| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,464,055 | A→T | 22.4% | intergenic (+62/‑180) | pmbA → / → cybC | predicted peptidase required for the maturation and secretion of the antibiotic peptide MccB17/ECK4231:JW4195:b4236; cytochrome b562, truncated |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,464,055 | 0 | A | T | 22.4% | 97.0 / 25.1 | 49 | intergenic (+62/‑180) | pmbA/cybC | predicted peptidase required for the maturation and secretion of the antibiotic peptide MccB17/ECK4231:JW4195:b4236; cytochrome b562, truncated |
| Reads supporting (aligned to +/- strand): ref base A (22/16); new base T (6/5); total (28/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGACAGTAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAG > W3110S.gb/4463985‑4464123 | ggACAGTAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACGCAAAATCATTCGCACTGCa > 1:3482955/1‑71 (MQ=255)ggACAGTAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCa < 1:1069378/71‑1 (MQ=255) cAGTAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCTTc > 1:1805779/1‑70 (MQ=255) cAGTAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATc > 1:2535018/1‑70 (MQ=255) cAGTAAAAAAACTGGCGCGACATGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATc > 1:3030306/1‑70 (MQ=255) gTAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATc > 1:67818/1‑68 (MQ=255) tAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCTTCgaga > 1:3029174/1‑71 (MQ=255) tAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCgaga > 1:391410/1‑71 (MQ=255) aaaaCTGGCGCGACCTGCCACGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCTTCGAGACGGa < 1:2706649/71‑1 (MQ=255) aaCTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGa > 1:799823/1‑69 (MQ=255) aCTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCTTCGAGACg < 1:3460029/66‑1 (MQ=255) gACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAg > 1:3364580/1‑71 (MQ=255) cTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTg < 1:2214918/62‑1 (MQ=255) gCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCg > 1:4437136/1‑71 (MQ=255) cTATAAACTAAAAAATTACACAAAATCATTCGCACTGCTTCGAGACGGAAAGTGAATGAGGGCCGGGGAGc > 1:1338068/1‑71 (MQ=255) ataAACTAAAAAATTACACAAAATCATTCGCACTGCTTCGAGACGGAAAGTGAATGAGGGCCGGGGAGCg > 1:801282/1‑70 (MQ=255) ataAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGaa > 1:3471404/1‑54 (MQ=255) ataAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGc > 1:2939152/1‑69 (MQ=255) ataAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCg > 1:4079410/1‑70 (MQ=255) taAACTAAAAAATTACACAAAATCATTCGGACTGCTTCgaga > 1:336580/1‑42 (MQ=38) taAACTAAAAAATTACACAAAATCATTCGCACTGCTTCGAGACGGAAAGTGAATGAGGGCCGGGGAGCg < 1:3147579/69‑1 (MQ=255) taAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGa < 1:4535935/46‑1 (MQ=255) taAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTg < 1:2695392/51‑1 (MQ=255) aaaCTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTAc > 1:2612859/1‑71 (MQ=255) aaCTAAAAAATTACACAAAATCATTCGCACTGCTTCga > 1:54912/1‑38 (MQ=255) aaCTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGaa > 1:787630/1‑51 (MQ=255) aaCTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGaa > 1:3413300/1‑51 (MQ=255) aaCTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGaa > 1:3089511/1‑51 (MQ=255) tAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCg > 1:3305593/1‑70 (MQ=255) aaaaaaTTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGaa > 1:4459808/1‑47 (MQ=255) aaaaaTTACACAAAATCATTCGCACTGCATCGAGACGGaa < 1:605858/40‑1 (MQ=255) aaaaaTTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAAt > 1:557630/1‑47 (MQ=255) aaaaaTTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGc < 1:3174645/69‑1 (MQ=255) aaaaTTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGt < 1:3328805/71‑1 (MQ=255) aaaaTTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGt < 1:3841443/71‑1 (MQ=255) aaaTTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGa > 1:2440883/1‑43 (MQ=255) ttACACAAAATCATTCTCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGt < 1:1445216/71‑1 (MQ=255) ttACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGt < 1:492209/71‑1 (MQ=255) aTCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTa > 1:2241478/1‑50 (MQ=255) aTCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTc > 1:1465758/1‑71 (MQ=255) aTTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGa < 1:1718445/70‑1 (MQ=255) ttCGCACTGCTTCGAGACGGTAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAAt < 1:3368836/71‑1 (MQ=255) ttCGCACTGCTTCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAAt < 1:4258942/71‑1 (MQ=255) ttCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAAt < 1:4293236/71‑1 (MQ=255) ttCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAAt < 1:4374478/71‑1 (MQ=255) gCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGa < 1:949597/66‑1 (MQ=255) cTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCg > 1:3481702/1‑70 (MQ=255) tGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTa < 1:1110574/39‑1 (MQ=255) tGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTa < 1:4436322/48‑1 (MQ=255) gCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAg > 1:860525/1‑71 (MQ=255) cATCGAGACGGAAAGTGAATGAGGGCC‑GGGATCGTACTCGc < 1:932743/41‑1 (MQ=37) | GGACAGTAAAAAAACTGGCGCGACCTGCCGCGCTATAAACTAAAAAATTACACAAAATCATTCGCACTGCATCGAGACGGAAAGTGAATGAGGGCCGGGGAGCGTACTCGCAGTACGTGACCCGGTCGAATGAGCGTAG > W3110S.gb/4463985‑4464123 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |