| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,563,589 | T→A | 100% | K207M (AAG→ATG) | iadA ← | isoaspartyl dipeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,563,589 | 0 | T | A | 100.0% | 146.5 / NA | 51 | K207M (AAG→ATG) | iadA | isoaspartyl dipeptidase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (33/18); total (33/18) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGG > W3110S.gb/4563523‑4563654 | gTCGGCAGCAGGTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCatttt < 1:2419310/71‑1 (MQ=255)gTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCatttt < 1:2696401/71‑1 (MQ=255) ggCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACt < 1:547474/71‑1 (MQ=255) ggCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGAAACGCCATTTTACt > 1:4099933/1‑71 (MQ=255) ttGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAAGGCCATTTTACTGTCGCCCAt > 1:641148/1‑71 (MQ=255) ttGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGccc < 1:770635/69‑1 (MQ=255) gCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAt < 1:4527225/69‑1 (MQ=255) cTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtgtg < 1:652548/71‑1 (MQ=255) tGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACt > 1:3358199/1‑58 (MQ=255) tGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACt > 1:3552578/1‑58 (MQ=255) tGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACt > 1:2815138/1‑58 (MQ=255) tGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACt > 1:249295/1‑58 (MQ=255) tGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACt > 1:631765/1‑58 (MQ=255) tGATCGGCACATCGCAGTTTTCAAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtgtg > 1:3076/1‑70 (MQ=255) aTCGGCACATCGGAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTAGTGTCGGCCATGTgg < 1:233460/69‑1 (MQ=255) aTCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGaa > 1:4489704/1‑71 (MQ=255) aTCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGaa > 1:1514928/1‑71 (MQ=255) cGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtgtg > 1:679904/1‑66 (MQ=255) ggCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAaca < 1:2511349/70‑1 (MQ=255) aTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTg > 1:4578573/1‑70 (MQ=255) gCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGt > 1:2612106/1‑47 (MQ=255) gCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc > 1:1015876/1‑71 (MQ=255) cAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGcc < 1:2377153/71‑1 (MQ=255) gTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAg < 1:3513076/71‑1 (MQ=255) ttttCCAGCAGGTCATAAATAGGCTGTAACGCCatttt < 1:3608906/38‑1 (MQ=255) ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTa > 1:1481043/1‑37 (MQ=255) ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTAc > 1:4452210/1‑38 (MQ=255) ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTAc > 1:1089566/1‑38 (MQ=255) ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTgg < 1:882591/52‑1 (MQ=255) ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGtt > 1:582772/1‑71 (MQ=255) tCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGtt < 1:2462634/70‑1 (MQ=255) agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgcc < 1:344153/71‑1 (MQ=255) gTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTgg < 1:1662463/43‑1 (MQ=255) tCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAg < 1:4264392/71‑1 (MQ=255) tAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACg < 1:2848955/45‑1 (MQ=255) tAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCaa < 1:1280990/71‑1 (MQ=255) aaaTAGGCTGTAACTCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACC‑CCGAGCaaa > 1:2443218/1‑70 (MQ=255) aaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGaa > 1:3111758/1‑39 (MQ=255) aaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAacac < 1:53801/42‑1 (MQ=255) aaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAAc > 1:1736978/1‑71 (MQ=255) gCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACg < 1:2694362/38‑1 (MQ=255) tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAAc > 1:690679/1‑64 (MQ=255) gTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCa < 1:3552112/60‑1 (MQ=255) aaCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAAcgcg > 1:4090901/1‑71 (MQ=255) aaCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAAcgcg > 1:1814469/1‑71 (MQ=255) aaCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAAcgcg > 1:2937517/1‑71 (MQ=255) aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgcc > 1:192545/1‑52 (MQ=255) aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgcc > 1:2313128/1‑52 (MQ=255) aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgcc > 1:3396041/1‑52 (MQ=255) aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAc > 1:225543/1‑48 (MQ=255) aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCgg > 1:4168850/1‑71 (MQ=255) aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCgg > 1:3227959/1‑71 (MQ=255) gCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGa > 1:839923/1‑52 (MQ=255) gCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGa > 1:1092235/1‑52 (MQ=255) | GTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGG > W3110S.gb/4563523‑4563654 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |