| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 301,283 | A→C | 10.9% | V172G (GTG→GGG) | yagT ← | predicted xanthine dehydrogenase, 2Fe‑2S subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 301,283 | 0 | A | C | 10.9% | 117.5 / 11.0 | 55 | V172G (GTG→GGG) | yagT | predicted xanthine dehydrogenase, 2Fe‑2S subunit |
| Reads supporting (aligned to +/- strand): ref base A (22/27); new base C (3/3); total (25/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATA > W3110S.gb/301217‑301348 | gttTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGc > 1:36216/1‑71 (MQ=255)gttTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGc > 1:3368661/1‑71 (MQ=255) tCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCAc > 1:1440820/1‑65 (MQ=255) tCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTAc > 1:3732658/1‑71 (MQ=255) gAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACt < 1:3635589/68‑1 (MQ=255) gAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACt < 1:3009258/68‑1 (MQ=255) cGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGc < 1:2309910/61‑1 (MQ=255) cGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGc < 1:153853/61‑1 (MQ=255) aaaCCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCa < 1:3664576/54‑1 (MQ=255) aaCCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCCCCGCTACTGATGAGCaaa > 1:285191/1‑71 (MQ=255) aaCCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCaaa < 1:1112477/71‑1 (MQ=255) aaCCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCaaa < 1:705944/71‑1 (MQ=255) aCCAAATCGACCGTGACGTGACTGGGAATGCCCTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAAt < 1:2112096/71‑1 (MQ=255) ccAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCaaa < 1:3291747/69‑1 (MQ=255) cAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCa > 1:4045708/1‑50 (MQ=255) aaTCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGc < 1:2475283/71‑1 (MQ=255) aTCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTa > 1:128706/1‑53 (MQ=255) aTCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTa > 1:489764/1‑53 (MQ=255) gACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCgg < 1:2345008/71‑1 (MQ=255) cctgacgtgacTGGGAATGCCGTCCTGAATCTCTTTTAGcccc < 1:527671/41‑1 (MQ=38) tgacgtgacTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTg < 1:820650/71‑1 (MQ=255) gacgtgacTGGGAATGCCGTCCTGAATCTCTTTTAGCCCCGCTACtgatga > 1:2448013/1‑51 (MQ=255) gacgtgacTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTg > 1:4028378/1‑70 (MQ=255) acgtgacTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGc < 1:2032751/41‑1 (MQ=255) acgtgacTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGc < 1:1748404/70‑1 (MQ=255) acgtgacTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCa < 1:865858/71‑1 (MQ=255) gtgacTGGGAATGCCGTCCTGAATCTCTTTTAGCAc > 1:2534917/1‑36 (MQ=255) gtgacTGGGAATGCCGTCCTGAATCTCTTTTAGCAc > 1:1351747/1‑36 (MQ=255) gggAATGCCGTCCTGAATCTCTTTTAGCCCCGCTACTGATGAGCAAATTTGcc < 1:2034872/53‑1 (MQ=255) ggAATGCCGTCCTGAATCTCTTTTAGCCCCGCTACTGATGAGCAAATTTGcc < 1:3375388/52‑1 (MQ=255) ggAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTTCAGTAGCCg < 1:4607378/69‑1 (MQ=255) ggAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCg < 1:3830378/69‑1 (MQ=255) aTGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAg > 1:1873791/1‑60 (MQ=255) aTGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGt > 1:3211641/1‑61 (MQ=255) aTGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGt > 1:73781/1‑61 (MQ=255) tGCCGTCCTGAATCTCTTTTAGCACCGCTACtgatg < 1:2540998/36‑1 (MQ=255) ccGTCCTGAATCTCTTTTAGCCCCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTgg > 1:1149696/1‑69 (MQ=255) ccGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCAc > 1:4542570/1‑66 (MQ=255) ccGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTgg > 1:748954/1‑69 (MQ=255) cGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAg < 1:2994137/71‑1 (MQ=255) tCCTGAATCTCTTTTAGCCCCGCTACTGATGAGCAAATTTGcc < 1:1928571/43‑1 (MQ=255) cTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTa < 1:1389519/54‑1 (MQ=255) cTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCcat > 1:2143418/1‑71 (MQ=255) tGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCcat > 1:439872/1‑70 (MQ=255) tGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCcat > 1:2257226/1‑70 (MQ=255) tctcTTTTAGCACCGCTACTGATGAGCAAATTTGCCCgg < 1:866826/39‑1 (MQ=255) tctcTTTTAGCACCGCTACTGATGAGCAAATTTGCCCgg < 1:2021903/39‑1 (MQ=255) tctcTTTTAGCACCGCTACTGATGAGCAAATTTGCCCg < 1:1073862/38‑1 (MQ=255) tctcTTTTAGCACCGCTACTGATGAGCAAATTTGCCCg < 1:189348/38‑1 (MQ=255) tctcTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGc < 1:4634602/45‑1 (MQ=255) tctcTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCcatca > 1:1641291/1‑68 (MQ=255) ctctTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCt > 1:1770846/1‑71 (MQ=255) tctTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCAc > 1:2332513/1‑54 (MQ=255) ttttAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCcat < 1:3063432/62‑1 (MQ=255) ttAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCcatca > 1:3729322/1‑62 (MQ=255) ttAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCcatca > 1:4468168/1‑62 (MQ=255) tAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATa < 1:411538/70‑1 (MQ=255) tAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATa < 1:2665450/70‑1 (MQ=255) | GTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATA > W3110S.gb/301217‑301348 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |