| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,635,047 | G→A | 25.0% | A53V (GCG→GTG) | yjjK ← | fused predicted transporter subunits and ATP‑binding components of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,635,047 | 0 | G | A | 25.0% | 78.4 / 25.2 | 44 | A53V (GCG→GTG) | yjjK | fused predicted transporter subunits and ATP‑binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base G (19/14); new base A (9/2); total (28/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.78e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
CGGCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCA > W3110S.gb/4634980‑4635112 | cGGCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCa > 1:2483553/1‑71 (MQ=255) ggCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCAt < 1:2815663/71‑1 (MQ=255) ggCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCACCAt < 1:2089191/71‑1 (MQ=255) aTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATgc > 1:1645618/1‑71 (MQ=255) ccAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATaa > 1:2857664/1‑64 (MQ=255) ccAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATaa > 1:3625379/1‑64 (MQ=255) cTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAggg > 1:1670870/1‑71 (MQ=255) ttGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAggg > 1:3906783/1‑70 (MQ=255) gATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTg > 1:4219838/1‑70 (MQ=255) tGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGa < 1:790540/70‑1 (MQ=255) tCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACtt < 1:4147070/71‑1 (MQ=255) tGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTAc > 1:156191/1‑71 (MQ=255) cTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGACCGCCATAATGCGCAGCAGGGTGGACTTACCcg > 1:1468932/1‑71 (MQ=255) cGGACGCGCTTCACCTTCGATGTCTTTATCAATGGCCGCCATAATGCGCAGCAggg > 1:3602158/1‑56 (MQ=255) gACGCGCTTCACCTTCGATGTCTTTATCAATGCCCACCATAATGCGCAGCAGGGTGGACTTACCCGCGCCa > 1:4271186/1‑71 (MQ=255) cgcgCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGTACTTACCCGCGCCAtt < 1:1616207/71‑1 (MQ=255) cgcgCTTCACCTTCGATGTCTTTATCAATGCCCACCATAATGCGCAGCAGGGTGGACTTACCCGCGCCAtt > 1:1769978/1‑71 (MQ=255) gcgcTTCACCTTCGATGTCTTTATCAATGCCCGCCa < 1:2142280/36‑1 (MQ=255) gcgcTTCACCTTCGATGTCTTTATCAATGCCCACCATAATGCGCAGCAGGGTGGACTTACCCGCGCCAtt > 1:4539300/1‑70 (MQ=255) gcTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAg < 1:3187847/71‑1 (MQ=255) tCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGcagcag > 1:2323582/1‑44 (MQ=255) tCACCTTCGATGTCTTTATCAATGCCCACCATAATGCGcagcag > 1:3280258/1‑44 (MQ=255) ccTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAg < 1:2745489/71‑1 (MQ=255) ccTTCGATGTCTTTATCAATGCCCACCATAATgcg < 1:3139122/35‑1 (MQ=255) ccTTCGATGTCTTTATCAATGCCCACCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCa > 1:1815502/1‑64 (MQ=255) ttCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGa > 1:17623/1‑71 (MQ=255) ttCGATGTCTTTATCAATGCCCACCATAATGCGcagcag > 1:3805320/1‑39 (MQ=255) ttCGATGTCTTTATCAATGCCCACCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCa > 1:1101857/1‑68 (MQ=255) tCGATGTCTTTATCAATGCCCGCCATAATGCGcagcag < 1:3364763/38‑1 (MQ=255) tCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGa > 1:4469405/1‑70 (MQ=255) cGATGTCTTTATCAATGCCCACCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGaca > 1:403298/1‑71 (MQ=255) cGATGTCTTTATCAATGCCCACCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGaca > 1:3411522/1‑71 (MQ=255) gTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAg > 1:4072836/1‑63 (MQ=255) tCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGAc > 1:4246663/1‑39 (MQ=255) tCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAAt < 1:2739225/71‑1 (MQ=255) tCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAAt < 1:3197969/71‑1 (MQ=255) ttATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAg < 1:162730/59‑1 (MQ=255) tATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCGGCGCCATTCAGACCCAGGACACCAAttttt > 1:4628775/1‑71 (MQ=255) tATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAAttttt > 1:873593/1‑71 (MQ=255) tATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAAtt < 1:4488424/68‑1 (MQ=255) tCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTg < 1:2203455/70‑1 (MQ=255) tGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCa < 1:3155234/71‑1 (MQ=255) cccGCCATAATGCGCAGCAGGGTGGACTTACCCgcgc > 1:3477852/1‑37 (MQ=255) cccGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGAc > 1:2371063/1‑46 (MQ=255) | CGGCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCA > W3110S.gb/4634980‑4635112 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |