| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,411,479 | C→T | 12.7% | G48G (GGC→GGT) | rnr → | exoribonuclease R, RNase R |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,411,479 | 0 | C | T | 12.7% | 129.8 / 6.4 | 47 | G48G (GGC→GGT) | rnr | exoribonuclease R, RNase R |
| Reads supporting (aligned to +/- strand): ref base C (20/21); new base T (2/4); total (22/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGAACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTT > W3110S.gb/4411411‑4411544 | gAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGAACTGCACATTGAAGGTGa < 1:3641253/71‑1 (MQ=255) aaCATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATTAGCTGGCGGTAGAACTGCACATTGAAGGCg < 1:895322/69‑1 (MQ=255) aCATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGAACTGCACATTGAAGGCGAag < 1:38678/71‑1 (MQ=255) aCATTTAACCAAACGTGAAAAACCGCCCAGCCGTGATGAGCTGGCGGTAGAACTGCACATTGAAGGCg > 1:3829710/1‑68 (MQ=255) tAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGGACTGCACATTGAAGGCGAAGagca < 1:816628/70‑1 (MQ=255) cAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGAACTGCACATTGAAGGCGAAGAGCAGCTTg < 1:1036729/71‑1 (MQ=255) aCGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGAACTGCACATTGAAGGCGAAGAGCAGCTTGAAg < 1:68731/71‑1 (MQ=255) aaaaaCCGGCCAGCCGTGATGAGCTGGCGGTAGAACTGCACATTGAAGGTGAAGAGCAGCTTGAAGGCCTg > 1:1176186/1‑71 (MQ=255) aaaaaCCGGCCAGCCGTGATGAGCTGGCGGTAGAACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTg > 1:3860033/1‑71 (MQ=255) aaaaaCCGGCCAGCCGTGATGAGCTGGCGGTAGAACTGCACATTGAAGGCGAAGAGAAGCTTGAAGGCCTg > 1:503836/1‑71 (MQ=255) aaaCCGGCCAGCCGTGATGAGCTGGCGGTAGAACTGCACATTGAAGGTGAAGAGCAGCTTGAAGGCCTGCg > 1:1180220/1‑71 (MQ=255) aaaCCGGCCAGCCGTGATGAGCTGGCGGTAGAACTGCACATTGAAGGCGaa > 1:1934104/1‑51 (MQ=255) aaaCCGGCCAGCCGTGATGAGCTGGCGGTAGAACTGCACATTGAAGGCGAAGAGCAGCt > 1:3942839/1‑59 (MQ=255) cGGCCAGCCGTGATGAGCTGGCGGTAGAACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTcgc > 1:632215/1‑71 (MQ=255) cGGCCAGCCGTGATGAGCTGGCGGTAGAACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTcgc > 1:2760516/1‑71 (MQ=255) ccAGCCGTGATGAGCTGGCGGTAGAACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTcgccgc < 1:3393024/71‑1 (MQ=255) ccGTGATGAGCTGGCGGTAGAACTGCACATTGAAGGTGa < 1:4125506/39‑1 (MQ=255) cGTGATGAGCTGGCGGTAGAACTGCACATTGAAGGCGAAGAGCAGCTTGaa > 1:164906/1‑51 (MQ=255) tgatgaGCTGGCGGTAGAACTGCACATTGAAGGCGAAGAGCAGCTTg < 1:1932086/47‑1 (MQ=255) atgaGCTGGCGGTAGAACTGCACATTGAAGGTGAAGAGCAGCTT‑AAGGCCTGCGTCGCCGCCTgcgcgc < 1:2570500/69‑1 (MQ=255) atgaGCTGGCGGTAGAACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTgcgcgc > 1:3255048/1‑70 (MQ=255) atgaGCTGGCGGTAGAACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTgcgcgc > 1:3878654/1‑70 (MQ=255) atgaGCTGGCGGTAGAACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTgcgcgc < 1:3252354/70‑1 (MQ=255) tgaGCTGGCGGTAGAACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCt < 1:2275864/51‑1 (MQ=255) aGCTGGCGGTAGAACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGAt < 1:1396546/70‑1 (MQ=255) aGCTGGCGGTAGAACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATg < 1:2324779/71‑1 (MQ=255) gCTGGCGGTAGAACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATg > 1:801007/1‑70 (MQ=255) cGGTAGAACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAgcgc < 1:443285/71‑1 (MQ=255) cGGTAGAACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAgcgc < 1:4440353/71‑1 (MQ=255) gTAGAACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGAt < 1:2993675/62‑1 (MQ=255) gTAGAACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGAt < 1:92006/62‑1 (MQ=255) gTAGAACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAgcgc < 1:1067050/69‑1 (MQ=255) aGAACTGCACATTGAAGGCGAAGAGCAGCTTGAAg < 1:1305725/35‑1 (MQ=255) aGAACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTcgc > 1:552742/1‑46 (MQ=255) aGAACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTcgc > 1:963983/1‑46 (MQ=255) aGAACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATg > 1:1267541/1‑71 (MQ=255) gAACTGCACATTGAAGGTGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAgcgc < 1:1771286/66‑1 (MQ=255) gAACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATgg > 1:2407797/1‑71 (MQ=255) caTTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTgcgcgc < 1:3166358/48‑1 (MQ=255) caTTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTgg > 1:4018700/1‑71 (MQ=255) caTTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTgg > 1:4023998/1‑71 (MQ=255) ttGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTgcgcgc < 1:489365/46‑1 (MQ=255) gAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATg > 1:1636918/1‑48 (MQ=255) gAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCtt > 1:2788506/1‑71 (MQ=255) gAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCtt > 1:1104657/1‑71 (MQ=255) aaGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCtt < 1:1091823/70‑1 (MQ=255) gCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTg < 1:360606/62‑1 (MQ=255) | GAACATTTAACCAAACGTGAAAAACCGGCCAGCCGTGATGAGCTGGCGGTAGAACTGCACATTGAAGGCGAAGAGCAGCTTGAAGGCCTGCGTCGCCGCCTGCGCGCGATGGAGCGCGATGGTCAACTGGTCTT > W3110S.gb/4411411‑4411544 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |