| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,440,755 | T→C | 7.1% | K165E (AAA→GAA) | cpdB ← | 2':3'‑cyclic‑nucleotide 2'‑phosphodiesterase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,440,755 | 0 | T | C | 7.1% | 152.5 / 2.9 | 56 | K165E (AAA→GAA) | cpdB | 2':3'‑cyclic‑nucleotide 2'‑phosphodiesterase |
| Reads supporting (aligned to +/- strand): ref base T (28/24); new base C (2/2); total (30/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTCCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGAC > W3110S.gb/4440692‑4440824 | ccATTATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTCCGTCTTTATCGAcc < 1:1784056/71‑1 (MQ=255)ccATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTCCGTCTTTATCGAcc < 1:3529318/71‑1 (MQ=255)ccATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTCCGTCTTTATCGAcc < 1:1918022/71‑1 (MQ=255) tGATTTGTGGTGGCACGACGCCAATATAGACAATCTTCAGCGTCTGTTTTTTTCCGTCTTTATCGACCACt > 1:2328792/1‑71 (MQ=255) aTTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTCCGTCTTTATCGACCACTTc > 1:2456518/1‑71 (MQ=255) gtggtgGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTCCGTCTTTATCGACCACTTCGgtg > 1:1378498/1‑71 (MQ=255) gtggCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTCCGTCTTTATCGACCACTTCGGTGTCt > 1:1544540/1‑71 (MQ=255) gCACGCCGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTCCGTCTTTATCGACCACTTCGGTGTCttt > 1:1462559/1‑70 (MQ=255) gCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTCCGTCTTTATCGACCACTTCGGTGTCttt < 1:4093764/70‑1 (MQ=255) gCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTCCGTCTTTATCGAACACTTCGGTGTCttt > 1:3761416/1‑70 (MQ=255) acgacgCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTCCGTCTTTATCGACCACTTCGGTGTCTttaat > 1:1831330/1‑71 (MQ=255) cgacgCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTCCGTCTTTATCGACCACTTCTGTGTCTttattt < 1:1499493/71‑1 (MQ=255) gacgCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTCCGTCTTTATCGACCACTTCGGTGTCTttaatta < 1:1525317/71‑1 (MQ=255) aaTATAGCCAATCTTCAGCGTCTGTTTTTTTCCGTCTTTATCGa > 1:128415/1‑44 (MQ=255) atatAGCCAATCTTCAGCGTCTGTTTTTTTCCGTCTTTa > 1:3091883/1‑39 (MQ=255) atatAGCCAATCTTCAGCGTCTGTTTTTTTCCGTCTTTATCGACCACTTCGGTGTCTTCAATTaaa < 1:136049/66‑1 (MQ=255) taGCCAATCTTCAGCGTCTGTTTTTTTCCGTCTTTATCGACCACTTCGGTGTCTttaatt < 1:3157728/60‑1 (MQ=255) taGCCAATCTTCAGCGTCTGTTTTTTTCCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATa < 1:828849/65‑1 (MQ=255) taGCCAATCTTCAGCGTCTGTTTTTTTCCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGgtg > 1:1570651/1‑70 (MQ=255) ccAATCTTCAGCGTCTGTTTTTTTCCGTCTTTATCGACCACTTCGGTGTCTTTAATTaaa < 1:3272409/60‑1 (MQ=255) ccAATCTTCAGCGTCTGTTTTTTTCCGTCTTTATCGACCACTTCGGTGTCTTTAATTaaa < 1:2038791/60‑1 (MQ=255) ccAATCTTCAGCGTCTGTTTTTTTCCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTaaa < 1:4585613/71‑1 (MQ=255) cAATCTTCAGCGTCTGTTTTTTTCCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTaaa > 1:822096/1‑70 (MQ=255) cAATCTTCAGCGTCTGTTTTTTTCCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAAc < 1:1720295/71‑1 (MQ=255) cAATCTTCAGCGTCTGTTTTTTTCCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAAc < 1:420530/71‑1 (MQ=255) aaTCTTCAGCGTCTGTTTTTTTCCGTCTTTATCGACCACTTCgg > 1:2025639/1‑44 (MQ=255) tCTTCAGCGTCTGTTTTTTTCCGTCTTCATCGACCACTTCGGTGTCttt < 1:2468624/49‑1 (MQ=255) tCTTCAGCGTCTGTTTTTTTCCGTCTTCATCGACCACTTCGGTGTCttt < 1:2474035/49‑1 (MQ=255) cTTCAGCGTCTGTTTTTTTCCGTCTTTATCGACCACTTCGGTGTCTttaa > 1:2225167/1‑50 (MQ=255) cTTCAGCGTCTGTTTTTTTCCGTCTTCATCGACCa > 1:2220476/1‑35 (MQ=255) cTTCAGCGTCTGTTTTTTTCCGTCTTCATCGACCa > 1:1464639/1‑35 (MQ=255) ttCAGCGTCTGTTTTTTTCCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTaaa > 1:1084174/1‑65 (MQ=255) ttCAGCGTCTGTTTTTTTCCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTgg > 1:124945/1‑71 (MQ=255) ttCAGCGTCTGTTTTTTTCCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTgg > 1:3565208/1‑71 (MQ=255) ttCAGCGTCTGTTTTTTTCCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTg > 1:1645326/1‑70 (MQ=255) gCGTCTGTTTTTTTCCGTCTTTATCGACCACTTCGGTGTCTTTAATTaaa < 1:926018/50‑1 (MQ=255) gTCTGTTTTTTTCCGTCTTTATCGACCACTTCGgt < 1:4624962/35‑1 (MQ=255) tCTGTTTTTTTCCGTCTTTATCGACCTCTTCGGTGTCtt < 1:3938020/39‑1 (MQ=255) cTGTTTTTTTCCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTgg > 1:801294/1‑71 (MQ=255) tGTTTTTTTCCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGtt < 1:4503444/67‑1 (MQ=255) tGTTTTTTTCCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGt > 1:3627539/1‑71 (MQ=255) gTTTTTTTCCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGtt > 1:4107143/1‑71 (MQ=255) gTTTATTTCCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGt > 1:3988641/1‑70 (MQ=255) tttttttCCGTCTTTATCGACCACTTCGGTGTCTttaa > 1:2010532/1‑38 (MQ=255) tttttttCCGTCTTTATCGACCACTTCGGTGTCTttaa < 1:1341302/38‑1 (MQ=255) tttttttCCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTg < 1:322568/59‑1 (MQ=255) tttttCCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTg > 1:423193/1‑71 (MQ=255) ttttCCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGtt > 1:391076/1‑67 (MQ=255) ttttCCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTg < 1:837189/70‑1 (MQ=255) ttCCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGc < 1:820181/70‑1 (MQ=255) ccGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGtt > 1:2622370/1‑63 (MQ=255) ccGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGtt > 1:794406/1‑63 (MQ=255) gTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTgg > 1:3469079/1‑65 (MQ=255) tCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAAt < 1:1409814/71‑1 (MQ=255) tCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAAt < 1:1805371/71‑1 (MQ=255) cTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCaa > 1:751792/1‑69 (MQ=255) tttATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGtt > 1:3708345/1‑53 (MQ=255) tttATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTc > 1:2142819/1‑59 (MQ=255) tttATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGa > 1:4162724/1‑71 (MQ=255) tttATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGa > 1:1776485/1‑71 (MQ=255) tttATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGa > 1:1047903/1‑71 (MQ=255) ttATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGAc > 1:1342314/1‑71 (MQ=255) | CCATGATTTGTGGTGGCACGACGCCAATATAGCCAATCTTCAGCGTCTGTTTTTTTCCGTCTTTATCGACCACTTCGGTGTCTTTAATTAAATACGGTGTAAACATTGGCTGTTTGGTTCTGGCGTCAATGAC > W3110S.gb/4440692‑4440824 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |