| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,354,061 | C→T | 7.5% | G218G (GGG→GGA) | dcuR ← | DNA‑binding response regulator in two‑component regulatory system with DcuS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,354,061 | 0 | C | T | 7.5% | 164.7 / 3.3 | 53 | G218G (GGG→GGA) | dcuR | DNA‑binding response regulator in two‑component regulatory system with DcuS |
| Reads supporting (aligned to +/- strand): ref base C (26/23); new base T (2/2); total (28/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01 | |||||||||||
CCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATGAGGTATT > W3110S.gb/4353992‑4354125 | ccGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGcc > 1:1005130/1‑71 (MQ=255) aTTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGt < 1:331823/70‑1 (MQ=255) aTTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGt > 1:3117771/1‑70 (MQ=255) aTTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGt > 1:4172439/1‑70 (MQ=255) atatTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGTCCCGTGACGCCAt < 1:1887845/71‑1 (MQ=255) atatTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCAt < 1:4248137/71‑1 (MQ=255) tatTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCAt > 1:2437324/1‑70 (MQ=255) tatTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCAt < 1:1860181/70‑1 (MQ=255) tatTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATa < 1:2825292/71‑1 (MQ=255) gTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATg < 1:538063/70‑1 (MQ=255) tagtgagtagtgTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTg < 1:2623679/70‑1 (MQ=255) agtgagtagtgTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGAt < 1:2971686/65‑1 (MQ=255) agtagtgTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATaa > 1:2079369/1‑56 (MQ=255) tgtTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGa > 1:941544/1‑43 (MQ=255) tgtTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGa > 1:164708/1‑43 (MQ=255) tgtTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATaa > 1:3883381/1‑51 (MQ=255) tgtTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATa < 1:3488771/50‑1 (MQ=255) tgtTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATg < 1:215965/53‑1 (MQ=255) tgtTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACa < 1:154985/67‑1 (MQ=255) tgtTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGAt > 1:1538015/1‑71 (MQ=255) ttCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGAtgt > 1:3129270/1‑71 (MQ=255) cTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCAt > 1:2426907/1‑45 (MQ=255) cTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGa > 1:3467321/1‑66 (MQ=255) cTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTgg > 1:2099502/1‑71 (MQ=255) tGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGa > 1:170535/1‑59 (MQ=255) ccTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATa > 1:44288/1‑50 (MQ=255) ccTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGc < 1:4506097/69‑1 (MQ=255) cTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAg < 1:445314/70‑1 (MQ=255) cTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAg < 1:318369/70‑1 (MQ=255) tGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTgg > 1:4471717/1‑52 (MQ=255) gAATACGGTAACGGTAAACTGGACGTCCCGTGACGCAATAATGGATACTGGTGAACAAGATGTGGCAGtt > 1:3498050/1‑70 (MQ=255) gAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATa < 1:1367288/40‑1 (MQ=255) aaTACGGTAACGGTAAACTGGACGTCCCGTGACGCCATAATGGATACTGGTGAACAAGAtg > 1:3510339/1‑61 (MQ=255) aaTACGGTAACGGTAAACTGGACGCCCCGTGACGCCAt < 1:781996/38‑1 (MQ=255) aaTACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCa < 1:3067829/66‑1 (MQ=255) aTACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGTACAAGATGTGGCAGTTg < 1:2379276/69‑1 (MQ=255) tACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGa > 1:641636/1‑69 (MQ=255) acggtaacggtaAACTGGACGCCCCGTGACGCCATAATg < 1:210466/39‑1 (MQ=255) cggtaacggtaaACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAg > 1:1902121/1‑63 (MQ=255) gtaacggtaaACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGAccagc > 1:159818/1‑70 (MQ=255) taacggtaaACTGGACGCCCCGTGACTCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGAccagcca < 1:3364557/71‑1 (MQ=255) aacggtaaACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGAccagc > 1:3528762/1‑68 (MQ=255) acggtaaACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGAccagccag < 1:4492900/70‑1 (MQ=255) gtaaACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGa > 1:1838582/1‑68 (MQ=255) taaACTGGACGCCCCGTGACGCCATAATGGATACTGGTTAACAAGATGTGGCAGTTGACCAGCCAGATGAg < 1:926536/71‑1 (MQ=255) taaACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGAt > 1:192337/1‑68 (MQ=255) aaaCTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAg > 1:780366/1‑44 (MQ=255) aaCTGGCCGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATGAgg > 1:62086/1‑70 (MQ=255) aaCTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATGAg > 1:4637666/1‑69 (MQ=255) aCTGGACGTCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGc < 1:510727/49‑1 (MQ=255) cTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGAtg > 1:117031/1‑44 (MQ=255) cTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGa > 1:463150/1‑63 (MQ=255) tGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTg < 1:605260/52‑1 (MQ=255) gACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATGAGGTAtt < 1:2675728/69‑1 (MQ=255) | CCGTTATTGGCAATATTGTTTCAGTAGTGAGTAGTGTTCTGCCTGAATACGGTAACGGTAAACTGGACGCCCCGTGACGCCATAATGGATACTGGTGAACAAGATGTGGCAGTTGACCAGCCAGATGAGGTATT > W3110S.gb/4353992‑4354125 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |