| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,361,585 | C→T | 35.8% | P571P (CCG→CCA) | cadA ← | lysine decarboxylase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,361,585 | 0 | C | T | 35.8% | 82.4 / 80.7 | 81 | P571P (CCG→CCA) | cadA | lysine decarboxylase 1 |
| Reads supporting (aligned to +/- strand): ref base C (21/31); new base T (16/13); total (37/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.48e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.83e-01 | |||||||||||
GTGGATATTCTGAGCCAGTTCCTGAATACGCATGTTTTCATAGAATTCAGGATCTTCACGATACAGAGACGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACGCACGTTTAAAGTCAGTCAGAGCACGCAGC > W3110S.gb/4361516‑4361651 | gtgGATATTCTGAGCCAGTTCCTGAATACGCATGTTTTCATAGAATTCAGGATCTTCACGATACAGAGAc < 1:1298913/70‑1 (MQ=255) atatTCTGAGCCAGTTCCTGAACACGCATGTTTTCATAGAATTCAGGATCTTCACGATACAGAGACGgcag < 1:1274892/71‑1 (MQ=255) ttCTGAGCCAGTTCCTGAATACGCATGTTTTCATAGAATTCAGGATCTTCACGATACAGAGATGGCAGCAt < 1:1051181/71‑1 (MQ=255) ttCTGAGCCAGTTCCTGAATACGCATGTTTTCATAGAATTCAGGATCTTCACGATACAGAGATGGCAGCAt < 1:2069289/71‑1 (MQ=255) ttCTGAGCCAGTTCCTGAATACGCATGTTTTCATAGAATTCAGGATCTTCACGATACAGAGATGGCAGCAt > 1:880487/1‑71 (MQ=255) cTGAGCCAGTTCCTGAATACGCATGTTTTCATAGAATTCAGGATCTTCACGATACAGAGACGGCAGCATGt > 1:1557609/1‑71 (MQ=255) aGCCAGTTCCTGAATACGCATGTTTTCATAGAATTCAGGATCTTCACGATACAGAGACGGCAGCATGtttt > 1:3140495/1‑71 (MQ=255) aGCCAGTTCCTGAATACGCATGTTTTCATAGAATTCAGGATCTTCACGATACAGAGACGGCAGCATGttt < 1:1049280/70‑1 (MQ=255) ccAGTTCCTGAATACGCATGTTTTCATAGAATTCAGGATCTTCACGATACAGAGATGGCAGCATGTTTTTc < 1:1843558/71‑1 (MQ=255) ccAGTTCCTGAATACGCATGTTTTCATAGAATTCAGGATCTTCACGATACAGAGACGGCAGCATGttttt < 1:362470/70‑1 (MQ=255) cAGTTCCTGAATACGCATGTTTTCATAGAATTCAGGATCTTCACGATACAGAGATGGCAGCATGTTTTTca > 1:3100891/1‑71 (MQ=255) gTTCCTGAATACGCATGTTTTCATAGAATTCAGGATCTTCACGATACAGAGACGGCAGCATGTTTTTca < 1:1594592/69‑1 (MQ=255) tCCTGAATACGCATGTTTTCATAGAATTCAGGATCTTCACGATACAGAGATGGCAGCATGttttt > 1:2226242/1‑65 (MQ=255) tCCTGAATACGCATGTTTTCATAGAATTCAGGATCTTCACGATACAGAGACGGCAGCATGTTTTTca > 1:1641579/1‑67 (MQ=255) cTGAATACGCATGTTTTCATAGAATTCAGGATCTTCACGATACAGAGACGgcagca < 1:4432676/56‑1 (MQ=255) cTGAATACGCATGTTTTCATAGAATTCAGGATCTTCACGATACAGAGACGgcagca < 1:630703/56‑1 (MQ=255) cTGAATACGCATGTTTTCATAGAATTCAGGATCTTCACGATACAGAGACGGCAGCATGTTTTTCACACGc < 1:4267598/70‑1 (MQ=255) gAATACGCATGTTTTCATAGAATTCAGGATCTTCACGATACAGAGATGGCAGCATGtt > 1:1378172/1‑58 (MQ=255) gAATACGCATGTTTTCATAGAATTCAGGATCTTCACGATACAGAGACGgcagca < 1:60448/54‑1 (MQ=255) aaTACGCATGTTTTCATAGAATTCAGGATCTTCACGATACAGAGACGGCAGCATGTTTTTCACACGCAGGt > 1:1980052/1‑71 (MQ=255) aTACGCATGTTTTCATAGAATTCAGGATCTTCACGATACAGAGATGGCAGCATGTTTTTca > 1:2310410/1‑61 (MQ=255) aTACGCATGTTTTCATAGAATTCAGGATCTTCACGATACAGAGACGGCAGCATGTTTTTCACACGCAGGtt < 1:1435167/71‑1 (MQ=255) cATGTTTTCATAGAATTCAGGATCTTCACGATACAGAGACGgcagca > 1:3827447/1‑47 (MQ=255) cATGTTTTCATAGAATTCAGGATCTTCACGATACAGAGACGGCAGCATGTTTTTCACACGCAg > 1:31195/1‑63 (MQ=255) cATGTTTTCATAGAATTCAGGATCTTCACGATACAGAGACGGCAGCATGTTTTTCACACGCAGGTTCAGGt > 1:1068270/1‑71 (MQ=255) tGTTTTCATAGAATTCAGGATCTTCACGATACAGAGATGGCAGCATGttt < 1:1476549/50‑1 (MQ=255) tGTTTTCATAGAATTCAGGATCTTCACGATACAGAGATGGCAGCATGttt < 1:2842401/50‑1 (MQ=255) tGTTTTCATAGAATTCAGGATCTTCACGATACAGAGACGGCAGCATGTTTTTCACACGCAGGTTCAg < 1:3308826/67‑1 (MQ=255) tGTTTTCATAGAATTCAGGATCTTCACGATACAGAGACGGCAGCATGTTTTTCACACGCAGGTTCAGGTCg < 1:273661/71‑1 (MQ=255) tGTTTTCATAGAATTCAGGATCTTCACGATACAGAGACGGCAGCATGTTTTTCACACGCAGGTTCAGGTCg < 1:446700/71‑1 (MQ=255) tGTTTTCATAGAATTCAGGATCTTCACGATACAGAGACGGCAGCATGTTTTTCACACGCAGGTTCAGGTCg < 1:4110158/71‑1 (MQ=255) gTTTTCATAGAATTCAGGATCTTCACGATACAGAGATGGCAGCATGttt < 1:2847864/49‑1 (MQ=255) gTTTTCATAGAATTCAGGATCTTCACGATACAGAGACGGCAGCATGttttt < 1:3045763/51‑1 (MQ=255) gTTTTCATAGAATTCAGGATCTTCACGATACAGAGACGGCAGCATGTTTTTCACACGCAGGTTCAGGTCg > 1:1151517/1‑70 (MQ=255) ttttCATAGAATTCAGGATCTTCACGATACAGAGATGGCAGCATGTTTTTCACACGCAGGTTCAg < 1:4135637/65‑1 (MQ=255) ttttCATAGAATTCAGGATCTTCACGATACAGAGACGGCAGCATGt > 1:3757809/1‑46 (MQ=255) ttttCATAGAATTCAGGATCTTCACGATACAGAGACGGCAGCATGTTTTTcac < 1:641888/53‑1 (MQ=255) ttttCATAGAATTCAGGATCTTCACGATACAGAGACGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGaa > 1:3363486/1‑71 (MQ=255) ttttCATAGAATTCAGGATCTTCACGATACAGAGACGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGaa > 1:3255720/1‑71 (MQ=255) ttttCATAGAATTCAGGATCTTCACGATACAGAGACGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGaa > 1:3181020/1‑71 (MQ=255) ttttAATAGAATTCAGGATCTTCACGATACAGAGATGGCAGCATGTTTTTCACACGCa > 1:2634383/1‑58 (MQ=39) ttCATAGAATTCAGGATCTTCACGATACAGAGATGGCAGCATGttt > 1:3529962/1‑46 (MQ=255) tCATAGAATTCAGGATCTTCACGATACAGAGACGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACg < 1:4291839/70‑1 (MQ=255) cATAGAATTCAGGATCTTCACGATACAGAGATGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACGCa > 1:1660187/1‑71 (MQ=255) tAGAATTCAGGATCTTCACGATACAGAGATGGCAGCATGtt < 1:3326784/41‑1 (MQ=255) tAGAATTCAGGATCTTCACGATACAGAGATGGCAGCATGtt < 1:3865300/41‑1 (MQ=255) aGAATTCAGGATCTTCACGATACAGAGACGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACGCACGt > 1:4210704/1‑71 (MQ=255) aaTTCAGGATCTTCACGATACAGAGACGGCAGCATGTTTTTCACACGCa < 1:784360/49‑1 (MQ=255) ttCAGGATCTTCACGATACAGAGATGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGa < 1:688595/59‑1 (MQ=255) ttCAGGATCTTCACGATACAGAGACGGCAGCATGTTTTTCACACGCAg > 1:443783/1‑48 (MQ=255) ttCAGGATCTTCACGATACAGAGACGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACGCACGTTTaa < 1:3736698/71‑1 (MQ=255) cAGGATCTTCACGATACAGAGATGGCAGCATGTTTTTCacac > 1:42730/1‑42 (MQ=255) cAGGATCTTCACGATACAGAGACGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACGCACGTTTAAag > 1:2231150/1‑71 (MQ=255) cAGGATCTTCACGATACAGAGACGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACGCACGTTTAAag > 1:4116003/1‑71 (MQ=255) aGGATCTTCACGATACAGAGACGGCAGCATGttttt < 1:253420/36‑1 (MQ=255) aTCTTCACGATACAGAGACGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACGCACGTTTa < 1:4382093/64‑1 (MQ=255) tCTTCACGATACAGAGATGGCAGCATGTTTTTCacac > 1:1696099/1‑37 (MQ=255) tCTTCACGATACAGAGATGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACGCACGTTTAAagtcagt > 1:957887/1‑71 (MQ=255) cTTCACGATACAGAGATGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGaa > 1:2845335/1‑52 (MQ=255) cTTCACGATACAGAGACGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACGCa < 1:804359/56‑1 (MQ=255) cTTCACGATACAGAGACGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACGCACGTTTaaa > 1:3167975/1‑64 (MQ=255) ttCACTATACAGAGACGGCAGCATGTTTTTCACACGCAGGt < 1:4632480/41‑1 (MQ=255) ttCACGATACAGAGATGGCAGCATGTTTTTCACACGCAGGTTc > 1:2519472/1‑43 (MQ=255) ttCACGATACAGAGATGGCAGCATGTTTTTCACACGCAGGTTc > 1:3850277/1‑43 (MQ=255) ttCACGATACAGAGATGGCAACATGTTTTTCACACGCAGGTTc > 1:4442965/1‑43 (MQ=38) tCACGATACAGAGACGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACGCACGTTTAAagtcagt > 1:3537584/1‑68 (MQ=255) cACGATACAGAGATGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACGCACGTTTAAAgtcagtca > 1:3641168/1‑69 (MQ=255) cACGATACAGAGACGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACg > 1:4092250/1‑51 (MQ=255) cACGATACAGAGACGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACg > 1:4044436/1‑51 (MQ=255) cACGATACAGAGACGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACGCACGTTTAAagtcagt < 1:3323914/67‑1 (MQ=255) cACGATACAGAGACGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACGCACGTTTAAag > 1:41067/1‑62 (MQ=255) cACGATACAGAGACGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACGCACGTTTAAAgtcagtca < 1:101141/69‑1 (MQ=255) aCGATACAGAGACGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACGCACGTTTAAAGTCAGTCagag < 1:2420750/71‑1 (MQ=255) aCGATAAAGAGATGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACGCACGTTTAAAGTCAGTCaga < 1:4300127/70‑1 (MQ=255) cGATACAGAGACGGCAGCATGTTTTTCACACGCAGGtt < 1:1808541/38‑1 (MQ=255) gATACAGAGACGGCAGCATGTTTTTCACACGCAGGtt < 1:1093980/37‑1 (MQ=255) tACAGAGACGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACGCACGTTTa < 1:3153361/54‑1 (MQ=255) gagaTGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACGCACGTTTAAAGTCAGTCAGAGCACgcagc < 1:2487682/71‑1 (MQ=255) gagaCGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACGCACGTTTAAAGTCAGTCAGAGCACgcagc < 1:516322/71‑1 (MQ=255) gagaCGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACGCACGTTTAAAGTCAGTCAGAGCACgcagc < 1:2941970/71‑1 (MQ=255) agaTGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACGCACGTTTa < 1:1116564/49‑1 (MQ=255) gaCGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGa < 1:3632643/37‑1 (MQ=255) | GTGGATATTCTGAGCCAGTTCCTGAATACGCATGTTTTCATAGAATTCAGGATCTTCACGATACAGAGACGGCAGCATGTTTTTCACACGCAGGTTCAGGTCGAACGCACGTTTAAAGTCAGTCAGAGCACGCAGC > W3110S.gb/4361516‑4361651 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |