| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 286,496 | T→C | 15.1% | F162L (TTT→CTT) | yagH → | predicted xylosidase/arabinosidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 286,496 | 0 | T | C | 15.1% | 76.6 / 7.7 | 33 | F162L (TTT→CTT) | yagH | predicted xylosidase/arabinosidase |
| Reads supporting (aligned to +/- strand): ref base T (15/13); new base C (2/3); total (17/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.56e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01 | |||||||||||
TACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGGCACGCCGCTCTGCTACA > W3110S.gb/286427‑286565 | tACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGtt > 1:3348920/1‑71 (MQ=255) cTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGttt < 1:1936027/70‑1 (MQ=255) atatCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACATCATCGTGTTACAGGCGTTTGAc > 1:2958473/1‑71 (MQ=255) tCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCg > 1:284745/1‑71 (MQ=255) aCCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCg > 1:150122/1‑68 (MQ=255) ggggCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGCTTGACCCGCAGACCGGCACg < 1:4247043/70‑1 (MQ=255) gggCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGAACGct < 1:2648254/71‑1 (MQ=255) ccGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTg < 1:4436849/49‑1 (MQ=255) ccacAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCa < 1:313095/48‑1 (MQ=255) ccacAGCAACCCGCACAACACCATCGTGTTACAGGCGCTTg < 1:1220441/41‑1 (MQ=255) ccacAGCAACCCGCACAACACCATCGTGTTACAGGCGCTTg < 1:2712778/41‑1 (MQ=255) cacaGCAACCCGCACAACACCATCGTGTCACAGGCGTTTGACCCGCAGACCGGCACGctct < 1:657280/61‑1 (MQ=255) acaGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGAc > 1:2278680/1‑41 (MQ=255) acaGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGAc > 1:4005176/1‑41 (MQ=255) caGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAg < 1:3378054/46‑1 (MQ=255) aCCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCACCCGAGCGCAAAACg < 1:1921013/71‑1 (MQ=255) ccGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGct < 1:2316503/50‑1 (MQ=255) ccGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCt < 1:3278409/71‑1 (MQ=255) cGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTg < 1:3169384/71‑1 (MQ=255) gCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGt > 1:2791884/1‑71 (MQ=255) cacaACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGtt < 1:3461146/71‑1 (MQ=255) caacaCCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGctc > 1:2943850/1‑46 (MQ=255) caacaCCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTa < 1:1180299/71‑1 (MQ=255) acCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCgg > 1:1436740/1‑71 (MQ=255) tCGTGTTACAGGCGCTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTa > 1:3193112/1‑63 (MQ=255) tCGTGTTACAGGCGCTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTAc > 1:1484357/1‑64 (MQ=255) gtgtTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGGCAcgcc > 1:1965404/1‑71 (MQ=255) gtgtTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGGCAcgc > 1:864921/1‑70 (MQ=255) aCAGGCGTTTGACCCGCAGCCCGGCACGCTCTCGCCCGAGCGCaaa > 1:1582898/1‑46 (MQ=255) aCAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCaaa > 1:316716/1‑46 (MQ=255) aCAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGGCACGCCGctc < 1:2465646/70‑1 (MQ=255) aCAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGGCACGCCGct > 1:2201951/1‑69 (MQ=255) cGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTa > 1:4553216/1‑51 (MQ=255) cGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGGCACGCCGCTCTGCTac < 1:2838833/71‑1 (MQ=255) gTTTGACCGGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGGCACGCCGCTCTGCTaca > 1:4226990/1‑71 (MQ=255) | TACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGGCACGCCGCTCTGCTACA > W3110S.gb/286427‑286565 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |