| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,546,724 | T→C | 30.7% | S2S (AGT→AGC) | fimE → | tyrosine recombinase/inversion of on/off regulator of fimA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,546,724 | 0 | T | C | 30.7% | 97.8 / 64.0 | 75 | S2S (AGT→AGC) | fimE | tyrosine recombinase/inversion of on/off regulator of fimA |
| Reads supporting (aligned to +/- strand): ref base T (24/28); new base C (15/8); total (39/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.28e-01 | |||||||||||
TTGACCGATTGAGGTTTCCTATAGGTATTCATTCAAATATATCTCAGTTAGGAGTACTACTATTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCAGGCGGTTTGTTACGGGGCAACGG > W3110S.gb/4546655‑4546791 | ttGACCGATTGAGGTTTCCTATAGGTATTCATTCAAATATATCTCAGTTAGGAGTACTACTATTGTGAGt > 1:1466412/1‑70 (MQ=255) gACCGATTGAGGTTTCCTATAGGTATTCATTCAAATATATCTCAGTTAGGAGTACTACTATTGTGAGTaa < 1:2116439/70‑1 (MQ=255) aCCGATTGAGGTTTCCTATAGGTATTCATTCAAATATATCTCAGTTAGGAGTACTACTATTGTGAGTAAAc < 1:2431385/71‑1 (MQ=255) gATTGAGGTTTCCTATAGGTATTCATTCAAATATATCTCAGTTAGGAGTACTACTATTGTGAGTAAAcgtc < 1:2264432/71‑1 (MQ=255) ttGAGGTTTCCTATAGGTATTCATTCAAATATATCTCAGTTAGGAGTACTACTATTGTGAGCAAAcgtcgt < 1:1051760/71‑1 (MQ=255) tGAGGTTTCCTATAGGTATTCATTCAAATATATCTCAGTTAGGAGTACTACTATTGTGAGTAAAcgtcgt > 1:2976426/1‑70 (MQ=255) gTTTCCTATAGGTATTCATTCAAATATATCTCAGTTAGGAGTACTACTATTGTGAGTAAACGTCGtt < 1:2293784/67‑1 (MQ=255) tttCCTATAGGTATTCATTCAAATATATATCAGTTAGGAGTACTACTATTGTGAGTaa < 1:3090204/58‑1 (MQ=255) tCCTATAGGTATTCATTCAAATATATCTCAGTTAGGAGTACTACTATTGTGAGTAAAc > 1:717630/1‑58 (MQ=255) tCCTATAGGTATTCATTCAAATATATCTCAGTTAGGAGTACTACTATTGTGAGTAAACGTCGTTATCTTAc > 1:3905287/1‑71 (MQ=255) ccTATAGGTATTCATTCAAATATATCTCAGTTAGGAGTACTACTATTGTGAGTAAACGTCGTTATCTTAcc > 1:1840481/1‑71 (MQ=255) ggTATTCATTCAAATATATCTCAGTTAGGAGTACTACTATTGTGAGCAAACGTCGTTATCTTACCGGTaa < 1:3800068/70‑1 (MQ=255) gTATTCATTCAAATATATCTCAGTTAGGAGTACTACTATTGTGAGTAAACGTCGTTATCTTACCGGTAaag < 1:1946369/71‑1 (MQ=255) gTATTCATTCAAATATATCTCAGTTAGGAGTACTACTATTGTGAGCAAACGTCGTTATCTTACCGGTa > 1:402791/1‑68 (MQ=255) attcattcAAATATATCTCAGTTAGGAGTACTACTATTGTGAGCAAACGTCGTTATCTTACCGGTAaag > 1:821632/1‑69 (MQ=255) ttcattcaAATATATCTCAGTTAGGAGTACTACTATTGTGAGTa < 1:1108336/44‑1 (MQ=255) ttcattcaAATATATCTCAGTTAGGAGTACTACTATTGTGAGTAAACGTCGTTATCTTACCGGTAaagaag < 1:4102094/71‑1 (MQ=255) ttcattcaAATATATCTCAGTTAGGAGTACTACTATTGTGAGTAAACGTCGTTATCTTACCGGTAaagaa < 1:129869/70‑1 (MQ=255) ttcattcaAATATATCTCAGTTAGGAGTACTACTATTGTGAGTAAACGTCGTTATCTTACCGGTAaag < 1:2275420/68‑1 (MQ=255) tcattcaAATATATCTCAGTTAGGAGTACTACTATTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGt > 1:3454365/1‑71 (MQ=255) cattcaAATATATCTCAGTTAGGAGTACTACTATTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGtt > 1:2513201/1‑71 (MQ=255) cattcaAATATATCTCAGTTAGGAGTACTACTATTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGtt > 1:4443013/1‑71 (MQ=255) attcaAATATATCTCAGTTAGGAGTACTACTATTGTGAGCAAACGTCGTTATCTTACCGGTAAAGAAGtt < 1:1279600/70‑1 (MQ=255) attcaAATATATCTCAGTTAGGAGTACTACTATTGTGAGCAAACGTCGTTATCTTACCGGTAAAGAAGtt > 1:26040/1‑70 (MQ=255) tcaAATATATCTCAGTTAGGAGTACTACTATTGTGAGTAAACGTCGTTAt > 1:3975110/1‑50 (MQ=255) tcaAATATATCTCAGTTAGGAGTACTACTATTGTGAGTAAACGTCGTTAt > 1:2813582/1‑50 (MQ=255) caAATATATCTCAGTTAGGAGTACTACTATTGTGAGTaaa > 1:1790192/1‑40 (MQ=255) caAATATATCTCAGTTAGGAGTACTACTATTGTGAGTAAACGTCGTTATCTTACCGGTaaa < 1:518867/61‑1 (MQ=255) caAATATATCTCAGTTAGGAGTACTACTATTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAgg > 1:3330638/1‑71 (MQ=255) atatatCTCAGTTAGGAGTACTACTATTGTGAGTAAACGTCGTTATcct < 1:1602576/49‑3 (MQ=255) tatatCTCAGTTAGGAGTACTACTATTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCa > 1:2882627/1‑65 (MQ=255) tatCTCAGTTAGGAGTACTACTATTGTGAGTAAACGTCGTTATCTTa > 1:1987250/1‑47 (MQ=255) aGTTAGGAGTACTACTATTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCAgg > 1:1831422/1‑71 (MQ=255) aGTTAGGAGTACTACTATTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCAg > 1:4306987/1‑70 (MQ=255) aGTTAGGAGTACTACTATTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGATCAGGCCATGATGCAgg > 1:2905713/1‑71 (MQ=255) ttAGGAGTACTACTATTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCa < 1:2707776/67‑1 (MQ=255) aGGAGTACTACTATTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCatga > 1:980351/1‑61 (MQ=255) gTACTACTATTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCAGGCGGTTTGt < 1:2977107/71‑1 (MQ=255) gTACTACTATTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCAGGCGGTTTGt < 1:1620997/71‑1 (MQ=255) tactacTATTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGcc < 1:3237842/52‑1 (MQ=255) tactacTATTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCat < 1:464896/54‑1 (MQ=255) ctactaTTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCatga > 1:309953/1‑54 (MQ=255) ctactaTTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCatga > 1:2449250/1‑54 (MQ=255) ctactaTTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCAGGCGGttt > 1:1064023/1‑66 (MQ=255) tactaTTGTGAGTAAACGTCGTTATCTTACCGGTaaa < 1:3206481/37‑1 (MQ=255) tactaTTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCa > 1:3974116/1‑45 (MQ=255) tactaTTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCat < 1:139136/51‑1 (MQ=255) tactaTTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCAGGCGGt < 1:320044/63‑1 (MQ=255) actaTTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGtt > 1:848564/1‑42 (MQ=255) actaTTGTGAGCAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCat < 1:2354773/50‑1 (MQ=255) actaTTGTGAGCAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCat < 1:3470281/50‑1 (MQ=255) ctaTTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCAGGCGGTTTGTTACggg > 1:2943384/1‑71 (MQ=255) taTTGTGAGTAAACGTCGTTATCTTACCGGTAaagaag < 1:1290117/38‑1 (MQ=255) ttGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCAGGCGGTTTGTTACGGGGc < 1:373226/70‑1 (MQ=255) ttGTGAGCAAACGTCGTTATCTTACCGGTAAAGAAGtta > 1:1626623/1‑38 (MQ=39) ttGTGAGCAAACGTCGTTATCTTACCGGTAAAGAAGTTc > 1:4383289/1‑39 (MQ=255) ttGTGAGCAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCatgat > 1:326392/1‑49 (MQ=255) ttGTGAGCAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCAGGCGGTTTGTTACg > 1:3275023/1‑66 (MQ=255) tgtgAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCAGGCGGTTTGTTa < 1:2327693/63‑1 (MQ=255) gtgAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCat < 1:4084255/44‑1 (MQ=255) gtgAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCAGGCGGTTTGTTACGGGGCAAc > 1:2464716/1‑71 (MQ=255) gtgAGCAAACGTCGTTATCTTACCGGTAAAGAAGTTc > 1:2915761/1‑37 (MQ=255) gtgAGCAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCa > 1:4494429/1‑43 (MQ=255) gtgAGCAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCa > 1:89461/1‑43 (MQ=255) gtgAGCAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGc > 1:2615352/1‑49 (MQ=255) gtgAGCAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGc > 1:3043820/1‑49 (MQ=255) gtgAGCAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCAGGCGGTTTGtt > 1:3752474/1‑61 (MQ=255) gtgAGCAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCAGGCGGTTTGTTACGGGGCAAc > 1:935751/1‑71 (MQ=255) tgAGCAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCAGGCGGttt < 1:1571710/57‑1 (MQ=255) tgAGCAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCAGGCGGTTTGTTa < 1:3133920/61‑1 (MQ=255) tgAGCAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCAGGCGGTTTGTTACGGGGCAAc < 1:34409/70‑1 (MQ=255) gAGCAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCAGGCGGTTTGTTACGGGGCAACgg > 1:4135767/1‑71 (MQ=255) aGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAg < 1:4287001/36‑1 (MQ=255) aGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAg < 1:3132888/36‑1 (MQ=255) aGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCAGGCGGTTTGTTACGGGGCAACgg < 1:2910228/70‑1 (MQ=255) gTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCAGGCGGTTTGTTACGGGGCAACgg < 1:1927527/69‑1 (MQ=255) tAAACGTCGTTATCTTACCGGTAAAGGAGTTCAGGCCATGATGCAGGCGGTTTGTTACgg > 1:3608977/1‑60 (MQ=255) tAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCAGGCGGt < 1:1143621/51‑1 (MQ=255) | TTGACCGATTGAGGTTTCCTATAGGTATTCATTCAAATATATCTCAGTTAGGAGTACTACTATTGTGAGTAAACGTCGTTATCTTACCGGTAAAGAAGTTCAGGCCATGATGCAGGCGGTTTGTTACGGGGCAACGG > W3110S.gb/4546655‑4546791 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |