| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,525,729 | A→G | 6.7% | V325A (GTT→GCT) | yjhF ← | predicted transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,525,729 | 0 | A | G | 6.7% | 198.6 / 5.8 | 75 | V325A (GTT→GCT) | yjhF | predicted transporter |
| Reads supporting (aligned to +/- strand): ref base A (42/28); new base G (3/2); total (45/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.88e-01 | |||||||||||
AGGATCGGGTTAATATCCATACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGCAATACCCGCGATAGCTTTTCCGC > W3110S.gb/4525660‑4525798 | aGGATCGGGTTAATATCCATACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGaa > 1:1720283/1‑70 (MQ=255)aGGATCGGGTTAATATCCATACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAAc > 1:938270/1‑71 (MQ=255)aGGATCGGGTTAATATCCATACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAAc > 1:3612061/1‑71 (MQ=255)aGGATCGGGTTAATATCCATACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAAc > 1:4282890/1‑71 (MQ=255)aGGATCGGGTTAATATCCATACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAAc > 1:671255/1‑71 (MQ=255) cGGGTTAATATCCATACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGaa > 1:777247/1‑65 (MQ=255) cGGGTTAATATCCATACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAActtgct > 1:2352577/1‑71 (MQ=255) cGGGTTAATATCCATACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAActtgct > 1:2358035/1‑71 (MQ=255) gggTTAATATCCATACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGaa < 1:745725/64‑1 (MQ=255) gTTAATATCCATACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAGCTTGCTTGa < 1:3533185/71‑1 (MQ=255) tAATATCCATACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAACTTGCTTGaa > 1:776446/1‑70 (MQ=255) aaTATCCATACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATg > 1:326194/1‑71 (MQ=255) atatCCATACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAACTTGCTTGaa > 1:473963/1‑68 (MQ=255) atatCCATACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAACTTGCTTGaa > 1:2579108/1‑68 (MQ=255) ccATACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAACTTGCTTGaa > 1:3333936/1‑64 (MQ=255) cATACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGaa > 1:3080285/1‑53 (MQ=255) cATACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAAc > 1:1273645/1‑54 (MQ=255) cATACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAAc > 1:2921649/1‑54 (MQ=255) aTACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAGCTTGCTTGAATGcaccacc < 1:4016744/71‑1 (MQ=255) cccGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAAc > 1:1227596/1‑50 (MQ=255) cccGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAACTTGCTTGaa > 1:1267497/1‑59 (MQ=255) cccGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAACTTGCTTGaa > 1:3378986/1‑59 (MQ=255) cccGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGcaccaccac > 1:630905/1‑70 (MQ=255) ccGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAActt < 1:1292809/51‑1 (MQ=255) cGAAACCAGGGTGGGAATATACTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGcaccaccaccac > 1:1025737/1‑71 (MQ=255) cGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGcaccaccaccac > 1:2347935/1‑71 (MQ=255) aCCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAAc > 1:105044/1‑44 (MQ=255) ccAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATa < 1:1231135/71‑1 (MQ=255) cAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATa > 1:125385/1‑70 (MQ=255) aGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAAt > 1:1922738/1‑71 (MQ=255) gggTGGAAATATACTGACCAACGCCAGAGTCGATGAGAActtg > 1:3597447/1‑43 (MQ=255) gggTGGAAATATACTGACAAACGCCAGAGTCGATGAGAACTTGCTTGaa > 1:285031/1‑49 (MQ=255) gTGGAAATATACTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATcag > 1:4336985/1‑71 (MQ=255) gAAATATACTGACCAACGCCAGAGTCGATGAGAActt < 1:136303/37‑1 (MQ=255) atatACTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATcagcagca < 1:2677519/70‑1 (MQ=255) atatACTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATcagcagca < 1:3814796/70‑1 (MQ=255) tataCTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATcagcagcag < 1:399885/70‑1 (MQ=255) ataCTGACCAACGCCAGAGTCGATGAGAACTTGCTTGaa > 1:158142/1‑39 (MQ=255) ataCTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATcagcagca > 1:2023161/1‑68 (MQ=255) taCTGACCAACGCCAGAGTCGATGAGCACTTGCTTGaa > 1:1564555/1‑38 (MQ=255) taCTGACCAACGCCAGAGTCGATGAGAActtgcttg > 1:2491043/1‑36 (MQ=255) taCTGACCAACGCCAGAGTCGATGAGAActtgcttg < 1:1451550/36‑1 (MQ=255) taCTGACCAACGCCAGAGTCGATGAGAACTTGCTTGaa > 1:4047575/1‑38 (MQ=255) taCTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAAt > 1:4490504/1‑39 (MQ=255) taCTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGAcc > 1:287676/1‑71 (MQ=255) taCTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGAcc < 1:2126931/71‑1 (MQ=255) taCTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGAc > 1:1143584/1‑70 (MQ=255) aCTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATcagcag < 1:3019837/64‑1 (MQ=255) cTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCgg > 1:531794/1‑71 (MQ=255) tGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGcacc < 1:4555718/41‑1 (MQ=255) tGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATcagcagcag < 1:1935908/65‑1 (MQ=255) aCCAACGCCATAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGc < 1:3033819/69‑1 (MQ=255) aCCAACGCCAGAGTCGATGAGAGCTTGCTTGAATGCACCACCACCACCGATaa > 1:2916088/1‑53 (MQ=255) aCCAACGCCAGAGTCGATGAGAACTTGCTTGAATGca > 1:1447435/1‑37 (MQ=255) aCCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATcagcagcag > 1:2065858/1‑63 (MQ=255) aCCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGAc > 1:159404/1‑65 (MQ=255) aCCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGc < 1:2756808/69‑1 (MQ=255) aCCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGc < 1:2751558/69‑1 (MQ=255) aCCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGCaa < 1:110632/71‑1 (MQ=255) cAACGCCAGAGTCGATGAGAGCTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGCAATa > 1:3671823/1‑71 (MQ=255) cAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATcagcag < 1:3548151/58‑1 (MQ=255) aaCGCCAGAGTCGATGAGAGCTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGCAATAc > 1:1641429/1‑71 (MQ=255) aCGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGCAATAcc < 1:2462636/71‑1 (MQ=255) cGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATcagcag < 1:3297906/55‑1 (MQ=255) gCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATcag > 1:3360119/1‑51 (MQ=255) cAGAGTCGATGAGAACTTGCTTGAATGcaccaccac < 1:1534825/36‑1 (MQ=255) cAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGCAATACCcgcg < 1:396792/71‑1 (MQ=255) tCGATGAGAACTTGCTTGAATGCACCACCACCTCCGATa < 1:593602/39‑1 (MQ=255) tCGATGAGAACTTGCTTGAATGCACCACCACCACCGa < 1:2185488/37‑1 (MQ=255) tCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGCAATACCCGCGATAGc > 1:3638308/1‑71 (MQ=255) gATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGCAATACCCGCGATAGCtt > 1:1705608/1‑71 (MQ=255) aTGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGCaa < 1:1977872/55‑1 (MQ=255) gagaACTTGCTTGAATTCACCACCACCACCGATAATCAGCAGCAGACCGGc < 1:2846508/51‑1 (MQ=255) gagaACTTGCTTGAATGCACCACCACCACCGATAATcag > 1:3161392/1‑39 (MQ=255) gagaACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGc < 1:498289/51‑1 (MQ=255) gagaACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGc < 1:2759410/51‑1 (MQ=255) gagaACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGc < 1:1698374/51‑1 (MQ=255) gagaACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGc < 1:2049581/51‑1 (MQ=255) gagaACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGCAATACCCGCGATAGCTTTTc > 1:2758708/1‑71 (MQ=255) aaCTTGCTTGAATGCACCACCACCACCGATAATcagcagca > 1:4552265/1‑41 (MQ=255) aaCTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGCAATACCCGCGATAGCTTTTCCGc < 1:2012051/71‑1 (MQ=255) | AGGATCGGGTTAATATCCATACCCGAAACCAGGGTGGAAATATACTGACCAACGCCAGAGTCGATGAGAACTTGCTTGAATGCACCACCACCACCGATAATCAGCAGCAGACCGGCAATACCCGCGATAGCTTTTCCGC > W3110S.gb/4525660‑4525798 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |