| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,526,495 | T→C | 12.9% | M70V (ATG→GTG) | yjhF ← | predicted transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,526,495 | 0 | T | C | 12.9% | 158.0 / 15.5 | 62 | M70V (ATG→GTG) | yjhF | predicted transporter |
| Reads supporting (aligned to +/- strand): ref base T (31/23); new base C (5/3); total (36/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
CCGAAATAATTCAGCATGGTCAGCGCTATTCGCTGCGCACCACCCGCATCGGCCAGCAAGCGACCGAGCATAACACCGAAGCCAAATATCAAACCAATATGGCCCAGCGTACCGCCAAGTCCGGATTCGACTGAGGTGAC > W3110S.gb/4526425‑4526564 | ccGAAATAATTCAGCATGGTCAGCGCTATTCGCTGCGCACCACCCGCATCGGCCAGCAAGCGACCGAGCAt < 1:3601402/71‑1 (MQ=255) gAAATAATTCAGCATGGTCAGCGCTATTCGCTGCGCACCACCCGCATCGGCCAGCAAGCGACCGAGCATaa > 1:135042/1‑71 (MQ=255) ataatTCAGCATGGTCAGCGCTATTCGCTGCGCACCACCCGCATCGGCCAGCAAGCGACCGAGCATAacac > 1:37467/1‑71 (MQ=255) taatTCAGCATGGTCAGCGCTATTCGCTGCGCACCACCCGCATCGGCCAGCAAGCGACCGAGCATAACAcc < 1:3817296/71‑1 (MQ=255) gCATGGTCAGCGCTATTCGCTGCGCACCACCCGCATCGGCCAGCAAGCGACCGAGCATAACACCGAAGCCa < 1:4595878/71‑1 (MQ=255) aTGGTCAGCGCTATTCGCTGCGCACCACCCGCATCGGCCAGCAAGCGACCGAGCATAACACCGAAGCCaaa < 1:2970111/71‑1 (MQ=255) aTGGTCAGCGCTATTCGCTGCGCACCACCCGCATCGGCCAGCAAGCGACCGAGCATAACACCGAAGCCaaa < 1:4200379/71‑1 (MQ=255) tCAGCGCTATTCGCTGCGCACCACCCGCATCGGCCAGCAAGCGACCGAGCATAACACCGAAGCCAAatat > 1:3457055/1‑70 (MQ=255) aGCGCTATTCGCTGCGCACCACCCGCATCGGCCAGCAAGCGACCGAGCATAACACCGAAGCCAAATATCaa < 1:1181027/71‑1 (MQ=255) aGCGCTATTCGCTGCGCACCACCCGCATCGGCCAGCAAGCGACCGAGCATAACACCGAAGCCAAATATCa > 1:2197773/1‑70 (MQ=255) cgcTATTCGCTGCGCACCACCCGCATCGGCCAGCAAGCGACCGAGCATAACACCGAAGCCa < 1:1268506/61‑1 (MQ=255) cgcTATTCGCTGCGCACCACCCGCATCGGCCAGCAAGCGACCGAGCATAACACCGAAGCCAAATAACAAAc > 1:3733546/1‑71 (MQ=255) gcTATTCGCTGCGCACCACCCGCATCGGCCAGCAAGCGACCGAGCATAACACCGAAGCCAAATATCAAAcc < 1:245188/71‑1 (MQ=255) cTATTCGCTGCGCACCACCCGCATCGGCCAGCAAGCGACCGAGCATAACACCGAAGCCAAatat > 1:3997924/1‑64 (MQ=255) tATTCGCTGCGCACCACCCGCATCGGCCAGCAAGCGACCGAGCATAACACCGAAGCCa < 1:1856986/58‑1 (MQ=255) aTTCGCTGCGCACCACCCGCATCGGCCAGCAAGCGACCGAGCATAACACCGAAGCCAAATATCAAACCaa > 1:4075835/1‑70 (MQ=255) aTTCGCTGCGCACCACCCGCATCGGCCAGCAAGCGACCGAGCATAACACCGAAGCCAAATATCAAACCaa > 1:2189080/1‑70 (MQ=255) ttCGCTGCGCACCACCCGCATCGGCCAGCAAGCGACCGAGCATaa > 1:1107847/1‑45 (MQ=255) ttCGCTGCGCACCACCCGCATCGGCCAGCAAGCGACCGAGCATAACACCGAAGCCAAATATCAAACCAata > 1:2073974/1‑71 (MQ=255) ttCGCTGCGCACCACCCGCATCGGCCAGCAAGCGACCGAGCACAACACCGAAGCCAAATATCAAACCaa < 1:4430597/69‑1 (MQ=255) ttCGCTGCGCACCACCCGCATCGGCCAGCAAGCGACCGAGCACAACACCGAAGCCAAATATCAAACCAata > 1:2012660/1‑71 (MQ=255) cGCTGCGCACCACCCGCATCGGCCAGCAAGCGACCGAGCATAACACCGAAGCCAAATATCAAACCAata > 1:452165/1‑69 (MQ=255) cGCTGCGCACCACCCGCATCGGCCAGCAAGCGACCGAGCATAACACCGAAGCCAAATATCAAACCAATATg > 1:3872718/1‑71 (MQ=255) ccaccCGCATCGGCCAGCAAGCGACCGAGCATAACACCGAAGCCAAATATCAAAcc < 1:1348787/56‑1 (MQ=255) caccCGCATCGGCCAGCAAGCGACCGAGCACAACACCGAAGCCAAATATCAAACCAATATGGCCCAGCGTa < 1:4191474/71‑1 (MQ=255) accCGCATCGGCCAGCAAGCGACCGAGCATAACACCGAAGCCAAATATCAAACCaa > 1:264432/1‑56 (MQ=255) accCGCATCGGCCAGCAAGCGACCGAGCATAACACCGAAGCCAAATATCAAACCAATATgg > 1:1275817/1‑61 (MQ=255) ccGCATCGGCCAGCAAGCGACCGAGCATAACACCGAAGCCAAatat < 1:3275726/46‑1 (MQ=255) cGCATCGGCCAGCAAGCGACCGAGCATAACACCGAAGCCAAATATCAAACCaa > 1:620740/1‑53 (MQ=255) gCATCGGCCAGCAAGCGACCGAGCATAACACCGAAGCCaaa > 1:222019/1‑41 (MQ=255) gCATCGGCCAGCAAGCGACCGAGCATAACACCGAAGCCAAATATCAAACCAATATGGCCCAGCGTACCGc > 1:2882465/1‑70 (MQ=255) aTCGGCCAGCAAGCGACCGAGCACAACACCGAAGCCAAATATCAAACCaa < 1:4295961/50‑1 (MQ=255) tCGGCCAGCAAGCGACCGAGCATAACACCGAAGCCAAATATCAAAc < 1:2506942/46‑1 (MQ=255) ggCCAGCAAGCGACCGAGCATAACACCGAAGCCAAATATCAAACCAata > 1:4548632/1‑49 (MQ=255) ggCCAGCAAGCGACCGAGCATAACACCGAAGCCAAATATCAAACCAat > 1:2368344/1‑48 (MQ=255) ggCCAGCAAGCGACCGAGCATAACACCGAAGCCAAATATCAAACCAATATGGCCCAGCGTACCGCCAAGTc > 1:3266640/1‑71 (MQ=255) ggCCAGCAAGCGACCGAGCATAACACCGAAGCCAAATATCAAACCAATATGGCCCAGCGTACCGCCAAGTc > 1:512554/1‑71 (MQ=255) ggCCAGCAAGCGACCGAGCATAACACCGAAGCCAAATATCAAACCAATATGGCCCAGCGTACCGCCAAGTc > 1:3794715/1‑71 (MQ=255) ggCCAGCAAGCGACCGAGCACAACACCGAAGCCAAATATCAAACCAATATGGCCCAGCGTACCGCCAAGTc > 1:2244344/1‑71 (MQ=255) ggCCAGCAAGCGACCGAGCACAACACCGAAGCCAAATATCAAACCAATATGGCCCAGCGTACCGCCAAGTc > 1:3785260/1‑71 (MQ=255) ccAGCAAGCGACCGAGCATAACACCGAAGCCAAATATCAAACCAATATGGCCCAGCGTACCGCCAAGTCCg > 1:3044239/1‑71 (MQ=255) ccAGCAAGCGACCGAGCATAACACCGAAGCCAAATATCAAACCAATATGGCCCAGCGTACCGCCAAGTCCg < 1:2762853/71‑1 (MQ=255) cAGCAAGCGACCGAGCACAACACCGAAGCCAAATATCAAACCAATATGGCCCAGCGTACCGCCAAGTCCg > 1:2439148/1‑70 (MQ=255) aGCAAGCGACCGAGCATAACACCGAAGCCAAATATCAAACCAATATGGCCCAGCGTACCGCCAAGTCCgca < 1:3459172/71‑3 (MQ=255) cAAGCGACCGAGCATAACACCGAAGCCAAATATCAAACCAATATGGCCCAGCGTACCGCCAAGTCCGGAtt > 1:2216215/1‑71 (MQ=255) cAAGCGACAGAGCATAACACCGAAGCCAAATATCAAACCAATATGGCCCAGCGt < 1:1478290/54‑1 (MQ=255) aaGCGACCGAGCATAACACCGAAGCCAAATATCAAACCAATATGGCCCAGCGTACCGCCAAGTCCGGATTc > 1:1144977/1‑71 (MQ=255) aaGCGACCGAGCATAACACCGAAGCCAAATATCAAACCAATATGGCCCAGCGTACCGCCAAGTCCGGATTc < 1:3699725/71‑1 (MQ=255) gCGACCGAGCATAACACCGAAGCCAAATATCAAACCAATATGGCCCAGCGTACCGCCAAGTCCGGATTCGa > 1:1474551/1‑71 (MQ=255) gACCGAGCATAACACCGAAGCCAAATATCAAACCAATATGGCCCAGCGTACCGCCAAGTCCGGATTCGACt > 1:3826562/1‑71 (MQ=255) gACCGAGCACAACACCGAAGCCAAATATCAAACCAATATGGCCCAGCGTACCGCCAAGTCCGGa > 1:1873227/1‑64 (MQ=255) aCCGAGCATAACACCGAAGCCAAATATCAAACCAATATGGCCCAGCGTACCGCCAAGTCCGGATTCGACTg < 1:4564670/71‑1 (MQ=255) ccGAGCATAACACCGAAGCCAAATATCAAACCAATATGGCCCAGCg < 1:2732015/46‑1 (MQ=255) ccGAGCATAACACCGAAGCCAAATATCAAACCAATATGGCCCAGCg < 1:1292266/46‑1 (MQ=255) ccGAGCATAACACCGAAGCCAAATATCAAACCAATATGGCCCAGCGTACCGCCaa > 1:3400077/1‑55 (MQ=255) gAGCATAACACCGAAGCCAAATATCAAACCAATATGGCCCAGCGTACCGCCAAGTCCGGATTCGACTGAg > 1:1201387/1‑70 (MQ=255) aGCATAACACCGAAGCCAAATATCAAACCAATATGGCCCAg < 1:804091/41‑1 (MQ=255) gCATAACACCGAAGCCAAATATCAAACCAATATGGCCCAGCGTACCGCCAAGTCCGGATTCGACTGAGGt > 1:4045110/1‑70 (MQ=255) gCATAACACCGAAGCCAAATATCAAACCAATATGGCCCAGCGTACCGCCAAGTCCGGATTCGACTGAGGTg < 1:3194584/71‑1 (MQ=255) gCATAACACCGAAGCCAAATATCAAACCAATATGGCCCAGCGTACCGCCAAGTCCGGATTCGACTGAGGTg < 1:2875534/71‑1 (MQ=255) cATAACACCGAAGCCAAATATCAAACCAATATGGc > 1:1287086/1‑35 (MQ=255) aTAACACCGAAGCCAAATATCAAACCAATATGGCCCAGCg < 1:3004494/40‑1 (MQ=255) aTAACACCGAAGCCAAATATCAAACCAATATGGCCCAGCg < 1:671136/40‑1 (MQ=255) tAACACCGAAGCCAAATATCAAACCAATATGGCCCAGCGTACCGCCAAGTCCGGATTCg < 1:2317658/59‑1 (MQ=255) tAACACCGAAGCCAAATATCAAACCAATATGGCCCAGCGTACCGCCAAGTCCGGATTCGACTGAGGTgac > 1:4472988/1‑70 (MQ=255) tAACACCGAAGCCAAATATCAAACCAATATGGCCCAGCGTACCGCCAAGTCCGGATTCGACTGAGGTgac > 1:1364855/1‑70 (MQ=255) | CCGAAATAATTCAGCATGGTCAGCGCTATTCGCTGCGCACCACCCGCATCGGCCAGCAAGCGACCGAGCATAACACCGAAGCCAAATATCAAACCAATATGGCCCAGCGTACCGCCAAGTCCGGATTCGACTGAGGTGAC > W3110S.gb/4526425‑4526564 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |