGTACATCAGCACACCTCCACATGAGTGGTTCGCGGAATAGCCAGCAGG > W3110S.gb/4325249‑4325385 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |