| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,616,051 | T→C | 12.9% | intergenic (+366/‑27) | prfC → / → osmY | peptide chain release factor RF‑3/periplasmic protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,616,051 | 0 | T | C | 12.9% | 218.3 / 23.0 | 85 | intergenic (+366/‑27) | prfC/osmY | peptide chain release factor RF‑3/periplasmic protein |
| Reads supporting (aligned to +/- strand): ref base T (35/39); new base C (6/5); total (41/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.52e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01 | |||||||||||
TTGACTTCTGTAGAGGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTGAAGATTTCGAAAACTCTGCTGGCT > W3110S.gb/4615984‑4616119 | ttGACTTCTGTAGAGGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACg < 1:3099390/71‑1 (MQ=255) tGACTTCTGTAGAGGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACg > 1:3487715/1‑70 (MQ=255) gACTTCTGTAGAGGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGa < 1:2587857/70‑1 (MQ=255) gACTTCTGTAGAGGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAg > 1:6881/1‑71 (MQ=255) cTGTAGAGGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCaaa < 1:3043867/70‑1 (MQ=255) cTGTAGAGGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCaaa < 1:875691/70‑1 (MQ=255) cTGTAGAGGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCaaa < 1:1029892/70‑1 (MQ=255) cTGTAGAGGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCaaa < 1:1983294/70‑1 (MQ=255) tAGAGGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGACCAAAc < 1:1772048/68‑1 (MQ=255) agGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAg < 1:488360/71‑1 (MQ=255) gAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAAt > 1:3877956/1‑70 (MQ=255) aaGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATCACGAGCAAACAt > 1:2761068/1‑61 (MQ=255) aGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAgg > 1:3458926/1‑65 (MQ=255) tctctcCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATa < 1:940250/67‑1 (MQ=255) tctctcCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATa < 1:146264/67‑1 (MQ=255) tctctcCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATc < 1:3948955/71‑1 (MQ=255) tctctcCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATc < 1:3271574/71‑1 (MQ=255) tctctcCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATCACGAGCAAACATACAGGAATAAATc < 1:1161876/71‑1 (MQ=255) tctcCCTATTCAGCAATGCAACCTCGTGTTGCTAGGCTCAAATTACGAGCAAACATAc > 1:4033585/1‑58 (MQ=255) tctcCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACg > 1:2893110/1‑47 (MQ=255) tctcCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAAt > 1:832508/1‑68 (MQ=255) ctcCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGAt > 1:3542997/1‑71 (MQ=255) ctcCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGAt > 1:3973937/1‑71 (MQ=255) tcCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCg < 1:2147908/68‑1 (MQ=255) tcCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGAt > 1:4503222/1‑70 (MQ=255) ccTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATCACGAGCAAACATACAGGAATAAATCGAt < 1:4519484/68‑1 (MQ=255) ttCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTa > 1:1806029/1‑69 (MQ=255) ttCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTAt < 1:1075973/70‑1 (MQ=255) ttCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTAt > 1:2434318/1‑70 (MQ=255) cAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAAc > 1:41687/1‑44 (MQ=255) cAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGa > 1:576117/1‑70 (MQ=255) cAGCAATGCAACCTCGTGTTGCCAGGCTCAAATCACGAGCAAACATACAGGAATAAATCGATGACTATGa > 1:3792379/1‑70 (MQ=255) cAGCAATGCAACCTCGTGTTGCCAGGCTCAAATCACGAGCAAACATACAGGAATAAATCGATGACTATGa > 1:3338236/1‑70 (MQ=255) aGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATa < 1:1348560/46‑1 (MQ=255) aGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGAc > 1:2108506/1‑70 (MQ=255) aGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACa < 1:4445710/71‑1 (MQ=255) aGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACa < 1:4364124/71‑1 (MQ=255) cAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCaaa > 1:1099376/1‑40 (MQ=255) cAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCaaa > 1:4033772/1‑40 (MQ=255) cAATGCAACCTCGTGTTGCCAGGCTCAAATCACGAGCAAACATACAGGAATAAATCGATGACTATGACAAg < 1:529126/71‑1 (MQ=255) cAATGCAACCTCGTGTTGCCAGGCTCAAATCACGAGCAAACATACAGGAATAAATCGATGACTATGACAAg < 1:173045/71‑1 (MQ=255) aaTGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACAt > 1:1712350/1‑42 (MQ=255) aaTGCAACCTCGTGTTGCCAGGCTCAAATCACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGa > 1:1791047/1‑71 (MQ=255) tGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATa < 1:2781909/41‑1 (MQ=255) tGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGAt < 1:1385227/56‑1 (MQ=255) tGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGAt < 1:3705902/56‑1 (MQ=255) tGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGAt < 1:3700759/56‑1 (MQ=255) gCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATa > 1:3820437/1‑40 (MQ=255) gCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGa > 1:2209563/1‑54 (MQ=255) gCAACCACGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGa > 1:152789/1‑68 (MQ=255) cAACCTCGTGTTGCCAGGCTCAAATTACGAGCaaa > 1:2134890/1‑35 (MQ=255) cAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGa > 1:3110529/1‑53 (MQ=255) cAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGAt < 1:2112536/54‑1 (MQ=255) cAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGa > 1:854530/1‑56 (MQ=255) cAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGa > 1:712004/1‑56 (MQ=255) cAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTg < 1:397545/70‑1 (MQ=255) cAACCTCGTGTTGCCAGGCTCAAATCACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTg > 1:1639391/1‑70 (MQ=255) aaCCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTGaa < 1:228810/71‑1 (MQ=255) aCCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACaa > 1:1356334/1‑63 (MQ=255) aCCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTg > 1:3310742/1‑68 (MQ=255) ccTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAgg < 1:780693/40‑1 (MQ=255) ccTCGTGTTGCCAGGCTCAAATCACGAGCAAACATACAGGAATa < 1:3272644/44‑1 (MQ=255) cGTGTTGCCAGGCTCAATTTACGAGCAAACATACAGGAATAAATCGATGACTATGACaa < 1:4633521/59‑1 (MQ=255) gtgtTGCCAGGCTCAAATCACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTGAAGATTTc > 1:2476370/1‑71 (MQ=255) tgtTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTGAAGATTTCg < 1:3816522/71‑1 (MQ=255) ttGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTGaa < 1:606061/62‑1 (MQ=255) ttGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTGAAGATTTCGaa < 1:2811000/71‑1 (MQ=255) ttGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTGAAGATTTCGa > 1:4259491/1‑70 (MQ=255) gCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTGAAGATTTCGaaaa > 1:4234213/1‑71 (MQ=255) gCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTGAAGATTTCGaaaa > 1:1475855/1‑71 (MQ=255) gCTCAAATTACGAGCAAACATACAGGAATAAATCGAt < 1:983957/37‑1 (MQ=255) gCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTGAAGATTTCGaa < 1:1312194/64‑1 (MQ=255) cTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACaa < 1:4422926/47‑1 (MQ=255) tCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACaa < 1:742175/46‑1 (MQ=255) tCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTGaa < 1:1523613/53‑1 (MQ=255) tCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTGaa < 1:1910000/53‑1 (MQ=255) aaaTTACGAGCAAACATACAGGAATAAATCGATGa > 1:2314920/1‑35 (MQ=255) aaaTTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTGAAGAt < 1:429467/54‑1 (MQ=255) aaaTTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTGAAGATTTCGaaaa > 1:2601822/1‑62 (MQ=255) aaaTTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTGAAGATTTCGaaaa > 1:3722646/1‑62 (MQ=255) aaaTTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTGAAGATTTCGAAAACTCTgctgg < 1:3567076/71‑1 (MQ=255) aaTTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTGAAGATTTCGAAAActc < 1:3394589/64‑1 (MQ=255) aTTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTGAAGATTTCGa < 1:4117956/57‑1 (MQ=255) aTTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTGAAGATTTCGAAAACTCTgctggct > 1:722802/1‑71 (MQ=255) aTTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTGAAGATTTCGAAAACTCTgctggct > 1:1087654/1‑71 (MQ=255) tACGAGCAAAAATACAGGAATAAATCGATGAATATGACAAGa > 1:1526700/1‑42 (MQ=37) | TTGACTTCTGTAGAGGGTGAAGTCTCTCCCTATTCAGCAATGCAACCTCGTGTTGCCAGGCTCAAATTACGAGCAAACATACAGGAATAAATCGATGACTATGACAAGACTGAAGATTTCGAAAACTCTGCTGGCT > W3110S.gb/4615984‑4616119 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |