| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,482,473 | A→G | 8.5% | L174P (CTC→CCC) | argI ← | ornithine carbamoyltransferase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,482,473 | 0 | A | G | 8.5% | 158.5 / 6.4 | 59 | L174P (CTC→CCC) | argI | ornithine carbamoyltransferase 1 |
| Reads supporting (aligned to +/- strand): ref base A (22/32); new base G (3/2); total (25/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.41e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.31e-01 | |||||||||||
AGCTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCC > W3110S.gb/4482406‑4482543 | aGCTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCa < 1:3456531/71‑1 (MQ=255) ccGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCg < 1:2628302/69‑1 (MQ=255) ccGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCg < 1:1940501/69‑1 (MQ=255) ccAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGc > 1:1350514/1‑71 (MQ=255) cAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGcc < 1:3491145/71‑1 (MQ=255) aGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGcc > 1:1699688/1‑70 (MQ=255) cACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGaa > 1:51090/1‑63 (MQ=255) aCGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCAtg > 1:4111527/1‑71 (MQ=255) aCGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCACAt < 1:3831163/70‑1 (MQ=255) cGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGaa > 1:2771012/1‑61 (MQ=255) cGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCAtgt > 1:2400721/1‑71 (MQ=255) cGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCAtgt > 1:2147171/1‑71 (MQ=255) ttGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAAt > 1:1176176/1‑59 (MQ=255) gtgGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCAtgttgt < 1:1331894/69‑1 (MQ=255) gtgGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTa > 1:575686/1‑71 (MQ=255) gcgACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGGGCATCGAATTGCCCATGTTGTTAcgcgc < 1:3945762/71‑1 (MQ=255) cgACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCAtgtt < 1:1798146/61‑1 (MQ=255) aCCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGGGCATCg < 1:1909094/46‑1 (MQ=255) aCCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTc < 1:895442/71‑1 (MQ=255) aCCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTc < 1:1222165/71‑1 (MQ=255) cAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGGGCATCGaa > 1:3204772/1‑46 (MQ=255) cAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGGGCATCGAATTGCCCATGTTGTTACGCGCGTCAc > 1:3247205/1‑71 (MQ=255) gCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGaa > 1:3473051/1‑41 (MQ=255) aaTCCAGACCGGTAAGCGCCGCAGCTTCGATCATCGAATTGCCCAtgtt < 1:4613067/49‑1 (MQ=255) aaTCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCAtgttgt < 1:2505319/51‑1 (MQ=255) aaTCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCAtgtt < 1:3969846/49‑1 (MQ=255) aaTCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCAtgtt < 1:4042076/49‑1 (MQ=255) aaTCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCAtgtt < 1:3607196/49‑1 (MQ=255) cAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCa > 1:1684648/1‑64 (MQ=255) gACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGt < 1:1791403/54‑1 (MQ=255) aCCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGAc > 1:4485414/1‑66 (MQ=255) ggTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCAtgttgt < 1:3067262/41‑1 (MQ=255) ggTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCa < 1:3884810/71‑1 (MQ=255) gTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCAt < 1:1189041/71‑1 (MQ=255) tAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTc < 1:200660/68‑1 (MQ=255) tAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCAtt > 1:1070178/1‑71 (MQ=255) aaGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTAcg > 1:3949009/1‑42 (MQ=255) aaGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCAttt > 1:4240886/1‑71 (MQ=255) aGCGCCGCAGCTTCGGGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCa > 1:3609974/1‑61 (MQ=255) aGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTc < 1:108090/71‑1 (MQ=255) gcgcCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTa < 1:2961969/38‑1 (MQ=255) ggccgcAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAg < 1:2358581/54‑1 (MQ=255) gccgcAGCTTCGAGCATCGAATTGCCCATGTTGTTa > 1:79301/1‑36 (MQ=255) gccgcAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGt > 1:2746133/1‑70 (MQ=255) ccgcAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGtt < 1:4389223/70‑1 (MQ=255) ccgcAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTg < 1:197923/71‑1 (MQ=255) cgcAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCa > 1:3338780/1‑49 (MQ=255) cgcAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGa < 1:1952843/71‑1 (MQ=255) gcAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACATGCATAGACCAGc > 1:1031516/1‑57 (MQ=255) cAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAAc > 1:326331/1‑71 (MQ=255) cAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAAc > 1:2266554/1‑71 (MQ=255) gCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCa < 1:1238811/45‑1 (MQ=255) gCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCa < 1:811566/45‑1 (MQ=255) cTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCAc < 1:60314/39‑1 (MQ=255) cTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCAt < 1:3484468/45‑1 (MQ=255) cTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTc < 1:754778/61‑1 (MQ=255) cTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGtt < 1:1843797/64‑1 (MQ=255) ttCGAGCATCGAATTGCCCATGTTGTTACGCGCGTc < 1:2027374/36‑1 (MQ=255) tCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGa > 1:1449967/1‑46 (MQ=255) aGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGcc < 1:1485247/71‑1 (MQ=255) | AGCTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCC > W3110S.gb/4482406‑4482543 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |